Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS01370 and AZOBR_RS01375 are separated by 11 nucleotides AZOBR_RS01375 and AZOBR_RS01380 are separated by 461 nucleotides AZOBR_RS01380 and AZOBR_RS01385 are separated by 227 nucleotides
AZOBR_RS01370: AZOBR_RS01370 - metallopeptidase, at 284,268 to 285,161
_RS01370
AZOBR_RS01375: AZOBR_RS01375 - hypothetical protein, at 285,173 to 285,685
_RS01375
AZOBR_RS01380: AZOBR_RS01380 - hypothetical protein, at 286,147 to 286,452
_RS01380
AZOBR_RS01385: AZOBR_RS01385 - polynucleotide phosphorylase, at 286,680 to 288,800
_RS01385
Position (kb) 286
287 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 285.160 kb on - strand at 285.160 kb on - strand at 285.394 kb on - strand, within AZOBR_RS01375 at 285.394 kb on - strand, within AZOBR_RS01375 at 285.550 kb on + strand, within AZOBR_RS01375 at 285.550 kb on + strand, within AZOBR_RS01375 at 285.642 kb on + strand at 285.643 kb on - strand at 285.717 kb on - strand at 285.754 kb on + strand at 285.755 kb on - strand at 285.755 kb on - strand at 285.836 kb on + strand at 286.083 kb on - strand at 286.083 kb on - strand at 286.550 kb on + strand at 286.550 kb on + strand at 286.551 kb on - strand at 286.648 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 285,160 - -0.3 285,160 - -4.0 285,394 - AZOBR_RS01375 0.43 -2.6 285,394 - AZOBR_RS01375 0.43 -0.6 285,550 + AZOBR_RS01375 0.73 +0.4 285,550 + AZOBR_RS01375 0.73 -2.5 285,642 + -0.7 285,643 - -2.3 285,717 - -1.4 285,754 + -1.1 285,755 - +1.0 285,755 - -1.6 285,836 + -0.1 286,083 - +0.1 286,083 - -1.1 286,550 + +1.4 286,550 + -0.2 286,551 - -2.5 286,648 + -0.3
Or see this region's nucleotide sequence