Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS29840 and AZOBR_RS29845 are separated by 287 nucleotides AZOBR_RS29845 and AZOBR_RS29850 are separated by 5 nucleotides AZOBR_RS29850 and AZOBR_RS29855 overlap by 4 nucleotides
AZOBR_RS29840: AZOBR_RS29840 - sulfoxide reductase catalytic subunit YedY, at 41,845 to 42,792
_RS29840
AZOBR_RS29845: AZOBR_RS29845 - oxidoreductase, at 43,080 to 44,108
_RS29845
AZOBR_RS29850: AZOBR_RS29850 - ketohydroxyglutarate aldolase, at 44,114 to 44,749
_RS29850
AZOBR_RS29855: AZOBR_RS29855 - GntR family transcriptional regulator, at 44,746 to 45,513
_RS29855
Position (kb) 43
44
45 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 42.102 kb on - strand, within AZOBR_RS29840 at 42.102 kb on - strand, within AZOBR_RS29840 at 42.161 kb on + strand, within AZOBR_RS29840 at 42.161 kb on + strand, within AZOBR_RS29840 at 42.161 kb on + strand, within AZOBR_RS29840 at 42.161 kb on + strand, within AZOBR_RS29840 at 42.161 kb on + strand, within AZOBR_RS29840 at 42.296 kb on + strand, within AZOBR_RS29840 at 42.296 kb on + strand, within AZOBR_RS29840 at 42.296 kb on + strand, within AZOBR_RS29840 at 42.297 kb on - strand, within AZOBR_RS29840 at 42.297 kb on - strand, within AZOBR_RS29840 at 42.297 kb on - strand, within AZOBR_RS29840 at 42.399 kb on - strand, within AZOBR_RS29840 at 42.848 kb on + strand at 42.848 kb on + strand at 42.849 kb on - strand at 42.849 kb on - strand at 43.072 kb on + strand at 43.072 kb on + strand at 43.072 kb on + strand at 43.072 kb on + strand at 43.073 kb on - strand at 43.073 kb on - strand at 43.546 kb on + strand, within AZOBR_RS29845 at 43.546 kb on + strand, within AZOBR_RS29845 at 43.547 kb on - strand, within AZOBR_RS29845 at 43.547 kb on - strand, within AZOBR_RS29845 at 43.547 kb on - strand, within AZOBR_RS29845 at 43.547 kb on - strand, within AZOBR_RS29845 at 43.627 kb on + strand, within AZOBR_RS29845 at 43.627 kb on + strand, within AZOBR_RS29845 at 43.628 kb on - strand, within AZOBR_RS29845 at 43.628 kb on - strand, within AZOBR_RS29845 at 43.630 kb on + strand, within AZOBR_RS29845 at 43.630 kb on + strand, within AZOBR_RS29845 at 43.631 kb on - strand, within AZOBR_RS29845 at 43.631 kb on - strand, within AZOBR_RS29845 at 43.631 kb on - strand, within AZOBR_RS29845 at 44.115 kb on + strand at 44.115 kb on + strand at 44.425 kb on - strand, within AZOBR_RS29850 at 44.774 kb on + strand at 44.774 kb on + strand at 44.774 kb on + strand at 44.775 kb on - strand at 44.775 kb on - strand at 44.775 kb on - strand at 44.775 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 42,102 - AZOBR_RS29840 0.27 -0.1 42,102 - AZOBR_RS29840 0.27 +0.7 42,161 + AZOBR_RS29840 0.33 -1.7 42,161 + AZOBR_RS29840 0.33 -0.9 42,161 + AZOBR_RS29840 0.33 +0.1 42,161 + AZOBR_RS29840 0.33 +0.0 42,161 + AZOBR_RS29840 0.33 +0.1 42,296 + AZOBR_RS29840 0.48 +0.5 42,296 + AZOBR_RS29840 0.48 -1.4 42,296 + AZOBR_RS29840 0.48 +1.0 42,297 - AZOBR_RS29840 0.48 +1.0 42,297 - AZOBR_RS29840 0.48 -3.1 42,297 - AZOBR_RS29840 0.48 +0.6 42,399 - AZOBR_RS29840 0.58 -0.5 42,848 + +0.3 42,848 + +1.5 42,849 - +1.0 42,849 - +0.4 43,072 + +0.6 43,072 + -1.0 43,072 + -2.2 43,072 + -0.1 43,073 - -0.5 43,073 - +3.3 43,546 + AZOBR_RS29845 0.45 -2.0 43,546 + AZOBR_RS29845 0.45 -3.5 43,547 - AZOBR_RS29845 0.45 -0.8 43,547 - AZOBR_RS29845 0.45 -1.8 43,547 - AZOBR_RS29845 0.45 +0.7 43,547 - AZOBR_RS29845 0.45 -0.9 43,627 + AZOBR_RS29845 0.53 +1.0 43,627 + AZOBR_RS29845 0.53 -0.5 43,628 - AZOBR_RS29845 0.53 +0.7 43,628 - AZOBR_RS29845 0.53 -0.6 43,630 + AZOBR_RS29845 0.53 +0.6 43,630 + AZOBR_RS29845 0.53 -0.3 43,631 - AZOBR_RS29845 0.54 -0.4 43,631 - AZOBR_RS29845 0.54 -1.4 43,631 - AZOBR_RS29845 0.54 +0.1 44,115 + -1.2 44,115 + -0.4 44,425 - AZOBR_RS29850 0.49 +2.1 44,774 + +0.8 44,774 + +0.4 44,774 + +0.9 44,775 - -0.1 44,775 - -0.0 44,775 - -0.2 44,775 - -0.1
Or see this region's nucleotide sequence