Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS29180 and AZOBR_RS29185 are separated by 63 nucleotides AZOBR_RS29185 and AZOBR_RS29190 are separated by 86 nucleotides
AZOBR_RS29180: AZOBR_RS29180 - CoA-transferase, at 672,419 to 673,660
_RS29180
AZOBR_RS29185: AZOBR_RS29185 - aldehyde dehydrogenase, at 673,724 to 675,160
_RS29185
AZOBR_RS29190: AZOBR_RS29190 - hydroxyacid dehydrogenase, at 675,247 to 676,149
_RS29190
Position (kb) 673
674
675
676 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 672.800 kb on + strand, within AZOBR_RS29180 at 672.801 kb on - strand, within AZOBR_RS29180 at 672.836 kb on + strand, within AZOBR_RS29180 at 672.836 kb on + strand, within AZOBR_RS29180 at 672.836 kb on + strand, within AZOBR_RS29180 at 672.854 kb on + strand, within AZOBR_RS29180 at 672.974 kb on + strand, within AZOBR_RS29180 at 672.974 kb on + strand, within AZOBR_RS29180 at 672.975 kb on - strand, within AZOBR_RS29180 at 673.532 kb on + strand, within AZOBR_RS29180 at 673.823 kb on + strand at 673.824 kb on - strand at 674.051 kb on + strand, within AZOBR_RS29185 at 674.204 kb on + strand, within AZOBR_RS29185 at 674.205 kb on - strand, within AZOBR_RS29185 at 674.789 kb on + strand, within AZOBR_RS29185 at 674.789 kb on + strand, within AZOBR_RS29185 at 674.790 kb on - strand, within AZOBR_RS29185 at 675.038 kb on + strand at 675.038 kb on + strand at 675.038 kb on + strand at 675.135 kb on - strand at 675.212 kb on + strand at 675.212 kb on + strand at 675.213 kb on - strand at 675.213 kb on - strand at 675.776 kb on - strand, within AZOBR_RS29190 at 675.776 kb on - strand, within AZOBR_RS29190 at 676.069 kb on + strand at 676.070 kb on - strand at 676.103 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 672,800 + AZOBR_RS29180 0.31 +1.3 672,801 - AZOBR_RS29180 0.31 -1.0 672,836 + AZOBR_RS29180 0.34 +0.4 672,836 + AZOBR_RS29180 0.34 -1.3 672,836 + AZOBR_RS29180 0.34 +1.1 672,854 + AZOBR_RS29180 0.35 +0.7 672,974 + AZOBR_RS29180 0.45 -1.5 672,974 + AZOBR_RS29180 0.45 +2.3 672,975 - AZOBR_RS29180 0.45 +0.1 673,532 + AZOBR_RS29180 0.90 +0.1 673,823 + +1.0 673,824 - -2.8 674,051 + AZOBR_RS29185 0.23 +0.2 674,204 + AZOBR_RS29185 0.33 +2.9 674,205 - AZOBR_RS29185 0.33 +0.8 674,789 + AZOBR_RS29185 0.74 -1.6 674,789 + AZOBR_RS29185 0.74 +1.7 674,790 - AZOBR_RS29185 0.74 +0.8 675,038 + -0.8 675,038 + -0.2 675,038 + +1.9 675,135 - -2.5 675,212 + -2.5 675,212 + +0.5 675,213 - -1.9 675,213 - -1.8 675,776 - AZOBR_RS29190 0.59 -0.0 675,776 - AZOBR_RS29190 0.59 -2.9 676,069 + +0.5 676,070 - -0.8 676,103 - +1.6
Or see this region's nucleotide sequence