Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS31195 and AZOBR_RS31200 are separated by 6 nucleotides AZOBR_RS31200 and AZOBR_RS31205 are separated by 4 nucleotides AZOBR_RS31205 and AZOBR_RS31210 overlap by 4 nucleotides
AZOBR_RS31195: AZOBR_RS31195 - peptidyl-tRNA hydrolase, at 362,883 to 363,458
_RS31195
AZOBR_RS31200: AZOBR_RS31200 - sugar ABC transporter permease, at 363,465 to 364,442
_RS31200
AZOBR_RS31205: AZOBR_RS31205 - sugar ABC transporter permease, at 364,447 to 365,511
_RS31205
AZOBR_RS31210: AZOBR_RS31210 - sugar ABC transporter ATP-binding protein, at 365,508 to 367,058
_RS31210
Position (kb) 364
365
366 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 363.457 kb on - strand at 363.601 kb on + strand, within AZOBR_RS31200 at 363.601 kb on + strand, within AZOBR_RS31200 at 363.602 kb on - strand, within AZOBR_RS31200 at 363.602 kb on - strand, within AZOBR_RS31200 at 363.653 kb on - strand, within AZOBR_RS31200 at 363.653 kb on - strand, within AZOBR_RS31200 at 363.658 kb on + strand, within AZOBR_RS31200 at 363.751 kb on + strand, within AZOBR_RS31200 at 363.803 kb on - strand, within AZOBR_RS31200 at 363.803 kb on - strand, within AZOBR_RS31200 at 363.803 kb on - strand, within AZOBR_RS31200 at 364.853 kb on + strand, within AZOBR_RS31205 at 364.853 kb on + strand, within AZOBR_RS31205 at 364.853 kb on + strand, within AZOBR_RS31205 at 364.854 kb on - strand, within AZOBR_RS31205 at 365.094 kb on + strand, within AZOBR_RS31205 at 366.424 kb on + strand, within AZOBR_RS31210 at 366.425 kb on - strand, within AZOBR_RS31210 at 366.425 kb on - strand, within AZOBR_RS31210
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 363,457 - -0.1 363,601 + AZOBR_RS31200 0.14 -3.2 363,601 + AZOBR_RS31200 0.14 +0.2 363,602 - AZOBR_RS31200 0.14 -0.4 363,602 - AZOBR_RS31200 0.14 +0.8 363,653 - AZOBR_RS31200 0.19 +0.4 363,653 - AZOBR_RS31200 0.19 +0.6 363,658 + AZOBR_RS31200 0.20 +1.0 363,751 + AZOBR_RS31200 0.29 +0.2 363,803 - AZOBR_RS31200 0.35 -0.4 363,803 - AZOBR_RS31200 0.35 +2.2 363,803 - AZOBR_RS31200 0.35 +0.7 364,853 + AZOBR_RS31205 0.38 -2.6 364,853 + AZOBR_RS31205 0.38 -0.7 364,853 + AZOBR_RS31205 0.38 -0.9 364,854 - AZOBR_RS31205 0.38 +0.1 365,094 + AZOBR_RS31205 0.61 +1.2 366,424 + AZOBR_RS31210 0.59 -0.9 366,425 - AZOBR_RS31210 0.59 -0.3 366,425 - AZOBR_RS31210 0.59 -0.2
Or see this region's nucleotide sequence