Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS23065 and AZOBR_RS23070 are separated by 134 nucleotides
AZOBR_RS23065: AZOBR_RS23065 - LacI family transcription regulator, at 189,761 to 190,846
_RS23065
AZOBR_RS23070: AZOBR_RS23070 - ABC transporter substrate-binding protein, at 190,981 to 192,036
_RS23070
Position (kb) 190
191
192
193 Strain fitness (log2 ratio) -2
-1
0
1
2 at 190.044 kb on + strand, within AZOBR_RS23065 at 190.044 kb on + strand, within AZOBR_RS23065 at 190.044 kb on + strand, within AZOBR_RS23065 at 190.044 kb on + strand, within AZOBR_RS23065 at 190.044 kb on + strand, within AZOBR_RS23065 at 190.045 kb on - strand, within AZOBR_RS23065 at 190.045 kb on - strand, within AZOBR_RS23065 at 190.045 kb on - strand, within AZOBR_RS23065 at 190.045 kb on - strand, within AZOBR_RS23065 at 190.314 kb on + strand, within AZOBR_RS23065 at 190.315 kb on - strand, within AZOBR_RS23065 at 190.662 kb on + strand, within AZOBR_RS23065 at 190.663 kb on - strand, within AZOBR_RS23065 at 190.663 kb on - strand, within AZOBR_RS23065 at 190.663 kb on - strand, within AZOBR_RS23065 at 190.663 kb on - strand, within AZOBR_RS23065 at 190.663 kb on - strand, within AZOBR_RS23065 at 191.128 kb on + strand, within AZOBR_RS23070 at 191.630 kb on - strand, within AZOBR_RS23070 at 191.630 kb on - strand, within AZOBR_RS23070 at 191.630 kb on - strand, within AZOBR_RS23070 at 191.702 kb on - strand, within AZOBR_RS23070 at 191.702 kb on - strand, within AZOBR_RS23070 at 191.962 kb on + strand at 191.962 kb on + strand at 192.260 kb on - strand at 192.260 kb on - strand at 192.481 kb on + strand at 192.481 kb on + strand at 192.481 kb on + strand at 192.482 kb on - strand at 192.988 kb on + strand at 192.988 kb on + strand at 192.988 kb on + strand at 192.988 kb on + strand at 192.989 kb on - strand at 193.009 kb on + strand at 193.009 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 190,044 + AZOBR_RS23065 0.26 +1.0 190,044 + AZOBR_RS23065 0.26 -1.8 190,044 + AZOBR_RS23065 0.26 -1.9 190,044 + AZOBR_RS23065 0.26 +0.1 190,044 + AZOBR_RS23065 0.26 +1.0 190,045 - AZOBR_RS23065 0.26 -1.1 190,045 - AZOBR_RS23065 0.26 -0.7 190,045 - AZOBR_RS23065 0.26 -1.7 190,045 - AZOBR_RS23065 0.26 +1.1 190,314 + AZOBR_RS23065 0.51 -1.6 190,315 - AZOBR_RS23065 0.51 -0.5 190,662 + AZOBR_RS23065 0.83 +0.8 190,663 - AZOBR_RS23065 0.83 +0.2 190,663 - AZOBR_RS23065 0.83 -0.3 190,663 - AZOBR_RS23065 0.83 -1.7 190,663 - AZOBR_RS23065 0.83 +0.0 190,663 - AZOBR_RS23065 0.83 -1.5 191,128 + AZOBR_RS23070 0.14 -1.8 191,630 - AZOBR_RS23070 0.61 +0.4 191,630 - AZOBR_RS23070 0.61 -0.4 191,630 - AZOBR_RS23070 0.61 -1.7 191,702 - AZOBR_RS23070 0.68 +0.6 191,702 - AZOBR_RS23070 0.68 +0.6 191,962 + +0.2 191,962 + -1.5 192,260 - -0.3 192,260 - +1.4 192,481 + -1.1 192,481 + -0.5 192,481 + -1.0 192,482 - +2.6 192,988 + -2.2 192,988 + +1.6 192,988 + +0.6 192,988 + -0.8 192,989 - +0.5 193,009 + +1.4 193,009 + +0.9
Or see this region's nucleotide sequence