Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS04255 and AZOBR_RS04260 are separated by 131 nucleotides AZOBR_RS04260 and AZOBR_RS04265 are separated by 346 nucleotides AZOBR_RS04265 and AZOBR_RS04270 are separated by 16 nucleotides AZOBR_RS04270 and AZOBR_RS04275 are separated by 53 nucleotides
AZOBR_RS04255: AZOBR_RS04255 - hypothetical protein, at 897,750 to 898,715
_RS04255
AZOBR_RS04260: AZOBR_RS04260 - hypothetical protein, at 898,847 to 899,227
_RS04260
AZOBR_RS04265: AZOBR_RS04265 - 2-isopropylmalate synthase, at 899,574 to 901,178
_RS04265
AZOBR_RS04270: AZOBR_RS04270 - RNA methyltransferase, at 901,195 to 901,965
_RS04270
AZOBR_RS04275: AZOBR_RS04275 - response regulator, at 902,019 to 902,732
_RS04275
Position (kb) 899
900
901
902 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 898.576 kb on + strand, within AZOBR_RS04255 at 898.576 kb on + strand, within AZOBR_RS04255 at 898.576 kb on + strand, within AZOBR_RS04255 at 898.576 kb on + strand, within AZOBR_RS04255 at 898.576 kb on + strand, within AZOBR_RS04255 at 898.576 kb on + strand, within AZOBR_RS04255 at 898.576 kb on + strand, within AZOBR_RS04255 at 898.577 kb on - strand, within AZOBR_RS04255 at 898.577 kb on - strand, within AZOBR_RS04255 at 898.577 kb on - strand, within AZOBR_RS04255 at 898.577 kb on - strand, within AZOBR_RS04255 at 898.577 kb on - strand, within AZOBR_RS04255 at 898.577 kb on - strand, within AZOBR_RS04255 at 899.298 kb on + strand at 899.299 kb on - strand at 899.330 kb on + strand at 899.402 kb on - strand at 899.402 kb on - strand at 899.412 kb on + strand at 899.412 kb on + strand at 899.413 kb on - strand at 899.592 kb on + strand at 899.592 kb on + strand at 899.592 kb on + strand at 899.592 kb on + strand at 899.592 kb on + strand at 899.593 kb on - strand at 899.593 kb on - strand at 899.593 kb on - strand at 899.593 kb on - strand at 899.593 kb on - strand at 899.701 kb on - strand at 899.701 kb on - strand at 899.701 kb on - strand at 900.069 kb on + strand, within AZOBR_RS04265 at 900.069 kb on + strand, within AZOBR_RS04265 at 900.522 kb on + strand, within AZOBR_RS04265 at 900.523 kb on - strand, within AZOBR_RS04265 at 900.523 kb on - strand, within AZOBR_RS04265 at 900.696 kb on + strand, within AZOBR_RS04265 at 900.696 kb on + strand, within AZOBR_RS04265 at 900.697 kb on - strand, within AZOBR_RS04265 at 900.697 kb on - strand, within AZOBR_RS04265 at 900.697 kb on - strand, within AZOBR_RS04265 at 900.765 kb on + strand, within AZOBR_RS04265 at 900.766 kb on - strand, within AZOBR_RS04265 at 900.807 kb on + strand, within AZOBR_RS04265 at 900.877 kb on - strand, within AZOBR_RS04265 at 900.951 kb on + strand, within AZOBR_RS04265 at 901.042 kb on + strand at 901.043 kb on - strand at 901.134 kb on + strand at 901.134 kb on + strand at 901.134 kb on + strand at 901.135 kb on - strand at 901.151 kb on + strand at 901.417 kb on + strand, within AZOBR_RS04270 at 901.418 kb on - strand, within AZOBR_RS04270 at 901.694 kb on - strand, within AZOBR_RS04270 at 901.963 kb on + strand at 902.008 kb on + strand at 902.009 kb on - strand at 902.009 kb on - strand at 902.009 kb on - strand at 902.009 kb on - strand at 902.010 kb on + strand at 902.010 kb on + strand at 902.010 kb on + strand at 902.011 kb on - strand at 902.011 kb on - strand at 902.011 kb on - strand at 902.025 kb on + strand at 902.026 kb on - strand at 902.026 kb on - strand at 902.026 kb on - strand at 902.026 kb on - strand at 902.040 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 898,576 + AZOBR_RS04255 0.86 -2.3 898,576 + AZOBR_RS04255 0.86 -1.8 898,576 + AZOBR_RS04255 0.86 -0.8 898,576 + AZOBR_RS04255 0.86 +0.5 898,576 + AZOBR_RS04255 0.86 +1.4 898,576 + AZOBR_RS04255 0.86 -0.6 898,576 + AZOBR_RS04255 0.86 +0.8 898,577 - AZOBR_RS04255 0.86 -0.7 898,577 - AZOBR_RS04255 0.86 +1.1 898,577 - AZOBR_RS04255 0.86 -1.5 898,577 - AZOBR_RS04255 0.86 -2.3 898,577 - AZOBR_RS04255 0.86 +0.5 898,577 - AZOBR_RS04255 0.86 -3.0 899,298 + -2.1 899,299 - +0.2 899,330 + +0.2 899,402 - +0.3 899,402 - -2.0 899,412 + -0.1 899,412 + +0.5 899,413 - +0.3 899,592 + -0.6 899,592 + -0.1 899,592 + -0.2 899,592 + -1.3 899,592 + -0.1 899,593 - +0.4 899,593 - +0.1 899,593 - +1.1 899,593 - -3.3 899,593 - -1.6 899,701 - -0.4 899,701 - -0.1 899,701 - -2.8 900,069 + AZOBR_RS04265 0.31 +0.7 900,069 + AZOBR_RS04265 0.31 +0.2 900,522 + AZOBR_RS04265 0.59 -0.5 900,523 - AZOBR_RS04265 0.59 +0.4 900,523 - AZOBR_RS04265 0.59 +0.9 900,696 + AZOBR_RS04265 0.70 -1.2 900,696 + AZOBR_RS04265 0.70 +0.4 900,697 - AZOBR_RS04265 0.70 -0.1 900,697 - AZOBR_RS04265 0.70 +0.0 900,697 - AZOBR_RS04265 0.70 -1.8 900,765 + AZOBR_RS04265 0.74 +1.6 900,766 - AZOBR_RS04265 0.74 -0.4 900,807 + AZOBR_RS04265 0.77 -0.3 900,877 - AZOBR_RS04265 0.81 -0.2 900,951 + AZOBR_RS04265 0.86 -2.4 901,042 + -1.3 901,043 - +0.2 901,134 + -0.1 901,134 + +0.8 901,134 + -0.1 901,135 - -0.9 901,151 + +0.9 901,417 + AZOBR_RS04270 0.29 -1.0 901,418 - AZOBR_RS04270 0.29 -0.3 901,694 - AZOBR_RS04270 0.65 -2.5 901,963 + -0.6 902,008 + -1.3 902,009 - +0.0 902,009 - -3.3 902,009 - +1.0 902,009 - -1.0 902,010 + +1.0 902,010 + -1.9 902,010 + +0.3 902,011 - +0.7 902,011 - +1.6 902,011 - -2.3 902,025 + -3.6 902,026 - +0.5 902,026 - -0.1 902,026 - +0.5 902,026 - +1.2 902,040 - -0.2
Or see this region's nucleotide sequence