Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS14865 and AZOBR_RS14870 are separated by 85 nucleotides AZOBR_RS14870 and AZOBR_RS14875 are separated by 129 nucleotides AZOBR_RS14875 and AZOBR_RS14880 are separated by 100 nucleotides
AZOBR_RS14865: AZOBR_RS14865 - aminopeptidase N, at 107,728 to 110,385
_RS14865
AZOBR_RS14870: AZOBR_RS14870 - cytochrome C transmembrane protein, at 110,471 to 110,851
_RS14870
AZOBR_RS14875: AZOBR_RS14875 - carbon monoxide dehydrogenase subunit G, at 110,981 to 111,643
_RS14875
AZOBR_RS14880: AZOBR_RS14880 - hypothetical protein, at 111,744 to 112,205
_RS14880
Position (kb)
110
111 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 109.631 kb on - strand, within AZOBR_RS14865 at 109.874 kb on - strand, within AZOBR_RS14865 at 109.874 kb on - strand, within AZOBR_RS14865 at 109.969 kb on + strand, within AZOBR_RS14865 at 109.969 kb on + strand, within AZOBR_RS14865 at 110.111 kb on - strand, within AZOBR_RS14865 at 110.111 kb on - strand, within AZOBR_RS14865 at 110.580 kb on + strand, within AZOBR_RS14870 at 110.580 kb on + strand, within AZOBR_RS14870 at 110.581 kb on - strand, within AZOBR_RS14870 at 110.840 kb on - strand at 110.840 kb on - strand at 110.840 kb on - strand at 110.914 kb on + strand at 110.914 kb on + strand at 110.914 kb on + strand at 110.914 kb on + strand at 110.915 kb on - strand at 110.915 kb on - strand at 110.915 kb on - strand at 110.929 kb on + strand at 110.930 kb on - strand at 111.045 kb on + strand at 111.045 kb on + strand at 111.046 kb on - strand at 111.046 kb on - strand at 111.423 kb on + strand, within AZOBR_RS14875 at 111.423 kb on + strand, within AZOBR_RS14875 at 111.424 kb on - strand, within AZOBR_RS14875 at 111.424 kb on - strand, within AZOBR_RS14875 at 111.680 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 109,631 - AZOBR_RS14865 0.72 +1.2 109,874 - AZOBR_RS14865 0.81 -0.8 109,874 - AZOBR_RS14865 0.81 -1.8 109,969 + AZOBR_RS14865 0.84 -0.8 109,969 + AZOBR_RS14865 0.84 +3.2 110,111 - AZOBR_RS14865 0.90 -0.5 110,111 - AZOBR_RS14865 0.90 +0.1 110,580 + AZOBR_RS14870 0.29 -0.6 110,580 + AZOBR_RS14870 0.29 +0.7 110,581 - AZOBR_RS14870 0.29 -0.6 110,840 - -0.2 110,840 - +0.8 110,840 - -0.3 110,914 + +0.4 110,914 + -1.3 110,914 + -0.8 110,914 + -0.3 110,915 - +0.2 110,915 - +0.5 110,915 - +0.5 110,929 + +0.4 110,930 - +0.6 111,045 + -1.5 111,045 + +0.6 111,046 - +0.4 111,046 - -0.3 111,423 + AZOBR_RS14875 0.67 -3.2 111,423 + AZOBR_RS14875 0.67 +0.2 111,424 - AZOBR_RS14875 0.67 +0.3 111,424 - AZOBR_RS14875 0.67 +0.4 111,680 + -1.6
Or see this region's nucleotide sequence