Experiment: 1-ethyl-3-methylimidazolium chloride 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1207 and Shew_1208 are separated by 3 nucleotides Shew_1208 and Shew_1209 are separated by 9 nucleotides Shew_1209 and Shew_1210 overlap by 8 nucleotides Shew_1210 and Shew_1211 overlap by 19 nucleotides
Shew_1207: Shew_1207 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (RefSeq), at 1,402,081 to 1,402,809
_1207
Shew_1208: Shew_1208 - 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (RefSeq), at 1,402,813 to 1,403,298
_1208
Shew_1209: Shew_1209 - pseudouridylate synthase (RefSeq), at 1,403,308 to 1,404,375
_1209
Shew_1210: Shew_1210 - stationary-phase survival protein SurE (RefSeq), at 1,404,368 to 1,405,117
_1210
Shew_1211: Shew_1211 - protein-L-isoaspartate O-methyltransferase (RefSeq), at 1,405,099 to 1,405,749
_1211
Position (kb) 1403
1404
1405 Strain fitness (log2 ratio) -1
0
1
2 at 1403.277 kb on + strand at 1403.544 kb on + strand, within Shew_1209 at 1403.561 kb on + strand, within Shew_1209 at 1403.659 kb on - strand, within Shew_1209 at 1403.700 kb on - strand, within Shew_1209 at 1403.738 kb on - strand, within Shew_1209 at 1404.427 kb on + strand at 1404.481 kb on + strand, within Shew_1210 at 1404.569 kb on - strand, within Shew_1210 at 1405.267 kb on + strand, within Shew_1211 at 1405.302 kb on + strand, within Shew_1211 at 1405.310 kb on - strand, within Shew_1211
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 50 mM remove 1,403,277 + +0.4 1,403,544 + Shew_1209 0.22 +0.1 1,403,561 + Shew_1209 0.24 -0.1 1,403,659 - Shew_1209 0.33 +1.6 1,403,700 - Shew_1209 0.37 +1.0 1,403,738 - Shew_1209 0.40 +1.3 1,404,427 + +1.1 1,404,481 + Shew_1210 0.15 +1.3 1,404,569 - Shew_1210 0.27 +1.9 1,405,267 + Shew_1211 0.26 -0.3 1,405,302 + Shew_1211 0.31 -0.2 1,405,310 - Shew_1211 0.32 -0.3
Or see this region's nucleotide sequence