Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS31415 and AZOBR_RS31420 are separated by 285 nucleotides AZOBR_RS31420 and AZOBR_RS31425 are separated by 209 nucleotides AZOBR_RS31425 and AZOBR_RS31430 are separated by 151 nucleotides
AZOBR_RS31415: AZOBR_RS31415 - GntR family transcriptional regulator, at 415,601 to 416,383
_RS31415
AZOBR_RS31420: AZOBR_RS31420 - methylglyoxal synthase, at 416,669 to 417,103
_RS31420
AZOBR_RS31425: AZOBR_RS31425 - glucuronate isomerase, at 417,313 to 418,716
_RS31425
AZOBR_RS31430: AZOBR_RS31430 - galactarate dehydratase, at 418,868 to 420,391
_RS31430
Position (kb) 417
418
419 Strain fitness (log2 ratio) -2
-1
0
1 at 416.507 kb on + strand at 416.509 kb on + strand at 416.509 kb on + strand at 416.509 kb on + strand at 416.510 kb on - strand at 416.510 kb on - strand at 416.510 kb on - strand at 416.513 kb on - strand at 416.513 kb on - strand at 416.513 kb on - strand at 416.513 kb on - strand at 416.610 kb on + strand at 416.619 kb on - strand at 416.981 kb on + strand, within AZOBR_RS31420 at 416.981 kb on + strand, within AZOBR_RS31420 at 416.982 kb on - strand, within AZOBR_RS31420 at 417.204 kb on - strand at 417.494 kb on - strand, within AZOBR_RS31425 at 417.511 kb on + strand, within AZOBR_RS31425 at 417.512 kb on - strand, within AZOBR_RS31425 at 417.512 kb on - strand, within AZOBR_RS31425 at 417.512 kb on - strand, within AZOBR_RS31425 at 417.512 kb on - strand, within AZOBR_RS31425 at 418.015 kb on + strand, within AZOBR_RS31425 at 418.309 kb on + strand, within AZOBR_RS31425 at 418.310 kb on - strand, within AZOBR_RS31425 at 418.705 kb on + strand at 418.706 kb on - strand at 418.706 kb on - strand at 418.706 kb on - strand at 418.820 kb on - strand at 419.039 kb on + strand, within AZOBR_RS31430 at 419.039 kb on + strand, within AZOBR_RS31430 at 419.040 kb on - strand, within AZOBR_RS31430 at 419.040 kb on - strand, within AZOBR_RS31430 at 419.040 kb on - strand, within AZOBR_RS31430 at 419.135 kb on + strand, within AZOBR_RS31430 at 419.240 kb on + strand, within AZOBR_RS31430 at 419.240 kb on + strand, within AZOBR_RS31430 at 419.241 kb on - strand, within AZOBR_RS31430 at 419.241 kb on - strand, within AZOBR_RS31430 at 419.366 kb on + strand, within AZOBR_RS31430 at 419.367 kb on - strand, within AZOBR_RS31430 at 419.367 kb on - strand, within AZOBR_RS31430 at 419.699 kb on + strand, within AZOBR_RS31430 at 419.700 kb on - strand, within AZOBR_RS31430
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 416,507 + +0.6 416,509 + -1.7 416,509 + -0.2 416,509 + +1.6 416,510 - -0.2 416,510 - +0.2 416,510 - -0.0 416,513 - +0.9 416,513 - -2.3 416,513 - -0.4 416,513 - +0.1 416,610 + +0.2 416,619 - -0.9 416,981 + AZOBR_RS31420 0.72 +0.3 416,981 + AZOBR_RS31420 0.72 +1.0 416,982 - AZOBR_RS31420 0.72 +1.1 417,204 - +1.2 417,494 - AZOBR_RS31425 0.13 -0.2 417,511 + AZOBR_RS31425 0.14 -0.4 417,512 - AZOBR_RS31425 0.14 +0.0 417,512 - AZOBR_RS31425 0.14 +1.2 417,512 - AZOBR_RS31425 0.14 +0.8 417,512 - AZOBR_RS31425 0.14 +0.1 418,015 + AZOBR_RS31425 0.50 -0.6 418,309 + AZOBR_RS31425 0.71 -0.3 418,310 - AZOBR_RS31425 0.71 +0.4 418,705 + -1.8 418,706 - +1.2 418,706 - -0.9 418,706 - -0.8 418,820 - +0.3 419,039 + AZOBR_RS31430 0.11 -2.3 419,039 + AZOBR_RS31430 0.11 +1.5 419,040 - AZOBR_RS31430 0.11 -0.3 419,040 - AZOBR_RS31430 0.11 +0.1 419,040 - AZOBR_RS31430 0.11 -0.2 419,135 + AZOBR_RS31430 0.18 -1.9 419,240 + AZOBR_RS31430 0.24 -0.0 419,240 + AZOBR_RS31430 0.24 +1.3 419,241 - AZOBR_RS31430 0.24 -2.1 419,241 - AZOBR_RS31430 0.24 +0.1 419,366 + AZOBR_RS31430 0.33 +0.2 419,367 - AZOBR_RS31430 0.33 -0.2 419,367 - AZOBR_RS31430 0.33 -0.5 419,699 + AZOBR_RS31430 0.55 +0.3 419,700 - AZOBR_RS31430 0.55 +1.1
Or see this region's nucleotide sequence