Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS02325 and AZOBR_RS02335 are separated by 985 nucleotides AZOBR_RS02335 and AZOBR_RS02340 are separated by 174 nucleotides AZOBR_RS02340 and AZOBR_RS02345 are separated by 170 nucleotides AZOBR_RS02345 and AZOBR_RS02350 are separated by 77 nucleotides
AZOBR_RS02325: AZOBR_RS02325 - ABC transporter substrate-binding protein, at 480,050 to 481,561
_RS02325
AZOBR_RS02335: AZOBR_RS02335 - peptide ABC transporter permease, at 482,547 to 483,407
_RS02335
AZOBR_RS02340: AZOBR_RS02340 - xanthine phosphoribosyltransferase, at 483,582 to 484,082
_RS02340
AZOBR_RS02345: AZOBR_RS02345 - tRNA-Arg, at 484,253 to 484,329
_RS02345
AZOBR_RS02350: AZOBR_RS02350 - hypothetical protein, at 484,407 to 484,586
_RS02350
Position (kb) 482
483
484 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 482.236 kb on + strand at 482.237 kb on - strand at 482.237 kb on - strand at 482.237 kb on - strand at 482.237 kb on - strand at 482.237 kb on - strand at 482.413 kb on + strand at 482.413 kb on + strand at 483.207 kb on + strand, within AZOBR_RS02335 at 483.207 kb on + strand, within AZOBR_RS02335 at 483.208 kb on - strand, within AZOBR_RS02335 at 483.208 kb on - strand, within AZOBR_RS02335 at 483.715 kb on + strand, within AZOBR_RS02340 at 483.877 kb on + strand, within AZOBR_RS02340 at 483.877 kb on + strand, within AZOBR_RS02340 at 484.116 kb on - strand at 484.117 kb on + strand at 484.118 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 482,236 + +0.3 482,237 - -0.3 482,237 - -0.3 482,237 - -0.1 482,237 - -0.1 482,237 - -0.2 482,413 + +0.5 482,413 + -1.6 483,207 + AZOBR_RS02335 0.77 +1.8 483,207 + AZOBR_RS02335 0.77 -3.3 483,208 - AZOBR_RS02335 0.77 +2.5 483,208 - AZOBR_RS02335 0.77 -0.4 483,715 + AZOBR_RS02340 0.27 -2.6 483,877 + AZOBR_RS02340 0.59 +0.9 483,877 + AZOBR_RS02340 0.59 -1.9 484,116 - -0.9 484,117 + +0.9 484,118 - -0.3
Or see this region's nucleotide sequence