Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS31240 and AZOBR_RS31245 are separated by 255 nucleotides AZOBR_RS31245 and AZOBR_RS31250 overlap by 4 nucleotides
AZOBR_RS31240: AZOBR_RS31240 - sugar ABC transporter substrate-binding protein, at 373,056 to 374,132
_RS31240
AZOBR_RS31245: AZOBR_RS31245 - ABC transporter ATP-binding protein, at 374,388 to 375,944
_RS31245
AZOBR_RS31250: AZOBR_RS31250 - ABC transporter permease, at 375,941 to 377,152
_RS31250
Position (kb) 374
375
376 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 373.443 kb on + strand, within AZOBR_RS31240 at 373.443 kb on + strand, within AZOBR_RS31240 at 373.443 kb on + strand, within AZOBR_RS31240 at 373.443 kb on + strand, within AZOBR_RS31240 at 373.446 kb on + strand, within AZOBR_RS31240 at 373.446 kb on + strand, within AZOBR_RS31240 at 373.491 kb on + strand, within AZOBR_RS31240 at 373.491 kb on + strand, within AZOBR_RS31240 at 373.491 kb on + strand, within AZOBR_RS31240 at 373.491 kb on + strand, within AZOBR_RS31240 at 373.492 kb on - strand, within AZOBR_RS31240 at 373.492 kb on - strand, within AZOBR_RS31240 at 373.578 kb on + strand, within AZOBR_RS31240 at 373.579 kb on - strand, within AZOBR_RS31240 at 373.587 kb on + strand, within AZOBR_RS31240 at 373.588 kb on - strand, within AZOBR_RS31240 at 373.737 kb on + strand, within AZOBR_RS31240 at 374.043 kb on + strand at 374.106 kb on + strand at 374.107 kb on - strand at 374.109 kb on + strand at 374.109 kb on + strand at 374.109 kb on + strand at 374.109 kb on + strand at 374.109 kb on + strand at 374.110 kb on - strand at 374.110 kb on - strand at 374.168 kb on - strand at 374.168 kb on - strand at 374.262 kb on + strand at 374.405 kb on - strand at 374.550 kb on + strand, within AZOBR_RS31245 at 375.720 kb on + strand, within AZOBR_RS31245 at 375.720 kb on + strand, within AZOBR_RS31245 at 375.721 kb on - strand, within AZOBR_RS31245 at 375.729 kb on + strand, within AZOBR_RS31245 at 375.729 kb on + strand, within AZOBR_RS31245 at 375.729 kb on + strand, within AZOBR_RS31245 at 375.730 kb on - strand, within AZOBR_RS31245 at 375.730 kb on - strand, within AZOBR_RS31245 at 375.822 kb on + strand at 375.823 kb on - strand at 375.823 kb on - strand at 375.823 kb on - strand at 376.065 kb on - strand, within AZOBR_RS31250 at 376.065 kb on - strand, within AZOBR_RS31250 at 376.127 kb on + strand, within AZOBR_RS31250 at 376.127 kb on + strand, within AZOBR_RS31250 at 376.127 kb on + strand, within AZOBR_RS31250 at 376.128 kb on - strand, within AZOBR_RS31250 at 376.128 kb on - strand, within AZOBR_RS31250 at 376.128 kb on - strand, within AZOBR_RS31250 at 376.128 kb on - strand, within