Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS16250 and AZOBR_RS16255 are separated by 72 nucleotides AZOBR_RS16255 and AZOBR_RS16260 are separated by 40 nucleotides AZOBR_RS16260 and AZOBR_RS16265 are separated by 77 nucleotides AZOBR_RS16265 and AZOBR_RS16270 are separated by 22 nucleotides
AZOBR_RS16250: AZOBR_RS16250 - LysR family transcriptional regulator, at 397,495 to 398,409
_RS16250
AZOBR_RS16255: AZOBR_RS16255 - membrane protein, at 398,482 to 398,964
_RS16255
AZOBR_RS16260: AZOBR_RS16260 - azoreductase, at 399,005 to 399,604
_RS16260
AZOBR_RS16265: AZOBR_RS16265 - 3-oxoacyl-ACP reductase, at 399,682 to 400,461
_RS16265
AZOBR_RS16270: AZOBR_RS16270 - DNA replication protein, at 400,484 to 401,164
_RS16270
Position (kb)
399
400 Strain fitness (log2 ratio)
-1
0
1 at 398.236 kb on + strand, within AZOBR_RS16250 at 398.734 kb on + strand, within AZOBR_RS16255 at 398.735 kb on - strand, within AZOBR_RS16255 at 398.735 kb on - strand, within AZOBR_RS16255 at 398.735 kb on - strand, within AZOBR_RS16255 at 399.266 kb on + strand, within AZOBR_RS16260 at 399.267 kb on - strand, within AZOBR_RS16260 at 399.408 kb on + strand, within AZOBR_RS16260 at 399.409 kb on - strand, within AZOBR_RS16260
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 398,236 + AZOBR_RS16250 0.81 +0.1 398,734 + AZOBR_RS16255 0.52 +0.3 398,735 - AZOBR_RS16255 0.52 +0.8 398,735 - AZOBR_RS16255 0.52 +1.2 398,735 - AZOBR_RS16255 0.52 +1.1 399,266 + AZOBR_RS16260 0.43 +0.3 399,267 - AZOBR_RS16260 0.44 +1.2 399,408 + AZOBR_RS16260 0.67 +1.6 399,409 - AZOBR_RS16260 0.67 +0.2
Or see this region's nucleotide sequence