Experiment: 1-ethyl-3-methylimidazolium acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3523 and Shew_3524 overlap by 1 nucleotides Shew_3524 and fabG overlap by 4 nucleotides fabG and Shew_3526 overlap by 1 nucleotides
Shew_3523: Shew_3523 - 3-oxoacyl-(acyl carrier protein) synthase I (RefSeq), at 4,210,315 to 4,211,520
_3523
Shew_3524: Shew_3524 - Beta-hydroxyacyl-(acyl-carrier-protein) dehydratase, FabA/FabZ (RefSeq), at 4,211,520 to 4,212,113
_3524
Shew_3525: fabG - 3-ketoacyl-(acyl-carrier-protein) reductase (RefSeq), at 4,212,110 to 4,212,835
fabG
Shew_3526: Shew_3526 - 3-oxoacyl-(acyl carrier protein) synthase II (RefSeq), at 4,212,835 to 4,214,076
_3526
Position (kb)
4211
4212
4213 Strain fitness (log2 ratio)
-1
0
1 at 4210.604 kb on + strand, within Shew_3523 at 4210.716 kb on + strand, within Shew_3523 at 4210.984 kb on + strand, within Shew_3523 at 4211.150 kb on + strand, within Shew_3523 at 4211.263 kb on + strand, within Shew_3523 at 4211.578 kb on - strand at 4211.681 kb on - strand, within Shew_3524 at 4211.778 kb on + strand, within Shew_3524 at 4211.786 kb on - strand, within Shew_3524 at 4212.026 kb on + strand, within Shew_3524 at 4212.173 kb on - strand at 4212.173 kb on - strand at 4212.173 kb on - strand at 4212.530 kb on + strand, within fabG at 4212.530 kb on + strand, within fabG at 4212.530 kb on + strand, within fabG at 4212.620 kb on - strand, within fabG at 4212.712 kb on + strand, within fabG at 4212.983 kb on + strand, within Shew_3526 at 4213.078 kb on + strand, within Shew_3526 at 4213.109 kb on - strand, within Shew_3526
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 10 mM remove 4,210,604 + Shew_3523 0.24 -0.5 4,210,716 + Shew_3523 0.33 -0.3 4,210,984 + Shew_3523 0.55 -0.3 4,211,150 + Shew_3523 0.69 -0.2 4,211,263 + Shew_3523 0.79 -0.2 4,211,578 - +0.7 4,211,681 - Shew_3524 0.27 +0.4 4,211,778 + Shew_3524 0.43 +0.5 4,211,786 - Shew_3524 0.45 +0.4 4,212,026 + Shew_3524 0.85 -0.2 4,212,173 - -1.0 4,212,173 - +1.2 4,212,173 - -0.0 4,212,530 + fabG Shew_3525 0.58 -0.2 4,212,530 + fabG Shew_3525 0.58 -0.3 4,212,530 + fabG Shew_3525 0.58 -0.4 4,212,620 - fabG Shew_3525 0.70 +0.2 4,212,712 + fabG Shew_3525 0.83 -0.3 4,212,983 + Shew_3526 0.12 -0.3 4,213,078 + Shew_3526 0.20 -0.6 4,213,109 - Shew_3526 0.22 -0.3
Or see this region's nucleotide sequence