Strain Fitness in Azospirillum brasilense Sp245 around AZOBR_RS29955

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAZOBR_RS29945 and AZOBR_RS29950 are separated by 1 nucleotidesAZOBR_RS29950 and AZOBR_RS29955 are separated by 70 nucleotidesAZOBR_RS29955 and AZOBR_RS29960 are separated by 10 nucleotidesAZOBR_RS29960 and AZOBR_RS29965 are separated by 24 nucleotides AZOBR_RS29945: AZOBR_RS29945 - flagellar biosynthesis protein FliQ, at 59,941 to 60,207 _RS29945 AZOBR_RS29950: AZOBR_RS29950 - transglycosylase, at 60,209 to 60,979 _RS29950 AZOBR_RS29955: AZOBR_RS29955 - flagellar biosynthesis protein FlhA, at 61,050 to 63,143 _RS29955 AZOBR_RS29960: AZOBR_RS29960 - flagellar biosynthesis protein, at 63,154 to 63,912 _RS29960 AZOBR_RS29965: AZOBR_RS29965 - flagellar biosynthesis protein, at 63,937 to 65,037 _RS29965 Position (kb) 61 62 63 64Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 60.148 kb on + strand, within AZOBR_RS29945at 60.148 kb on + strand, within AZOBR_RS29945at 60.149 kb on - strand, within AZOBR_RS29945at 60.149 kb on - strand, within AZOBR_RS29945at 60.166 kb on + strand, within AZOBR_RS29945at 60.167 kb on - strand, within AZOBR_RS29945at 60.512 kb on + strand, within AZOBR_RS29950at 60.513 kb on - strand, within AZOBR_RS29950at 60.607 kb on + strand, within AZOBR_RS29950at 60.607 kb on + strand, within AZOBR_RS29950at 60.608 kb on - strand, within AZOBR_RS29950at 60.608 kb on - strand, within AZOBR_RS29950at 60.608 kb on - strand, within AZOBR_RS29950at 60.608 kb on - strand, within AZOBR_RS29950at 60.608 kb on - strand, within AZOBR_RS29950at 60.608 kb on - strand, within AZOBR_RS29950at 60.608 kb on - strand, within AZOBR_RS29950at 60.608 kb on - strand, within AZOBR_RS29950at 60.737 kb on + strand, within AZOBR_RS29950at 60.935 kb on + strandat 60.989 kb on - strandat 60.989 kb on - strandat 60.989 kb on - strandat 61.019 kb on + strandat 61.779 kb on + strand, within AZOBR_RS29955at 61.779 kb on + strand, within AZOBR_RS29955at 61.780 kb on - strand, within AZOBR_RS29955at 61.780 kb on - strand, within AZOBR_RS29955at 62.307 kb on + strand, within AZOBR_RS29955at 62.308 kb on - strand, within AZOBR_RS29955at 62.308 kb on - strand, within AZOBR_RS29955at 62.308 kb on - strand, within AZOBR_RS29955at 62.308 kb on - strand, within AZOBR_RS29955at 62.308 kb on - strand, within AZOBR_RS29955at 62.308 kb on - strand, within AZOBR_RS29955at 62.617 kb on - strand, within AZOBR_RS29955at 62.654 kb on + strand, within AZOBR_RS29955at 62.655 kb on - strand, within AZOBR_RS29955at 62.655 kb on - strand, within AZOBR_RS29955at 62.655 kb on - strand, within AZOBR_RS29955at 63.191 kb on - strandat 63.587 kb on - strand, within AZOBR_RS29960at 63.616 kb on + strand, within AZOBR_RS29960at 63.616 kb on + strand, within AZOBR_RS29960at 63.617 kb on - strand, within AZOBR_RS29960at 63.671 kb on - strand, within AZOBR_RS29960at 63.786 kb on + strand, within AZOBR_RS29960at 63.787 kb on - strand, within AZOBR_RS29960at 63.787 kb on - strand, within AZOBR_RS29960at 63.787 kb on - strand, within AZOBR_RS29960at 63.787 kb on - strand, within AZOBR_RS29960at 63.796 kb on + strand, within AZOBR_RS29960at 63.797 kb on - strand, within AZOBR_RS29960at 63.797 kb on - strand, within AZOBR_RS29960

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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60,148 + AZOBR_RS29945 0.78 +0.8
60,148 + AZOBR_RS29945 0.78 +1.0
60,149 - AZOBR_RS29945 0.78 +1.5
60,149 - AZOBR_RS29945 0.78 -1.9
60,166 + AZOBR_RS29945 0.84 +2.2
60,167 - AZOBR_RS29945 0.85 +0.2
60,512 + AZOBR_RS29950 0.39 -0.2
60,513 - AZOBR_RS29950 0.39 +0.6
60,607 + AZOBR_RS29950 0.52 -0.4
60,607 + AZOBR_RS29950 0.52 +0.8
60,608 - AZOBR_RS29950 0.52 +0.3
60,608 - AZOBR_RS29950 0.52 +1.2
60,608 - AZOBR_RS29950 0.52 +1.1
60,608 - AZOBR_RS29950 0.52 +0.7
60,608 - AZOBR_RS29950 0.52 -0.3
60,608 - AZOBR_RS29950 0.52 +0.6
60,608 - AZOBR_RS29950 0.52 +1.9
60,608 - AZOBR_RS29950 0.52 -0.3
60,737 + AZOBR_RS29950 0.68 +0.3
60,935 + +0.8
60,989 - +0.8
60,989 - +0.6
60,989 - -0.3
61,019 + -1.5
61,779 + AZOBR_RS29955 0.35 -0.7
61,779 + AZOBR_RS29955 0.35 -2.8
61,780 - AZOBR_RS29955 0.35 +0.7
61,780 - AZOBR_RS29955 0.35 -0.2
62,307 + AZOBR_RS29955 0.60 +1.1
62,308 - AZOBR_RS29955 0.60 -1.6
62,308 - AZOBR_RS29955 0.60 +1.6
62,308 - AZOBR_RS29955 0.60 +2.0
62,308 - AZOBR_RS29955 0.60 -0.2
62,308 - AZOBR_RS29955 0.60 -2.1
62,308 - AZOBR_RS29955 0.60 +1.2
62,617 - AZOBR_RS29955 0.75 +0.6
62,654 + AZOBR_RS29955 0.77 +2.3
62,655 - AZOBR_RS29955 0.77 +0.7
62,655 - AZOBR_RS29955 0.77 +0.3
62,655 - AZOBR_RS29955 0.77 +1.2
63,191 - +1.6
63,587 - AZOBR_RS29960 0.57 -1.2
63,616 + AZOBR_RS29960 0.61 +0.5
63,616 + AZOBR_RS29960 0.61 -0.6
63,617 - AZOBR_RS29960 0.61 -0.8
63,671 - AZOBR_RS29960 0.68 +0.5
63,786 + AZOBR_RS29960 0.83 +0.1
63,787 - AZOBR_RS29960 0.83 +0.9
63,787 - AZOBR_RS29960 0.83 +1.6
63,787 - AZOBR_RS29960 0.83 +0.4
63,787 - AZOBR_RS29960 0.83 +2.6
63,796 + AZOBR_RS29960 0.85 -0.2
63,797 - AZOBR_RS29960 0.85 -0.8
63,797 - AZOBR_RS29960 0.85 -3.3

Or see this region's nucleotide sequence