Experiment: 1-ethyl-3-methylimidazolium chloride 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0119 and Shew_0120 are separated by 172 nucleotides Shew_0120 and Shew_0121 are separated by 40 nucleotides Shew_0121 and Shew_0122 are separated by 15 nucleotides Shew_0122 and Shew_0123 overlap by 4 nucleotides
Shew_0119: Shew_0119 - hypothetical protein (RefSeq), at 130,278 to 130,829
_0119
Shew_0120: Shew_0120 - hypothetical protein (RefSeq), at 131,002 to 131,757
_0120
Shew_0121: Shew_0121 - hypothetical protein (RefSeq), at 131,798 to 131,974
_0121
Shew_0122: Shew_0122 - cytochrome c oxidase, subunit III (RefSeq), at 131,990 to 132,865
_0122
Shew_0123: Shew_0123 - cytochrome C oxidase assembly protein (RefSeq), at 132,862 to 133,431
_0123
Position (kb)
131
132 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 131.161 kb on + strand, within Shew_0120 at 131.258 kb on - strand, within Shew_0120 at 131.404 kb on - strand, within Shew_0120 at 131.452 kb on - strand, within Shew_0120 at 131.803 kb on - strand at 131.898 kb on + strand, within Shew_0121 at 131.949 kb on - strand, within Shew_0121 at 132.204 kb on + strand, within Shew_0122 at 132.244 kb on - strand, within Shew_0122 at 132.280 kb on + strand, within Shew_0122 at 132.343 kb on - strand, within Shew_0122 at 132.397 kb on + strand, within Shew_0122 at 132.410 kb on - strand, within Shew_0122 at 132.414 kb on + strand, within Shew_0122 at 132.422 kb on - strand, within Shew_0122 at 132.489 kb on - strand, within Shew_0122 at 132.521 kb on + strand, within Shew_0122 at 132.521 kb on + strand, within Shew_0122 at 132.545 kb on + strand, within Shew_0122 at 132.639 kb on - strand, within Shew_0122 at 132.669 kb on + strand, within Shew_0122 at 132.680 kb on + strand, within Shew_0122 at 132.737 kb on + strand, within Shew_0122 at 132.815 kb on - strand at 132.845 kb on - strand at 132.929 kb on - strand, within Shew_0123 at 132.949 kb on - strand, within Shew_0123
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 50 mM remove 131,161 + Shew_0120 0.21 -1.2 131,258 - Shew_0120 0.34 +0.1 131,404 - Shew_0120 0.53 +0.1 131,452 - Shew_0120 0.60 +0.1 131,803 - +0.4 131,898 + Shew_0121 0.56 -0.6 131,949 - Shew_0121 0.85 -0.0 132,204 + Shew_0122 0.24 -0.2 132,244 - Shew_0122 0.29 +0.1 132,280 + Shew_0122 0.33 -0.4 132,343 - Shew_0122 0.40 -0.5 132,397 + Shew_0122 0.46 -0.3 132,410 - Shew_0122 0.48 -0.1 132,414 + Shew_0122 0.48 -0.4 132,422 - Shew_0122 0.49 -0.0 132,489 - Shew_0122 0.57 +0.3 132,521 + Shew_0122 0.61 -2.9 132,521 + Shew_0122 0.61 +0.0 132,545 + Shew_0122 0.63 +1.0 132,639 - Shew_0122 0.74 -0.1 132,669 + Shew_0122 0.78 -0.5 132,680 + Shew_0122 0.79 +0.1 132,737 + Shew_0122 0.85 +0.1 132,815 - +0.2 132,845 - -0.2 132,929 - Shew_0123 0.12 +0.2 132,949 - Shew_0123 0.15 -0.1
Or see this region's nucleotide sequence