Experiment: 1-ethyl-3-methylimidazolium chloride 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1014 and Shew_1015 are separated by 51 nucleotides Shew_1015 and Shew_1016 are separated by 75 nucleotides Shew_1016 and Shew_1017 overlap by 8 nucleotides
Shew_1014: Shew_1014 - XRE family transcriptional regulator (RefSeq), at 1,173,869 to 1,174,375
_1014
Shew_1015: Shew_1015 - Cl- channel, voltage-gated family protein (RefSeq), at 1,174,427 to 1,176,136
_1015
Shew_1016: Shew_1016 - hypothetical protein (RefSeq), at 1,176,212 to 1,177,129
_1016
Shew_1017: Shew_1017 - iron-sulfur cluster insertion protein ErpA (RefSeq), at 1,177,122 to 1,177,472
_1017
Position (kb)
1174
1175
1176
1177 Strain fitness (log2 ratio)
-2
-1
0
1 at 1174.232 kb on + strand, within Shew_1014 at 1174.573 kb on + strand at 1174.598 kb on + strand, within Shew_1015 at 1174.966 kb on - strand, within Shew_1015 at 1175.302 kb on + strand, within Shew_1015 at 1175.416 kb on + strand, within Shew_1015 at 1176.013 kb on + strand at 1176.829 kb on + strand, within Shew_1016 at 1176.854 kb on - strand, within Shew_1016 at 1176.895 kb on - strand, within Shew_1016 at 1176.983 kb on - strand, within Shew_1016
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 50 mM remove 1,174,232 + Shew_1014 0.72 +0.2 1,174,573 + -2.4 1,174,598 + Shew_1015 0.10 +0.6 1,174,966 - Shew_1015 0.32 +0.1 1,175,302 + Shew_1015 0.51 +0.6 1,175,416 + Shew_1015 0.58 +1.1 1,176,013 + +0.3 1,176,829 + Shew_1016 0.67 -0.3 1,176,854 - Shew_1016 0.70 +0.2 1,176,895 - Shew_1016 0.74 +0.5 1,176,983 - Shew_1016 0.84 -0.3
Or see this region's nucleotide sequence