Strain Fitness in Azospirillum brasilense Sp245 around AZOBR_RS32215

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAZOBR_RS32200 and AZOBR_RS32205 are separated by 125 nucleotidesAZOBR_RS32205 and AZOBR_RS32210 overlap by 4 nucleotidesAZOBR_RS32210 and AZOBR_RS32215 are separated by 304 nucleotidesAZOBR_RS32215 and AZOBR_RS32220 are separated by 22 nucleotides AZOBR_RS32200: AZOBR_RS32200 - glycerol-3-phosphate dehydrogenase subunit C, at 590,221 to 590,493 _RS32200 AZOBR_RS32205: AZOBR_RS32205 - hypothetical protein, at 590,619 to 590,852 _RS32205 AZOBR_RS32210: AZOBR_RS32210 - hypothetical protein, at 590,849 to 591,100 _RS32210 AZOBR_RS32215: AZOBR_RS32215 - hypothetical protein, at 591,405 to 591,755 _RS32215 AZOBR_RS32220: AZOBR_RS32220 - hypothetical protein, at 591,778 to 592,857 _RS32220 Position (kb) 591 592Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 590.491 kb on + strandat 590.491 kb on + strandat 590.492 kb on - strandat 590.566 kb on - strandat 590.566 kb on - strandat 590.715 kb on + strand, within AZOBR_RS32205at 590.715 kb on + strand, within AZOBR_RS32205at 590.715 kb on + strand, within AZOBR_RS32205at 590.715 kb on + strand, within AZOBR_RS32205at 590.715 kb on + strand, within AZOBR_RS32205at 590.715 kb on + strand, within AZOBR_RS32205at 590.716 kb on - strand, within AZOBR_RS32205at 590.716 kb on - strand, within AZOBR_RS32205at 590.716 kb on - strand, within AZOBR_RS32205at 591.449 kb on - strand, within AZOBR_RS32215at 591.449 kb on - strand, within AZOBR_RS32215at 591.489 kb on - strand, within AZOBR_RS32215at 591.513 kb on + strand, within AZOBR_RS32215at 591.513 kb on + strand, within AZOBR_RS32215at 591.513 kb on + strand, within AZOBR_RS32215at 591.514 kb on - strand, within AZOBR_RS32215at 591.514 kb on - strand, within AZOBR_RS32215at 591.645 kb on + strand, within AZOBR_RS32215at 591.645 kb on + strand, within AZOBR_RS32215at 591.645 kb on + strand, within AZOBR_RS32215at 591.646 kb on - strand, within AZOBR_RS32215at 591.646 kb on - strand, within AZOBR_RS32215at 591.646 kb on - strand, within AZOBR_RS32215at 591.646 kb on - strand, within AZOBR_RS32215at 591.646 kb on - strand, within AZOBR_RS32215at 591.737 kb on + strandat 591.737 kb on + strandat 591.737 kb on + strandat 591.910 kb on + strand, within AZOBR_RS32220at 591.911 kb on - strand, within AZOBR_RS32220at 591.911 kb on - strand, within AZOBR_RS32220at 592.247 kb on - strand, within AZOBR_RS32220at 592.247 kb on - strand, within AZOBR_RS32220at 592.345 kb on + strand, within AZOBR_RS32220at 592.345 kb on + strand, within AZOBR_RS32220at 592.346 kb on - strand, within AZOBR_RS32220at 592.346 kb on - strand, within AZOBR_RS32220at 592.348 kb on + strand, within AZOBR_RS32220at 592.358 kb on - strand, within AZOBR_RS32220at 592.366 kb on + strand, within AZOBR_RS32220at 592.367 kb on - strand, within AZOBR_RS32220

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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590,491 + -0.6
590,491 + +0.5
590,492 - -0.1
590,566 - +0.7
590,566 - -0.5
590,715 + AZOBR_RS32205 0.41 -2.6
590,715 + AZOBR_RS32205 0.41 -0.5
590,715 + AZOBR_RS32205 0.41 -0.8
590,715 + AZOBR_RS32205 0.41 +0.2
590,715 + AZOBR_RS32205 0.41 -3.0
590,715 + AZOBR_RS32205 0.41 +0.2
590,716 - AZOBR_RS32205 0.41 +0.2
590,716 - AZOBR_RS32205 0.41 +1.1
590,716 - AZOBR_RS32205 0.41 +0.5
591,449 - AZOBR_RS32215 0.13 -1.5
591,449 - AZOBR_RS32215 0.13 +1.7
591,489 - AZOBR_RS32215 0.24 +0.5
591,513 + AZOBR_RS32215 0.31 +0.1
591,513 + AZOBR_RS32215 0.31 -1.4
591,513 + AZOBR_RS32215 0.31 +0.2
591,514 - AZOBR_RS32215 0.31 -0.3
591,514 - AZOBR_RS32215 0.31 -0.4
591,645 + AZOBR_RS32215 0.68 -0.8
591,645 + AZOBR_RS32215 0.68 +0.4
591,645 + AZOBR_RS32215 0.68 -1.6
591,646 - AZOBR_RS32215 0.69 -0.2
591,646 - AZOBR_RS32215 0.69 +0.0
591,646 - AZOBR_RS32215 0.69 -0.3
591,646 - AZOBR_RS32215 0.69 -1.6
591,646 - AZOBR_RS32215 0.69 -0.1
591,737 + -0.7
591,737 + -0.8
591,737 + -0.2
591,910 + AZOBR_RS32220 0.12 +0.5
591,911 - AZOBR_RS32220 0.12 -0.9
591,911 - AZOBR_RS32220 0.12 -0.2
592,247 - AZOBR_RS32220 0.43 -2.7
592,247 - AZOBR_RS32220 0.43 +0.6
592,345 + AZOBR_RS32220 0.53 -0.4
592,345 + AZOBR_RS32220 0.53 -0.5
592,346 - AZOBR_RS32220 0.53 +0.2
592,346 - AZOBR_RS32220 0.53 -1.6
592,348 + AZOBR_RS32220 0.53 -2.5
592,358 - AZOBR_RS32220 0.54 +1.9
592,366 + AZOBR_RS32220 0.54 -1.4
592,367 - AZOBR_RS32220 0.55 +0.1

Or see this region's nucleotide sequence