Experiment: 1-ethyl-3-methylimidazolium chloride 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0683 and Shew_0684 are separated by 397 nucleotides Shew_0684 and Shew_0685 are separated by 66 nucleotides Shew_0685 and Shew_0686 are separated by 88 nucleotides
Shew_0683: Shew_0683 - secretion protein HlyD family protein (RefSeq), at 777,423 to 778,487
_0683
Shew_0684: Shew_0684 - hypothetical protein (RefSeq), at 778,885 to 779,292
_0684
Shew_0685: Shew_0685 - hypothetical protein (RefSeq), at 779,359 to 780,066
_0685
Shew_0686: Shew_0686 - DSBA oxidoreductase (RefSeq), at 780,155 to 780,817
_0686
Position (kb) 778
779
780 Strain fitness (log2 ratio) -1
0
1 at 777.954 kb on + strand, within Shew_0683 at 778.061 kb on - strand, within Shew_0683 at 778.313 kb on + strand, within Shew_0683 at 778.359 kb on + strand, within Shew_0683 at 778.359 kb on + strand, within Shew_0683 at 778.388 kb on - strand at 778.664 kb on + strand at 778.982 kb on + strand, within Shew_0684 at 779.027 kb on - strand, within Shew_0684 at 779.238 kb on + strand, within Shew_0684 at 779.286 kb on + strand at 779.286 kb on + strand at 779.286 kb on + strand at 779.294 kb on - strand at 779.404 kb on + strand at 779.414 kb on + strand at 779.507 kb on - strand, within Shew_0685 at 779.521 kb on - strand, within Shew_0685 at 779.647 kb on - strand, within Shew_0685 at 779.885 kb on - strand, within Shew_0685
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 50 mM remove 777,954 + Shew_0683 0.50 +0.3 778,061 - Shew_0683 0.60 +0.5 778,313 + Shew_0683 0.84 -0.1 778,359 + Shew_0683 0.88 +0.4 778,359 + Shew_0683 0.88 +0.5 778,388 - -0.0 778,664 + +0.6 778,982 + Shew_0684 0.24 +0.5 779,027 - Shew_0684 0.35 +0.0 779,238 + Shew_0684 0.87 +1.2 779,286 + +0.0 779,286 + +0.7 779,286 + +0.3 779,294 - +0.2 779,404 + -0.0 779,414 + +0.3 779,507 - Shew_0685 0.21 -0.1 779,521 - Shew_0685 0.23 -0.4 779,647 - Shew_0685 0.41 -0.2 779,885 - Shew_0685 0.74 +0.3
Or see this region's nucleotide sequence