Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS13620 and AZOBR_RS13625 are separated by 10 nucleotides AZOBR_RS13625 and AZOBR_RS13630 are separated by 0 nucleotides AZOBR_RS13630 and AZOBR_RS13635 overlap by 4 nucleotides AZOBR_RS13635 and AZOBR_RS13640 are separated by 73 nucleotides
AZOBR_RS13620: AZOBR_RS13620 - hypothetical protein, at 2,890,032 to 2,892,131
_RS13620
AZOBR_RS13625: AZOBR_RS13625 - hypothetical protein, at 2,892,142 to 2,892,759
_RS13625
AZOBR_RS13630: AZOBR_RS13630 - hypothetical protein, at 2,892,760 to 2,893,107
_RS13630
AZOBR_RS13635: AZOBR_RS13635 - hypothetical protein, at 2,893,104 to 2,893,316
_RS13635
AZOBR_RS13640: AZOBR_RS13640 - putative phage Major head protein, at 2,893,390 to 2,894,343
_RS13640
Position (kb)
2893
2894 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2892.278 kb on + strand, within AZOBR_RS13625 at 2892.278 kb on + strand, within AZOBR_RS13625 at 2892.278 kb on + strand, within AZOBR_RS13625 at 2892.279 kb on - strand, within AZOBR_RS13625 at 2892.279 kb on - strand, within AZOBR_RS13625 at 2892.279 kb on - strand, within AZOBR_RS13625 at 2892.312 kb on - strand, within AZOBR_RS13625 at 2892.312 kb on - strand, within AZOBR_RS13625 at 2892.312 kb on - strand, within AZOBR_RS13625 at 2892.312 kb on - strand, within AZOBR_RS13625 at 2892.383 kb on + strand, within AZOBR_RS13625 at 2892.384 kb on - strand, within AZOBR_RS13625 at 2892.384 kb on - strand, within AZOBR_RS13625 at 2892.384 kb on - strand, within AZOBR_RS13625 at 2892.384 kb on - strand, within AZOBR_RS13625 at 2892.384 kb on - strand, within AZOBR_RS13625 at 2892.384 kb on - strand, within AZOBR_RS13625 at 2892.447 kb on + strand, within AZOBR_RS13625 at 2892.447 kb on + strand, within AZOBR_RS13625 at 2892.448 kb on - strand, within AZOBR_RS13625 at 2892.587 kb on + strand, within AZOBR_RS13625 at 2892.742 kb on - strand at 2892.869 kb on + strand, within AZOBR_RS13630 at 2892.956 kb on + strand, within AZOBR_RS13630 at 2892.957 kb on - strand, within AZOBR_RS13630 at 2893.168 kb on + strand, within AZOBR_RS13635 at 2893.168 kb on + strand, within AZOBR_RS13635 at 2893.168 kb on + strand, within AZOBR_RS13635 at 2893.169 kb on - strand, within AZOBR_RS13635 at 2893.169 kb on - strand, within AZOBR_RS13635 at 2893.169 kb on - strand, within AZOBR_RS13635 at 2893.169 kb on - strand, within AZOBR_RS13635 at 2893.169 kb on - strand, within AZOBR_RS13635 at 2893.169 kb on - strand, within AZOBR_RS13635 at 2893.392 kb on - strand at 2893.392 kb on - strand at 2893.613 kb on + strand, within AZOBR_RS13640 at 2893.613 kb on + strand, within AZOBR_RS13640 at 2893.613 kb on + strand, within AZOBR_RS13640 at 2893.613 kb on + strand, within AZOBR_RS13640 at 2893.613 kb on + strand, within AZOBR_RS13640 at 2893.614 kb on - strand, within AZOBR_RS13640 at 2893.614 kb on - strand, within AZOBR_RS13640 at 2893.614 kb on - strand, within AZOBR_RS13640 at 2894.312 kb on + strand at 2894.312 kb on + strand at 2894.312 kb on + strand at 2894.312 kb on + strand at 2894.312 kb on + strand at 2894.312 kb on + strand at 2894.312 kb on + strand at 2894.313 kb on - strand at 2894.313 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,892,278 + AZOBR_RS13625 0.22 +0.2 2,892,278 + AZOBR_RS13625 0.22 -0.2 2,892,278 + AZOBR_RS13625 0.22 -0.7 2,892,279 - AZOBR_RS13625 0.22 -0.6 2,892,279 - AZOBR_RS13625 0.22 -0.5 2,892,279 - AZOBR_RS13625 0.22 -0.1 2,892,312 - AZOBR_RS13625 0.28 -0.1 2,892,312 - AZOBR_RS13625 0.28 -1.0 2,892,312 - AZOBR_RS13625 0.28 -1.1 2,892,312 - AZOBR_RS13625 0.28 -0.3 2,892,383 + AZOBR_RS13625 0.39 -1.0 2,892,384 - AZOBR_RS13625 0.39 -0.9 2,892,384 - AZOBR_RS13625 0.39 -0.3 2,892,384 - AZOBR_RS13625 0.39 +0.7 2,892,384 - AZOBR_RS13625 0.39 -2.9 2,892,384 - AZOBR_RS13625 0.39 -3.6 2,892,384 - AZOBR_RS13625 0.39 -1.8 2,892,447 + AZOBR_RS13625 0.49 -0.4 2,892,447 + AZOBR_RS13625 0.49 +0.7 2,892,448 - AZOBR_RS13625 0.50 -5.0 2,892,587 + AZOBR_RS13625 0.72 -0.8 2,892,742 - -0.1 2,892,869 + AZOBR_RS13630 0.31 -1.0 2,892,956 + AZOBR_RS13630 0.56 -0.1 2,892,957 - AZOBR_RS13630 0.57 -0.8 2,893,168 + AZOBR_RS13635 0.30 +1.5 2,893,168 + AZOBR_RS13635 0.30 -2.0 2,893,168 + AZOBR_RS13635 0.30 -0.8 2,893,169 - AZOBR_RS13635 0.31 -0.2 2,893,169 - AZOBR_RS13635 0.31 -0.6 2,893,169 - AZOBR_RS13635 0.31 +0.4 2,893,169 - AZOBR_RS13635 0.31 -2.9 2,893,169 - AZOBR_RS13635 0.31 -0.9 2,893,169 - AZOBR_RS13635 0.31 -0.5 2,893,392 - +0.0 2,893,392 - -0.5 2,893,613 + AZOBR_RS13640 0.23 -0.8 2,893,613 + AZOBR_RS13640 0.23 -1.1 2,893,613 + AZOBR_RS13640 0.23 -1.5 2,893,613 + AZOBR_RS13640 0.23 -2.6 2,893,613 + AZOBR_RS13640 0.23 -0.3 2,893,614 - AZOBR_RS13640 0.23 +0.5 2,893,614 - AZOBR_RS13640 0.23 +0.5 2,893,614 - AZOBR_RS13640 0.23 -0.4 2,894,312 + -0.4 2,894,312 + +0.1 2,894,312 + -0.4 2,894,312 + -1.2 2,894,312 + +0.1 2,894,312 + +0.5 2,894,312 + -1.1 2,894,313 - -0.7 2,894,313 - -0.4
Or see this region's nucleotide sequence