Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS27480 and AZOBR_RS27485 are separated by 3 nucleotides AZOBR_RS27485 and AZOBR_RS27490 are separated by 234 nucleotides AZOBR_RS27490 and AZOBR_RS27495 are separated by 53 nucleotides
AZOBR_RS27480: AZOBR_RS27480 - hypothetical protein, at 318,005 to 319,006
_RS27480
AZOBR_RS27485: AZOBR_RS27485 - oxidoreductase, at 319,010 to 319,630
_RS27485
AZOBR_RS27490: AZOBR_RS27490 - hypothetical protein, at 319,865 to 320,362
_RS27490
AZOBR_RS27495: AZOBR_RS27495 - short-chain dehydrogenase, at 320,416 to 321,249
_RS27495
Position (kb) 319
320 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 318.285 kb on + strand, within AZOBR_RS27480 at 318.312 kb on + strand, within AZOBR_RS27480 at 318.313 kb on - strand, within AZOBR_RS27480 at 318.316 kb on + strand, within AZOBR_RS27480 at 318.317 kb on - strand, within AZOBR_RS27480 at 318.317 kb on - strand, within AZOBR_RS27480 at 318.616 kb on - strand, within AZOBR_RS27480 at 318.643 kb on - strand, within AZOBR_RS27480 at 318.900 kb on + strand, within AZOBR_RS27480 at 318.901 kb on - strand, within AZOBR_RS27480 at 318.924 kb on + strand at 318.925 kb on - strand at 318.990 kb on + strand at 318.990 kb on + strand at 318.991 kb on - strand at 319.098 kb on + strand, within AZOBR_RS27485 at 319.098 kb on + strand, within AZOBR_RS27485 at 319.099 kb on - strand, within AZOBR_RS27485 at 319.180 kb on - strand, within AZOBR_RS27485 at 319.733 kb on + strand at 319.733 kb on + strand at 319.760 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 318,285 + AZOBR_RS27480 0.28 +0.5 318,312 + AZOBR_RS27480 0.31 -2.7 318,313 - AZOBR_RS27480 0.31 -1.9 318,316 + AZOBR_RS27480 0.31 +0.1 318,317 - AZOBR_RS27480 0.31 -0.3 318,317 - AZOBR_RS27480 0.31 -0.9 318,616 - AZOBR_RS27480 0.61 -1.0 318,643 - AZOBR_RS27480 0.64 -1.4 318,900 + AZOBR_RS27480 0.89 -0.8 318,901 - AZOBR_RS27480 0.89 -0.6 318,924 + -1.1 318,925 - +1.0 318,990 + +0.9 318,990 + -2.8 318,991 - +0.9 319,098 + AZOBR_RS27485 0.14 -0.4 319,098 + AZOBR_RS27485 0.14 +1.0 319,099 - AZOBR_RS27485 0.14 +0.1 319,180 - AZOBR_RS27485 0.27 +0.3 319,733 + +0.6 319,733 + -0.9 319,760 + +1.3
Or see this region's nucleotide sequence