AZOBR_RS31250 at 376.128 kb on - strand, within AZOBR_RS31250 at 376.128 kb on - strand, within AZOBR_RS31250 at 376.658 kb on + strand, within AZOBR_RS31250 at 376.658 kb on + strand, within AZOBR_RS31250 at 376.658 kb on + strand, within AZOBR_RS31250 at 376.658 kb on + strand, within AZOBR_RS31250 at 376.658 kb on + strand, within AZOBR_RS31250 at 376.659 kb on - strand at 376.659 kb on - strand, within AZOBR_RS31250 at 376.659 kb on - strand, within AZOBR_RS31250 at 376.733 kb on + strand, within AZOBR_RS31250 at 376.734 kb on - strand, within AZOBR_RS31250 at 376.734 kb on - strand, within AZOBR_RS31250 at 376.841 kb on + strand, within AZOBR_RS31250
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 373,443 + AZOBR_RS31240 0.36 +0.6 373,443 + AZOBR_RS31240 0.36 -0.8 373,443 + AZOBR_RS31240 0.36 -0.2 373,443 + AZOBR_RS31240 0.36 +0.5 373,446 + AZOBR_RS31240 0.36 +1.1 373,446 + AZOBR_RS31240 0.36 -0.8 373,491 + AZOBR_RS31240 0.40 -0.5 373,491 + AZOBR_RS31240 0.40 -0.9 373,491 + AZOBR_RS31240 0.40 +0.6 373,491 + AZOBR_RS31240 0.40 -2.6 373,492 - AZOBR_RS31240 0.40 -2.4 373,492 - AZOBR_RS31240 0.40 -0.1 373,578 + AZOBR_RS31240 0.48 -0.8 373,579 - AZOBR_RS31240 0.49 -1.4 373,587 + AZOBR_RS31240 0.49 -1.2 373,588 - AZOBR_RS31240 0.49 -0.8 373,737 + AZOBR_RS31240 0.63 +1.6 374,043 + +1.0 374,106 + -0.6 374,107 - +0.1 374,109 + +2.2 374,109 + +0.2 374,109 + -1.6 374,109 + -1.9 374,109 + -0.1 374,110 - -1.5 374,110 - +0.2 374,168 - -1.7 374,168 - -0.4 374,262 + +0.5 374,405 - -0.9 374,550 + AZOBR_RS31245 0.10 -0.1 375,720 + AZOBR_RS31245 0.86 -0.3 375,720 + AZOBR_RS31245 0.86 -0.9 375,721 - AZOBR_RS31245 0.86 -2.5 375,729 + AZOBR_RS31245 0.86 -1.8 375,729 + AZOBR_RS31245 0.86 -0.0 375,729 + AZOBR_RS31245 0.86 +0.7 375,730 - AZOBR_RS31245 0.86 -2.1 375,730 - AZOBR_RS31245 0.86 -0.2 375,822 + -2.1 375,823 - -1.5 375,823 - -0.2 375,823 - -0.2 376,065 - AZOBR_RS31250 0.10 +0.4 376,065 - AZOBR_RS31250 0.10 +0.4 376,127 + AZOBR_RS31250 0.15 -0.7 376,127 + AZOBR_RS31250 0.15 +0.4 376,127 + AZOBR_RS31250 0.15 +0.6 376,128 - AZOBR_RS31250 0.15 -4.5 376,128 - AZOBR_RS31250 0.15 +0.9 376,128 - AZOBR_RS31250 0.15 -0.6 376,128 - AZOBR_RS31250 0.15 +0.7 376,128 - AZOBR_RS31250 0.15 +0.0 376,128 - AZOBR_RS31250 0.15 -3.1 376,658 + AZOBR_RS31250 0.59 -0.9 376,658 + AZOBR_RS31250 0.59 +0.1 376,658 + AZOBR_RS31250 0.59 -0.1 376,658 + AZOBR_RS31250 0.59 -0.2 376,658 + AZOBR_RS31250 0.59 +1.1 376,659 - +0.4 376,659 - AZOBR_RS31250 0.59 -0.9 376,659 - AZOBR_RS31250 0.59 +0.9 376,733 + AZOBR_RS31250 0.65 -0.1 376,734 - AZOBR_RS31250 0.65 -1.8 376,734 - AZOBR_RS31250 0.65 +0.1 376,841 + AZOBR_RS31250 0.74 +0.4
Or see this region's nucleotide sequence