Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS18355 and AZOBR_RS18360 are separated by 2 nucleotides AZOBR_RS18360 and AZOBR_RS18365 are separated by 70 nucleotides AZOBR_RS18365 and AZOBR_RS18370 are separated by 181 nucleotides
AZOBR_RS18355: AZOBR_RS18355 - endonuclease, at 884,021 to 884,995
_RS18355
AZOBR_RS18360: AZOBR_RS18360 - hypothetical protein, at 884,998 to 885,576
_RS18360
AZOBR_RS18365: AZOBR_RS18365 - hypothetical protein, at 885,647 to 885,889
_RS18365
AZOBR_RS18370: AZOBR_RS18370 - aspartate decarboxylase, at 886,071 to 886,505
_RS18370
Position (kb)
884
885
886 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 884.576 kb on + strand, within AZOBR_RS18355 at 884.576 kb on + strand, within AZOBR_RS18355 at 884.960 kb on + strand at 885.041 kb on + strand at 885.281 kb on + strand, within AZOBR_RS18360 at 885.347 kb on + strand, within AZOBR_RS18360 at 885.347 kb on + strand, within AZOBR_RS18360 at 885.347 kb on + strand, within AZOBR_RS18360 at 885.348 kb on - strand, within AZOBR_RS18360 at 885.348 kb on - strand, within AZOBR_RS18360 at 885.378 kb on - strand, within AZOBR_RS18360 at 885.378 kb on - strand, within AZOBR_RS18360 at 885.378 kb on - strand, within AZOBR_RS18360 at 885.399 kb on - strand, within AZOBR_RS18360 at 885.837 kb on - strand, within AZOBR_RS18365 at 885.865 kb on - strand at 885.873 kb on - strand at 885.873 kb on - strand at 885.926 kb on + strand at 885.927 kb on - strand at 886.150 kb on + strand, within AZOBR_RS18370 at 886.235 kb on - strand, within AZOBR_RS18370 at 886.327 kb on + strand, within AZOBR_RS18370 at 886.327 kb on + strand, within AZOBR_RS18370 at 886.328 kb on - strand, within AZOBR_RS18370 at 886.328 kb on - strand, within AZOBR_RS18370 at 886.336 kb on + strand, within AZOBR_RS18370 at 886.336 kb on + strand, within AZOBR_RS18370 at 886.336 kb on + strand, within AZOBR_RS18370 at 886.336 kb on + strand, within AZOBR_RS18370 at 886.336 kb on + strand, within AZOBR_RS18370 at 886.336 kb on + strand, within AZOBR_RS18370 at 886.337 kb on - strand, within AZOBR_RS18370 at 886.337 kb on - strand, within AZOBR_RS18370 at 886.337 kb on - strand, within AZOBR_RS18370 at 886.337 kb on - strand, within AZOBR_RS18370 at 886.337 kb on - strand, within AZOBR_RS18370 at 886.337 kb on - strand, within AZOBR_RS18370 at 886.337 kb on - strand, within AZOBR_RS18370 at 886.337 kb on - strand, within AZOBR_RS18370 at 886.337 kb on - strand, within AZOBR_RS18370 at 886.337 kb on - strand, within AZOBR_RS18370 at 886.337 kb on - strand, within AZOBR_RS18370 at 886.337 kb on - strand, within AZOBR_RS18370 at 886.393 kb on + strand, within AZOBR_RS18370 at 886.393 kb on + strand, within AZOBR_RS18370 at 886.393 kb on + strand, within AZOBR_RS18370 at 886.394 kb on - strand, within AZOBR_RS18370 at 886.444 kb on + strand, within AZOBR_RS18370 at 886.444 kb on + strand, within AZOBR_RS18370 at 886.444 kb on + strand, within AZOBR_RS18370 at 886.444 kb on + strand, within AZOBR_RS18370 at 886.445 kb on - strand, within AZOBR_RS18370 at 886.445 kb on - strand, within AZOBR_RS18370 at 886.485 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 884,576 + AZOBR_RS18355 0.57 +1.1 884,576 + AZOBR_RS18355 0.57 -0.2 884,960 + -3.6 885,041 + +0.5 885,281 + AZOBR_RS18360 0.49 -0.5 885,347 + AZOBR_RS18360 0.60 -2.6 885,347 + AZOBR_RS18360 0.60 +0.9 885,347 + AZOBR_RS18360 0.60 +0.4 885,348 - AZOBR_RS18360 0.60 +0.5 885,348 - AZOBR_RS18360 0.60 +0.2 885,378 - AZOBR_RS18360 0.66 +0.3 885,378 - AZOBR_RS18360 0.66 +0.4 885,378 - AZOBR_RS18360 0.66 +0.3 885,399 - AZOBR_RS18360 0.69 +1.0 885,837 - AZOBR_RS18365 0.78 -1.4 885,865 - +0.4 885,873 - -0.4 885,873 - +0.2 885,926 + -0.0 885,927 - +0.1 886,150 + AZOBR_RS18370 0.18 -1.0 886,235 - AZOBR_RS18370 0.38 -0.1 886,327 + AZOBR_RS18370 0.59 -0.2 886,327 + AZOBR_RS18370 0.59 +1.1 886,328 - AZOBR_RS18370 0.59 +0.4 886,328 - AZOBR_RS18370 0.59 +0.6 886,336 + AZOBR_RS18370 0.61 +0.4 886,336 + AZOBR_RS18370 0.61 -0.3 886,336 + AZOBR_RS18370 0.61 -2.7 886,336 + AZOBR_RS18370 0.61 +0.6 886,336 + AZOBR_RS18370 0.61 +3.1 886,336 + AZOBR_RS18370 0.61 +0.8 886,337 - AZOBR_RS18370 0.61 +0.9 886,337 - AZOBR_RS18370 0.61 +1.9 886,337 - AZOBR_RS18370 0.61 +0.1 886,337 - AZOBR_RS18370 0.61 -0.4 886,337 - AZOBR_RS18370 0.61 +1.9 886,337 - AZOBR_RS18370 0.61 +0.6 886,337 - AZOBR_RS18370 0.61 +1.1 886,337 - AZOBR_RS18370 0.61 -0.1 886,337 - AZOBR_RS18370 0.61 -0.3 886,337 - AZOBR_RS18370 0.61 +1.3 886,337 - AZOBR_RS18370 0.61 -2.0 886,337 - AZOBR_RS18370 0.61 -2.4 886,393 + AZOBR_RS18370 0.74 +0.8 886,393 + AZOBR_RS18370 0.74 +1.1 886,393 + AZOBR_RS18370 0.74 -1.1 886,394 - AZOBR_RS18370 0.74 -0.1 886,444 + AZOBR_RS18370 0.86 -2.3 886,444 + AZOBR_RS18370 0.86 +1.0 886,444 + AZOBR_RS18370 0.86 +1.0 886,444 + AZOBR_RS18370 0.86 -3.7 886,445 - AZOBR_RS18370 0.86 -0.8 886,445 - AZOBR_RS18370 0.86 +0.2 886,485 - +0.4
Or see this region's nucleotide sequence