Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS10545 and AZOBR_RS10550 are separated by 399 nucleotides
AZOBR_RS10545: AZOBR_RS10545 - threonine synthase, at 2,212,234 to 2,213,646
_RS10545
AZOBR_RS10550: AZOBR_RS10550 - 1 4-beta-glucanase, at 2,214,046 to 2,215,137
_RS10550
Position (kb)
2214
2215
2216 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2213.715 kb on - strand at 2213.715 kb on - strand at 2213.719 kb on - strand at 2214.077 kb on + strand at 2214.077 kb on + strand at 2214.155 kb on + strand at 2214.155 kb on + strand at 2214.227 kb on + strand, within AZOBR_RS10550 at 2214.227 kb on + strand, within AZOBR_RS10550 at 2214.248 kb on + strand, within AZOBR_RS10550 at 2214.248 kb on + strand, within AZOBR_RS10550 at 2214.248 kb on + strand, within AZOBR_RS10550 at 2214.248 kb on + strand, within AZOBR_RS10550 at 2214.248 kb on + strand, within AZOBR_RS10550 at 2214.248 kb on + strand, within AZOBR_RS10550 at 2214.249 kb on - strand, within AZOBR_RS10550 at 2214.249 kb on - strand, within AZOBR_RS10550 at 2214.249 kb on - strand, within AZOBR_RS10550 at 2214.249 kb on - strand, within AZOBR_RS10550 at 2214.249 kb on - strand, within AZOBR_RS10550 at 2214.428 kb on + strand, within AZOBR_RS10550 at 2214.689 kb on + strand, within AZOBR_RS10550 at 2214.690 kb on - strand, within AZOBR_RS10550 at 2214.750 kb on - strand, within AZOBR_RS10550 at 2214.833 kb on + strand, within AZOBR_RS10550 at 2214.870 kb on + strand, within AZOBR_RS10550 at 2214.871 kb on - strand, within AZOBR_RS10550 at 2214.871 kb on - strand, within AZOBR_RS10550 at 2215.222 kb on + strand at 2215.222 kb on + strand at 2215.809 kb on + strand at 2215.809 kb on + strand at 2215.809 kb on + strand at 2215.809 kb on + strand at 2215.810 kb on - strand at 2215.883 kb on + strand at 2215.884 kb on - strand at 2215.884 kb on - strand at 2216.001 kb on + strand at 2216.052 kb on + strand at 2216.052 kb on + strand at 2216.053 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,213,715 - -0.0 2,213,715 - +1.3 2,213,719 - -1.0 2,214,077 + -0.1 2,214,077 + -2.4 2,214,155 + -0.3 2,214,155 + -0.1 2,214,227 + AZOBR_RS10550 0.17 -0.5 2,214,227 + AZOBR_RS10550 0.17 +0.1 2,214,248 + AZOBR_RS10550 0.18 +0.7 2,214,248 + AZOBR_RS10550 0.18 -0.9 2,214,248 + AZOBR_RS10550 0.18 +2.1 2,214,248 + AZOBR_RS10550 0.18 +0.6 2,214,248 + AZOBR_RS10550 0.18 -0.2 2,214,248 + AZOBR_RS10550 0.18 +3.0 2,214,249 - AZOBR_RS10550 0.19 +0.3 2,214,249 - AZOBR_RS10550 0.19 -1.2 2,214,249 - AZOBR_RS10550 0.19 +0.3 2,214,249 - AZOBR_RS10550 0.19 +0.1 2,214,249 - AZOBR_RS10550 0.19 -1.2 2,214,428 + AZOBR_RS10550 0.35 +0.0 2,214,689 + AZOBR_RS10550 0.59 -0.2 2,214,690 - AZOBR_RS10550 0.59 -0.9 2,214,750 - AZOBR_RS10550 0.64 -0.1 2,214,833 + AZOBR_RS10550 0.72 +0.7 2,214,870 + AZOBR_RS10550 0.75 -1.2 2,214,871 - AZOBR_RS10550 0.76 -0.2 2,214,871 - AZOBR_RS10550 0.76 +0.1 2,215,222 + +0.2 2,215,222 + +1.3 2,215,809 + -0.6 2,215,809 + -0.7 2,215,809 + +0.9 2,215,809 + -0.3 2,215,810 - -2.8 2,215,883 + -0.6 2,215,884 - -2.0 2,215,884 - +0.4 2,216,001 + -1.0 2,216,052 + -1.4 2,216,052 + +1.8 2,216,053 - +0.1
Or see this region's nucleotide sequence