Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS02195 and AZOBR_RS02200 overlap by 13 nucleotides AZOBR_RS02200 and AZOBR_RS02205 are separated by 144 nucleotides
AZOBR_RS02195: AZOBR_RS02195 - coproporphyrinogen III oxidase, at 450,526 to 451,692
_RS02195
AZOBR_RS02200: AZOBR_RS02200 - nucleoside-triphosphate diphosphatase, at 451,680 to 452,282
_RS02200
AZOBR_RS02205: AZOBR_RS02205 - ribonuclease PH, at 452,427 to 453,146
_RS02205
Position (kb) 451
452
453 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 450.900 kb on - strand, within AZOBR_RS02195 at 450.918 kb on - strand, within AZOBR_RS02195 at 450.918 kb on - strand, within AZOBR_RS02195 at 450.971 kb on + strand, within AZOBR_RS02195 at 451.017 kb on - strand, within AZOBR_RS02195 at 451.193 kb on + strand, within AZOBR_RS02195 at 451.194 kb on - strand, within AZOBR_RS02195 at 451.620 kb on - strand at 451.655 kb on + strand at 451.655 kb on + strand at 451.655 kb on + strand at 451.655 kb on + strand at 451.656 kb on - strand at 451.805 kb on - strand, within AZOBR_RS02200 at 452.008 kb on + strand, within AZOBR_RS02200 at 452.008 kb on + strand at 452.008 kb on + strand, within AZOBR_RS02200 at 452.008 kb on + strand, within AZOBR_RS02200 at 452.008 kb on + strand, within AZOBR_RS02200 at 452.009 kb on - strand, within AZOBR_RS02200 at 452.009 kb on - strand, within AZOBR_RS02200 at 452.428 kb on + strand at 452.428 kb on + strand at 452.428 kb on + strand at 452.429 kb on - strand at 452.429 kb on - strand at 452.647 kb on + strand, within AZOBR_RS02205 at 452.647 kb on + strand, within AZOBR_RS02205 at 452.647 kb on + strand, within AZOBR_RS02205 at 452.647 kb on + strand, within AZOBR_RS02205 at 452.647 kb on + strand, within AZOBR_RS02205 at 452.647 kb on + strand, within AZOBR_RS02205 at 453.186 kb on + strand at 453.186 kb on + strand at 453.187 kb on - strand at 453.187 kb on - strand at 453.255 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 450,900 - AZOBR_RS02195 0.32 +0.4 450,918 - AZOBR_RS02195 0.34 -0.2 450,918 - AZOBR_RS02195 0.34 -1.7 450,971 + AZOBR_RS02195 0.38 -0.6 451,017 - AZOBR_RS02195 0.42 -2.1 451,193 + AZOBR_RS02195 0.57 -0.5 451,194 - AZOBR_RS02195 0.57 -0.6 451,620 - +0.1 451,655 + -0.2 451,655 + -0.9 451,655 + -0.2 451,655 + +1.4 451,656 - -0.1 451,805 - AZOBR_RS02200 0.21 +1.0 452,008 + AZOBR_RS02200 0.54 +0.4 452,008 + +0.9 452,008 + AZOBR_RS02200 0.54 +0.5 452,008 + AZOBR_RS02200 0.54 +0.3 452,008 + AZOBR_RS02200 0.54 +0.7 452,009 - AZOBR_RS02200 0.55 -0.3 452,009 - AZOBR_RS02200 0.55 +2.8 452,428 + +0.2 452,428 + +0.7 452,428 + -2.1 452,429 - -2.1 452,429 - +0.6 452,647 + AZOBR_RS02205 0.31 -1.5 452,647 + AZOBR_RS02205 0.31 -0.8 452,647 + AZOBR_RS02205 0.31 -0.5 452,647 + AZOBR_RS02205 0.31 -0.1 452,647 + AZOBR_RS02205 0.31 -1.9 452,647 + AZOBR_RS02205 0.31 +0.5 453,186 + -0.2 453,186 + +1.7 453,187 - -1.3 453,187 - -0.6 453,255 - +0.3
Or see this region's nucleotide sequence