Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS04065 and AZOBR_RS04070 are separated by 95 nucleotides AZOBR_RS04070 and AZOBR_RS04075 are separated by 117 nucleotides AZOBR_RS04075 and AZOBR_RS04080 are separated by 195 nucleotides
AZOBR_RS04065: AZOBR_RS04065 - ribosome-associated protein IOJAP, at 856,244 to 856,585
_RS04065
AZOBR_RS04070: AZOBR_RS04070 - nicotinic acid mononucleotide adenylyltransferase, at 856,681 to 857,298
_RS04070
AZOBR_RS04075: AZOBR_RS04075 - gamma-glutamyl phosphate reductase, at 857,416 to 858,696
_RS04075
AZOBR_RS04080: AZOBR_RS04080 - cytochrome C, at 858,892 to 859,758
_RS04080
Position (kb) 857
858
859 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 856.664 kb on + strand at 856.664 kb on + strand at 856.664 kb on + strand at 857.314 kb on - strand at 857.314 kb on - strand at 857.396 kb on - strand at 857.396 kb on - strand at 857.396 kb on - strand at 857.411 kb on - strand at 858.855 kb on - strand at 859.124 kb on - strand, within AZOBR_RS04080 at 859.297 kb on + strand, within AZOBR_RS04080 at 859.297 kb on + strand, within AZOBR_RS04080 at 859.297 kb on + strand, within AZOBR_RS04080 at 859.298 kb on - strand, within AZOBR_RS04080 at 859.298 kb on - strand, within AZOBR_RS04080 at 859.298 kb on - strand, within AZOBR_RS04080 at 859.423 kb on + strand, within AZOBR_RS04080 at 859.432 kb on + strand, within AZOBR_RS04080 at 859.433 kb on - strand, within AZOBR_RS04080 at 859.433 kb on - strand, within AZOBR_RS04080 at 859.640 kb on + strand, within AZOBR_RS04080 at 859.640 kb on + strand, within AZOBR_RS04080 at 859.685 kb on - strand at 859.685 kb on - strand at 859.687 kb on + strand at 859.687 kb on + strand at 859.687 kb on + strand at 859.688 kb on - strand at 859.688 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 856,664 + -2.6 856,664 + +0.9 856,664 + -3.6 857,314 - -1.3 857,314 - -0.8 857,396 - -0.0 857,396 - -0.2 857,396 - -0.4 857,411 - -0.4 858,855 - -0.5 859,124 - AZOBR_RS04080 0.27 +0.3 859,297 + AZOBR_RS04080 0.47 -1.8 859,297 + AZOBR_RS04080 0.47 -0.4 859,297 + AZOBR_RS04080 0.47 +0.8 859,298 - AZOBR_RS04080 0.47 +1.3 859,298 - AZOBR_RS04080 0.47 +0.3 859,298 - AZOBR_RS04080 0.47 +1.2 859,423 + AZOBR_RS04080 0.61 -0.2 859,432 + AZOBR_RS04080 0.62 -1.7 859,433 - AZOBR_RS04080 0.62 -0.0 859,433 - AZOBR_RS04080 0.62 -0.8 859,640 + AZOBR_RS04080 0.86 +1.9 859,640 + AZOBR_RS04080 0.86 -1.3 859,685 - -3.1 859,685 - +0.3 859,687 + +0.9 859,687 + +0.1 859,687 + +0.4 859,688 - +0.2 859,688 - +0.0
Or see this region's nucleotide sequence