Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS29785 and AZOBR_RS29790 are separated by 159 nucleotides AZOBR_RS29790 and AZOBR_RS29795 are separated by 142 nucleotides
AZOBR_RS29785: AZOBR_RS29785 - hypothetical protein, at 31,772 to 32,692
_RS29785
AZOBR_RS29790: AZOBR_RS29790 - 3-ketoacyl-ACP reductase, at 32,852 to 33,604
_RS29790
AZOBR_RS29795: AZOBR_RS29795 - dehydratase, at 33,747 to 34,952
_RS29795
Position (kb) 32
33
34 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 31.916 kb on + strand, within AZOBR_RS29785 at 31.917 kb on - strand, within AZOBR_RS29785 at 31.917 kb on - strand, within AZOBR_RS29785 at 32.120 kb on + strand, within AZOBR_RS29785 at 32.120 kb on + strand, within AZOBR_RS29785 at 32.121 kb on - strand, within AZOBR_RS29785 at 32.121 kb on - strand, within AZOBR_RS29785 at 32.211 kb on - strand, within AZOBR_RS29785 at 32.211 kb on - strand, within AZOBR_RS29785 at 32.595 kb on + strand, within AZOBR_RS29785 at 32.763 kb on + strand at 32.764 kb on - strand at 32.787 kb on - strand at 32.828 kb on + strand at 32.828 kb on + strand at 32.828 kb on + strand at 32.828 kb on + strand at 32.829 kb on - strand at 32.829 kb on - strand at 32.829 kb on - strand at 32.829 kb on - strand at 33.203 kb on + strand, within AZOBR_RS29790 at 33.203 kb on + strand, within AZOBR_RS29790 at 33.204 kb on - strand, within AZOBR_RS29790 at 33.204 kb on - strand, within AZOBR_RS29790 at 33.204 kb on - strand, within AZOBR_RS29790 at 33.204 kb on - strand, within AZOBR_RS29790 at 33.204 kb on - strand, within AZOBR_RS29790 at 33.204 kb on - strand, within AZOBR_RS29790 at 33.209 kb on + strand, within AZOBR_RS29790 at 33.209 kb on + strand, within AZOBR_RS29790 at 33.209 kb on + strand, within AZOBR_RS29790 at 33.210 kb on - strand, within AZOBR_RS29790 at 33.210 kb on - strand, within AZOBR_RS29790 at 33.312 kb on - strand, within AZOBR_RS29790 at 33.312 kb on - strand, within AZOBR_RS29790 at 33.312 kb on - strand, within AZOBR_RS29790 at 33.312 kb on - strand, within AZOBR_RS29790 at 33.312 kb on - strand, within AZOBR_RS29790 at 33.534 kb on - strand at 33.695 kb on + strand at 33.695 kb on + strand at 33.696 kb on - strand at 33.696 kb on - strand at 33.880 kb on - strand, within AZOBR_RS29795 at 33.918 kb on + strand, within AZOBR_RS29795 at 33.919 kb on - strand, within AZOBR_RS29795 at 34.068 kb on + strand, within AZOBR_RS29795 at 34.149 kb on + strand, within AZOBR_RS29795 at 34.150 kb on - strand, within AZOBR_RS29795 at 34.150 kb on - strand, within AZOBR_RS29795 at 34.150 kb on - strand, within AZOBR_RS29795 at 34.419 kb on + strand, within AZOBR_RS29795 at 34.419 kb on + strand, within AZOBR_RS29795 at 34.419 kb on + strand, within AZOBR_RS29795
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 31,916 + AZOBR_RS29785 0.16 -0.4 31,917 - AZOBR_RS29785 0.16 -0.6 31,917 - AZOBR_RS29785 0.16 -1.0 32,120 + AZOBR_RS29785 0.38 -0.4 32,120 + AZOBR_RS29785 0.38 +0.9 32,121 - AZOBR_RS29785 0.38 -1.8 32,121 - AZOBR_RS29785 0.38 -1.8 32,211 - AZOBR_RS29785 0.48 +0.0 32,211 - AZOBR_RS29785 0.48 +0.5 32,595 + AZOBR_RS29785 0.89 +1.0 32,763 + +0.6 32,764 - +0.0 32,787 - +0.7 32,828 + +1.0 32,828 + -2.0 32,828 + +0.3 32,828 + +0.3 32,829 - +0.4 32,829 - +0.5 32,829 - -0.6 32,829 - -0.4 33,203 + AZOBR_RS29790 0.47 -0.1 33,203 + AZOBR_RS29790 0.47 +0.5 33,204 - AZOBR_RS29790 0.47 -0.1 33,204 - AZOBR_RS29790 0.47 +0.4 33,204 - AZOBR_RS29790 0.47 -1.2 33,204 - AZOBR_RS29790 0.47 -0.5 33,204 - AZOBR_RS29790 0.47 -0.4 33,204 - AZOBR_RS29790 0.47 -1.1 33,209 + AZOBR_RS29790 0.47 -0.8 33,209 + AZOBR_RS29790 0.47 -0.4 33,209 + AZOBR_RS29790 0.47 +1.7 33,210 - AZOBR_RS29790 0.48 -0.8 33,210 - AZOBR_RS29790 0.48 +0.3 33,312 - AZOBR_RS29790 0.61 -0.6 33,312 - AZOBR_RS29790 0.61 -1.1 33,312 - AZOBR_RS29790 0.61 -3.1 33,312 - AZOBR_RS29790 0.61 -4.0 33,312 - AZOBR_RS29790 0.61 -0.3 33,534 - +0.2 33,695 + -0.2 33,695 + -1.9 33,696 - +0.1 33,696 - -1.7 33,880 - AZOBR_RS29795 0.11 -0.1 33,918 + AZOBR_RS29795 0.14 +2.0 33,919 - AZOBR_RS29795 0.14 -0.8 34,068 + AZOBR_RS29795 0.27 -1.8 34,149 + AZOBR_RS29795 0.33 +1.4 34,150 - AZOBR_RS29795 0.33 -2.6 34,150 - AZOBR_RS29795 0.33 +0.7 34,150 - AZOBR_RS29795 0.33 -0.1 34,419 + AZOBR_RS29795 0.56 -1.6 34,419 + AZOBR_RS29795 0.56 +0.1 34,419 + AZOBR_RS29795 0.56 -0.2
Or see this region's nucleotide sequence