Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS24310 and AZOBR_RS24315 are separated by 31 nucleotides
AZOBR_RS24310: AZOBR_RS24310 - 3-methylitaconate isomerase, at 502,908 to 504,113
_RS24310
AZOBR_RS24315: AZOBR_RS24315 - carboxyvinyl-carboxyphosphonate phosphorylmutase, at 504,145 to 505,005
_RS24315
Position (kb) 502
503
504
505 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 502.030 kb on + strand at 502.031 kb on - strand at 502.109 kb on - strand at 502.109 kb on - strand at 502.109 kb on - strand at 502.109 kb on - strand at 502.109 kb on - strand at 502.109 kb on - strand at 502.126 kb on + strand at 502.274 kb on - strand at 502.315 kb on + strand at 502.525 kb on + strand at 502.708 kb on + strand at 502.708 kb on + strand at 502.708 kb on + strand at 502.709 kb on - strand at 502.709 kb on - strand at 502.709 kb on - strand at 502.709 kb on - strand at 502.944 kb on + strand at 502.944 kb on + strand at 502.944 kb on + strand at 502.944 kb on + strand at 502.944 kb on + strand at 502.944 kb on + strand at 502.944 kb on + strand at 502.944 kb on + strand at 502.944 kb on + strand at 502.945 kb on - strand at 502.945 kb on - strand at 502.945 kb on - strand at 502.945 kb on - strand at 502.945 kb on - strand at 502.945 kb on - strand at 502.946 kb on + strand at 503.163 kb on + strand, within AZOBR_RS24310 at 503.784 kb on + strand, within AZOBR_RS24310 at 503.784 kb on + strand, within AZOBR_RS24310 at 503.785 kb on - strand, within AZOBR_RS24310 at 503.785 kb on - strand, within AZOBR_RS24310 at 504.262 kb on + strand, within AZOBR_RS24315 at 504.262 kb on + strand, within AZOBR_RS24315 at 504.262 kb on + strand, within AZOBR_RS24315 at 504.262 kb on + strand, within AZOBR_RS24315 at 504.262 kb on + strand, within AZOBR_RS24315 at 504.262 kb on + strand, within AZOBR_RS24315 at 504.263 kb on - strand, within AZOBR_RS24315 at 504.263 kb on - strand, within AZOBR_RS24315 at 504.263 kb on - strand, within AZOBR_RS24315 at 504.263 kb on - strand, within AZOBR_RS24315 at 504.263 kb on - strand, within AZOBR_RS24315 at 504.263 kb on - strand, within AZOBR_RS24315 at 504.263 kb on - strand, within AZOBR_RS24315 at 504.424 kb on + strand, within AZOBR_RS24315 at 504.425 kb on - strand, within AZOBR_RS24315 at 504.836 kb on - strand, within AZOBR_RS24315 at 504.836 kb on - strand, within AZOBR_RS24315 at 504.997 kb on + strand at 504.997 kb on + strand at 504.998 kb on - strand at 504.998 kb on - strand at 504.998 kb on - strand at 505.043 kb on + strand at 505.044 kb on - strand at 505.044 kb on - strand at 505.090 kb on - strand at 505.090 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 502,030 + -0.6 502,031 - -0.0 502,109 - -1.7 502,109 - +1.2 502,109 - -0.8 502,109 - -0.1 502,109 - -1.0 502,109 - -1.6 502,126 + -2.0 502,274 - +0.2 502,315 + +0.6 502,525 + -0.2 502,708 + -0.6 502,708 + +1.2 502,708 + -1.8 502,709 - +0.2 502,709 - -2.0 502,709 - -3.1 502,709 - -1.4 502,944 + -1.9 502,944 + -0.5 502,944 + -1.9 502,944 + +0.7 502,944 + -0.5 502,944 + +0.5 502,944 + -3.0 502,944 + -0.6 502,944 + +0.2 502,945 - +1.7 502,945 - -0.1 502,945 - -0.3 502,945 - +1.2 502,945 - -1.4 502,945 - +1.6 502,946 + +0.6 503,163 + AZOBR_RS24310 0.21 +0.3 503,784 + AZOBR_RS24310 0.73 -1.4 503,784 + AZOBR_RS24310 0.73 +2.3 503,785 - AZOBR_RS24310 0.73 -1.6 503,785 - AZOBR_RS24310 0.73 +2.6 504,262 + AZOBR_RS24315 0.14 +0.4 504,262 + AZOBR_RS24315 0.14 +2.2 504,262 + AZOBR_RS24315 0.14 -3.2 504,262 + AZOBR_RS24315 0.14 -0.2 504,262 + AZOBR_RS24315 0.14 -1.5 504,262 + AZOBR_RS24315 0.14 -1.2 504,263 - AZOBR_RS24315 0.14 -1.1 504,263 - AZOBR_RS24315 0.14 -2.5 504,263 - AZOBR_RS24315 0.14 -2.1 504,263 - AZOBR_RS24315 0.14 +1.1 504,263 - AZOBR_RS24315 0.14 +0.4 504,263 - AZOBR_RS24315 0.14 +0.2 504,263 - AZOBR_RS24315 0.14 +1.3 504,424 + AZOBR_RS24315 0.32 -0.5 504,425 - AZOBR_RS24315 0.33 +0.2 504,836 - AZOBR_RS24315 0.80 -0.1 504,836 - AZOBR_RS24315 0.80 -0.2 504,997 + -1.4 504,997 + -0.1 504,998 - +2.1 504,998 - -2.9 504,998 - -0.0 505,043 + -0.4 505,044 - +0.4 505,044 - -2.7 505,090 - -0.1 505,090 - +1.2
Or see this region's nucleotide sequence