Experiment: 1-ethyl-3-methylimidazolium acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0375 and Shew_0376 are separated by 594 nucleotides Shew_0376 and Shew_0377 are separated by 99 nucleotides Shew_0377 and argS are separated by 14 nucleotides
Shew_0375: Shew_0375 - ATP-dependent protease peptidase subunit (RefSeq), at 422,504 to 423,028
_0375
Shew_0376: Shew_0376 - curlin-associated protein (RefSeq), at 423,623 to 425,317
_0376
Shew_0377: Shew_0377 - sporulation domain-containing protein (RefSeq), at 425,417 to 425,995
_0377
Shew_0378: argS - arginyl-tRNA synthetase (RefSeq), at 426,010 to 427,755
argS
Position (kb) 423
424
425
426 Strain fitness (log2 ratio) -1
0
1 at 422.748 kb on + strand, within Shew_0375 at 423.158 kb on + strand at 423.412 kb on - strand at 423.541 kb on + strand at 423.572 kb on - strand at 423.742 kb on + strand at 423.862 kb on + strand, within Shew_0376 at 424.506 kb on - strand, within Shew_0376 at 424.542 kb on - strand, within Shew_0376 at 424.575 kb on + strand, within Shew_0376 at 424.583 kb on - strand, within Shew_0376 at 424.685 kb on + strand, within Shew_0376 at 424.965 kb on + strand, within Shew_0376 at 425.140 kb on + strand, within Shew_0376 at 425.311 kb on + strand at 425.319 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 10 mM remove 422,748 + Shew_0375 0.46 +0.8 423,158 + +0.2 423,412 - +0.1 423,541 + +0.2 423,572 - -0.2 423,742 + +0.0 423,862 + Shew_0376 0.14 -1.1 424,506 - Shew_0376 0.52 -0.2 424,542 - Shew_0376 0.54 -0.2 424,575 + Shew_0376 0.56 -0.4 424,583 - Shew_0376 0.57 +0.0 424,685 + Shew_0376 0.63 -0.0 424,965 + Shew_0376 0.79 -0.1 425,140 + Shew_0376 0.89 +0.1 425,311 + +0.0 425,319 - +0.4
Or see this region's nucleotide sequence