Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS05820 and AZOBR_RS05825 are separated by 208 nucleotides AZOBR_RS05825 and AZOBR_RS05830 are separated by 77 nucleotides AZOBR_RS05830 and AZOBR_RS05835 are separated by 27 nucleotides
AZOBR_RS05820: AZOBR_RS05820 - alpha/beta hydrolase, at 1,239,247 to 1,239,909
_RS05820
AZOBR_RS05825: AZOBR_RS05825 - serine O-acetyltransferase, at 1,240,118 to 1,240,948
_RS05825
AZOBR_RS05830: AZOBR_RS05830 - Rrf2 family transcriptional regulator, at 1,241,026 to 1,241,508
_RS05830
AZOBR_RS05835: AZOBR_RS05835 - cysteine desulfarase, at 1,241,536 to 1,242,648
_RS05835
Position (kb)
1240
1241 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1239.293 kb on + strand at 1239.294 kb on - strand at 1239.687 kb on - strand, within AZOBR_RS05820 at 1239.687 kb on - strand at 1239.738 kb on - strand, within AZOBR_RS05820 at 1239.933 kb on + strand at 1239.935 kb on + strand at 1239.935 kb on + strand at 1239.936 kb on - strand at 1239.936 kb on - strand at 1239.936 kb on - strand at 1240.055 kb on - strand at 1240.055 kb on - strand at 1240.055 kb on - strand at 1240.056 kb on + strand at 1240.056 kb on + strand at 1240.057 kb on - strand at 1240.057 kb on - strand at 1240.154 kb on + strand at 1240.156 kb on + strand at 1240.156 kb on + strand at 1240.157 kb on - strand at 1240.207 kb on + strand, within AZOBR_RS05825 at 1240.301 kb on + strand, within AZOBR_RS05825 at 1240.475 kb on + strand, within AZOBR_RS05825 at 1240.475 kb on + strand, within AZOBR_RS05825 at 1240.476 kb on - strand, within AZOBR_RS05825 at 1240.476 kb on - strand, within AZOBR_RS05825 at 1240.719 kb on - strand, within AZOBR_RS05825 at 1240.719 kb on - strand, within AZOBR_RS05825 at 1240.785 kb on + strand, within AZOBR_RS05825 at 1240.785 kb on + strand, within AZOBR_RS05825 at 1240.785 kb on + strand, within AZOBR_RS05825 at 1240.851 kb on + strand, within AZOBR_RS05825 at 1240.853 kb on + strand, within AZOBR_RS05825 at 1241.392 kb on + strand, within AZOBR_RS05830 at 1241.392 kb on + strand, within AZOBR_RS05830 at 1241.392 kb on + strand, within AZOBR_RS05830 at 1241.392 kb on + strand, within AZOBR_RS05830
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 1,239,293 + +0.8 1,239,294 - -3.2 1,239,687 - AZOBR_RS05820 0.66 -0.9 1,239,687 - -0.5 1,239,738 - AZOBR_RS05820 0.74 -5.1 1,239,933 + +0.5 1,239,935 + -1.7 1,239,935 + +0.1 1,239,936 - +0.7 1,239,936 - -2.2 1,239,936 - -0.3 1,240,055 - +1.6 1,240,055 - -1.0 1,240,055 - +0.2 1,240,056 + -2.1 1,240,056 + +1.9 1,240,057 - +1.6 1,240,057 - +1.7 1,240,154 + -0.1 1,240,156 + -0.2 1,240,156 + -0.9 1,240,157 - +1.0 1,240,207 + AZOBR_RS05825 0.11 +0.8 1,240,301 + AZOBR_RS05825 0.22 +1.1 1,240,475 + AZOBR_RS05825 0.43 +1.8 1,240,475 + AZOBR_RS05825 0.43 +0.2 1,240,476 - AZOBR_RS05825 0.43 +0.6 1,240,476 - AZOBR_RS05825 0.43 -1.2 1,240,719 - AZOBR_RS05825 0.72 +0.7 1,240,719 - AZOBR_RS05825 0.72 +2.5 1,240,785 + AZOBR_RS05825 0.80 -1.5 1,240,785 + AZOBR_RS05825 0.80 -0.4 1,240,785 + AZOBR_RS05825 0.80 +1.1 1,240,851 + AZOBR_RS05825 0.88 -0.6 1,240,853 + AZOBR_RS05825 0.88 -3.9 1,241,392 + AZOBR_RS05830 0.76 +3.1 1,241,392 + AZOBR_RS05830 0.76 +2.6 1,241,392 + AZOBR_RS05830 0.76 -1.3 1,241,392 + AZOBR_RS05830 0.76 +2.6
Or see this region's nucleotide sequence