Experiment: BHIS with Lomefloxacin 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0087 and BT0088 overlap by 26 nucleotides BT0088 and BT0089 are separated by 3 nucleotides BT0089 and BT0090 are separated by 12 nucleotides
BT0087: BT0087 - conserved protein found in conjugate transposon (NCBI ptt file), at 81,067 to 82,452
BT0087
BT0088: BT0088 - conserved protein found in conjugate transposon (NCBI ptt file), at 82,427 to 82,732
BT0088
BT0089: BT0089 - conserved protein found in conjugate transposon (NCBI ptt file), at 82,736 to 83,359
BT0089
BT0090: BT0090 - conserved protein found in conjugate transposon (NCBI ptt file), at 83,372 to 84,397
BT0090
Position (kb) 82
83 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 82.021 kb on + strand, within BT0087 at 82.287 kb on + strand, within BT0087 at 82.287 kb on + strand, within BT0087 at 82.287 kb on + strand, within BT0087 at 82.477 kb on + strand, within BT0088 at 82.523 kb on + strand, within BT0088 at 82.628 kb on + strand, within BT0088 at 82.807 kb on + strand, within BT0089 at 82.810 kb on + strand, within BT0089 at 82.949 kb on + strand, within BT0089 at 82.949 kb on + strand, within BT0089 at 82.992 kb on + strand, within BT0089 at 83.373 kb on + strand at 83.390 kb on + strand at 83.465 kb on - strand at 83.567 kb on + strand, within BT0090 at 83.572 kb on + strand, within BT0090 at 83.650 kb on + strand, within BT0090 at 83.650 kb on + strand, within BT0090 at 83.721 kb on + strand, within BT0090
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Lomefloxacin 0.025 mM remove 82,021 + BT0087 0.69 -1.1 82,287 + BT0087 0.88 +0.4 82,287 + BT0087 0.88 -0.6 82,287 + BT0087 0.88 +0.2 82,477 + BT0088 0.16 -1.3 82,523 + BT0088 0.31 -2.2 82,628 + BT0088 0.66 -2.3 82,807 + BT0089 0.11 -1.3 82,810 + BT0089 0.12 +0.1 82,949 + BT0089 0.34 -3.4 82,949 + BT0089 0.34 +1.8 82,992 + BT0089 0.41 -2.8 83,373 + -0.8 83,390 + -0.4 83,465 - +0.0 83,567 + BT0090 0.19 +1.0 83,572 + BT0090 0.19 +1.6 83,650 + BT0090 0.27 -1.4 83,650 + BT0090 0.27 +0.2 83,721 + BT0090 0.34 -0.1
Or see this region's nucleotide sequence