Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS05725 and AZOBR_RS05730 are separated by 10 nucleotides AZOBR_RS05730 and AZOBR_RS05735 are separated by 7 nucleotides
AZOBR_RS05725: AZOBR_RS05725 - FAD-binding protein, at 1,212,952 to 1,214,439
_RS05725
AZOBR_RS05730: AZOBR_RS05730 - 2-hydroxy-acid oxidase, at 1,214,450 to 1,215,703
_RS05730
AZOBR_RS05735: AZOBR_RS05735 - 2-hydroxy-acid oxidase, at 1,215,711 to 1,217,057
_RS05735
Position (kb)
1214
1215
1216 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1213.453 kb on + strand, within AZOBR_RS05725 at 1213.550 kb on - strand, within AZOBR_RS05725 at 1213.897 kb on + strand, within AZOBR_RS05725 at 1213.990 kb on + strand, within AZOBR_RS05725 at 1213.990 kb on + strand, within AZOBR_RS05725 at 1213.991 kb on - strand, within AZOBR_RS05725 at 1213.994 kb on - strand, within AZOBR_RS05725 at 1213.994 kb on - strand, within AZOBR_RS05725 at 1213.994 kb on - strand, within AZOBR_RS05725 at 1213.994 kb on - strand, within AZOBR_RS05725 at 1214.131 kb on + strand, within AZOBR_RS05725 at 1214.131 kb on - strand, within AZOBR_RS05725 at 1214.132 kb on - strand, within AZOBR_RS05725 at 1214.132 kb on - strand, within AZOBR_RS05725 at 1214.588 kb on + strand, within AZOBR_RS05730 at 1214.588 kb on + strand, within AZOBR_RS05730 at 1214.627 kb on + strand, within AZOBR_RS05730 at 1214.882 kb on + strand, within AZOBR_RS05730 at 1214.882 kb on + strand, within AZOBR_RS05730 at 1214.883 kb on - strand, within AZOBR_RS05730 at 1214.883 kb on - strand at 1214.883 kb on - strand, within AZOBR_RS05730 at 1214.883 kb on - strand, within AZOBR_RS05730 at 1215.087 kb on - strand, within AZOBR_RS05730 at 1215.309 kb on - strand, within AZOBR_RS05730 at 1215.456 kb on - strand, within AZOBR_RS05730 at 1215.689 kb on + strand at 1215.689 kb on + strand at 1215.689 kb on + strand at 1215.690 kb on - strand at 1215.690 kb on - strand at 1215.690 kb on - strand at 1215.701 kb on + strand at 1215.701 kb on + strand at 1215.702 kb on - strand at 1215.702 kb on - strand at 1215.702 kb on - strand at 1215.702 kb on - strand at 1215.822 kb on + strand at 1215.822 kb on + strand at 1215.822 kb on + strand at 1215.822 kb on + strand at 1215.822 kb on + strand at 1215.823 kb on - strand at 1215.823 kb on - strand at 1215.823 kb on - strand at 1215.823 kb on - strand at 1215.823 kb on - strand at 1215.867 kb on + strand, within AZOBR_RS05735 at 1215.867 kb on + strand, within AZOBR_RS05735 at 1215.867 kb on + strand, within AZOBR_RS05735 at 1215.867 kb on + strand, within AZOBR_RS05735 at 1215.867 kb on + strand, within AZOBR_RS05735 at 1215.868 kb on - strand, within AZOBR_RS05735 at 1215.984 kb on + strand, within AZOBR_RS05735 at 1215.984 kb on + strand, within AZOBR_RS05735 at 1215.984 kb on + strand, within AZOBR_RS05735 at 1215.985 kb on - strand, within AZOBR_RS05735 at 1215.985 kb on - strand, within AZOBR_RS05735 at 1216.528 kb on - strand, within AZOBR_RS05735 at 1216.557 kb on + strand, within AZOBR_RS05735 at 1216.665 kb on + strand, within AZOBR_RS05735
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 1,213,453 + AZOBR_RS05725 0.34 -0.2 1,213,550 - AZOBR_RS05725 0.40 +0.5 1,213,897 + AZOBR_RS05725 0.64 +0.5 1,213,990 + AZOBR_RS05725 0.70 -1.0 1,213,990 + AZOBR_RS05725 0.70 -1.4 1,213,991 - AZOBR_RS05725 0.70 -1.0 1,213,994 - AZOBR_RS05725 0.70 +0.9 1,213,994 - AZOBR_RS05725 0.70 -0.4 1,213,994 - AZOBR_RS05725 0.70 +0.3 1,213,994 - AZOBR_RS05725 0.70 -0.5 1,214,131 + AZOBR_RS05725 0.79 -1.2 1,214,131 - AZOBR_RS05725 0.79 -0.9 1,214,132 - AZOBR_RS05725 0.79 -0.5 1,214,132 - AZOBR_RS05725 0.79 -1.2 1,214,588 + AZOBR_RS05730 0.11 +0.4 1,214,588 + AZOBR_RS05730 0.11 +0.3 1,214,627 + AZOBR_RS05730 0.14 +0.6 1,214,882 + AZOBR_RS05730 0.34 -1.3 1,214,882 + AZOBR_RS05730 0.34 -1.5 1,214,883 - AZOBR_RS05730 0.35 -1.3 1,214,883 - +2.5 1,214,883 - AZOBR_RS05730 0.35 -0.5 1,214,883 - AZOBR_RS05730 0.35 -0.8 1,215,087 - AZOBR_RS05730 0.51 -0.9 1,215,309 - AZOBR_RS05730 0.69 +0.7 1,215,456 - AZOBR_RS05730 0.80 -0.9 1,215,689 + -0.9 1,215,689 + +0.3 1,215,689 + +1.0 1,215,690 - -3.5 1,215,690 - -2.9 1,215,690 - -1.5 1,215,701 + -0.4 1,215,701 + -0.1 1,215,702 - +0.3 1,215,702 - +1.0 1,215,702 - -0.2 1,215,702 - -0.6 1,215,822 + -2.9 1,215,822 + +0.0 1,215,822 + -1.3 1,215,822 + -1.3 1,215,822 + -0.8 1,215,823 - -0.0 1,215,823 - -1.3 1,215,823 - +1.2 1,215,823 - +0.5 1,215,823 - -2.1 1,215,867 + AZOBR_RS05735 0.12 +0.7 1,215,867 + AZOBR_RS05735 0.12 -0.1 1,215,867 + AZOBR_RS05735 0.12 +0.9 1,215,867 + AZOBR_RS05735 0.12 -0.6 1,215,867 + AZOBR_RS05735 0.12 -3.3 1,215,868 - AZOBR_RS05735 0.12 +0.1 1,215,984 + AZOBR_RS05735 0.20 -1.1 1,215,984 + AZOBR_RS05735 0.20 +0.4 1,215,984 + AZOBR_RS05735 0.20 -1.6 1,215,985 - AZOBR_RS05735 0.20 -0.1 1,215,985 - AZOBR_RS05735 0.20 +0.1 1,216,528 - AZOBR_RS05735 0.61 +0.1 1,216,557 + AZOBR_RS05735 0.63 +0.5 1,216,665 + AZOBR_RS05735 0.71 +0.1
Or see this region's nucleotide sequence