Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS01575 and AZOBR_RS01580 are separated by 19 nucleotides AZOBR_RS01580 and AZOBR_RS01585 are separated by 71 nucleotides
AZOBR_RS01575: AZOBR_RS01575 - 6-O-methylguanine DNA methyltransferase, at 323,345 to 324,169
_RS01575
AZOBR_RS01580: AZOBR_RS01580 - dihydrodipicolinate reductase, at 324,189 to 324,986
_RS01580
AZOBR_RS01585: AZOBR_RS01585 - zinc transporter, at 325,058 to 326,044
_RS01585
Position (kb) 324
325 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 323.609 kb on + strand, within AZOBR_RS01575 at 323.610 kb on - strand, within AZOBR_RS01575 at 323.610 kb on - strand, within AZOBR_RS01575 at 323.610 kb on - strand, within AZOBR_RS01575 at 324.086 kb on + strand, within AZOBR_RS01575 at 324.086 kb on + strand, within AZOBR_RS01575 at 324.167 kb on + strand at 325.339 kb on - strand, within AZOBR_RS01585 at 325.353 kb on + strand, within AZOBR_RS01585 at 325.359 kb on + strand, within AZOBR_RS01585 at 325.360 kb on - strand, within AZOBR_RS01585 at 325.470 kb on + strand, within AZOBR_RS01585 at 325.618 kb on + strand, within AZOBR_RS01585 at 325.618 kb on + strand, within AZOBR_RS01585 at 325.619 kb on - strand, within AZOBR_RS01585 at 325.619 kb on - strand, within AZOBR_RS01585 at 325.619 kb on - strand, within AZOBR_RS01585 at 325.845 kb on + strand, within AZOBR_RS01585 at 325.845 kb on + strand, within AZOBR_RS01585 at 325.845 kb on + strand, within AZOBR_RS01585 at 325.845 kb on + strand, within AZOBR_RS01585 at 325.845 kb on + strand, within AZOBR_RS01585 at 325.846 kb on - strand, within AZOBR_RS01585 at 325.846 kb on - strand, within AZOBR_RS01585
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 323,609 + AZOBR_RS01575 0.32 -1.2 323,610 - AZOBR_RS01575 0.32 +0.0 323,610 - AZOBR_RS01575 0.32 -2.2 323,610 - AZOBR_RS01575 0.32 -1.0 324,086 + AZOBR_RS01575 0.90 +0.6 324,086 + AZOBR_RS01575 0.90 -2.2 324,167 + -0.3 325,339 - AZOBR_RS01585 0.28 -3.4 325,353 + AZOBR_RS01585 0.30 -1.7 325,359 + AZOBR_RS01585 0.30 -1.2 325,360 - AZOBR_RS01585 0.31 -1.5 325,470 + AZOBR_RS01585 0.42 -1.2 325,618 + AZOBR_RS01585 0.57 -1.3 325,618 + AZOBR_RS01585 0.57 -2.1 325,619 - AZOBR_RS01585 0.57 -1.5 325,619 - AZOBR_RS01585 0.57 -0.5 325,619 - AZOBR_RS01585 0.57 -1.5 325,845 + AZOBR_RS01585 0.80 +0.2 325,845 + AZOBR_RS01585 0.80 +0.5 325,845 + AZOBR_RS01585 0.80 -1.2 325,845 + AZOBR_RS01585 0.80 -0.7 325,845 + AZOBR_RS01585 0.80 -0.5 325,846 - AZOBR_RS01585 0.80 -0.7 325,846 - AZOBR_RS01585 0.80 -1.0
Or see this region's nucleotide sequence