Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS02175 and AZOBR_RS02180 are separated by 92 nucleotides AZOBR_RS02180 and AZOBR_RS02185 overlap by 17 nucleotides AZOBR_RS02185 and AZOBR_RS02190 are separated by 32 nucleotides AZOBR_RS02190 and AZOBR_RS02195 are separated by 189 nucleotides
AZOBR_RS02175: AZOBR_RS02175 - hypothetical protein, at 447,449 to 447,787
_RS02175
AZOBR_RS02180: AZOBR_RS02180 - hypothetical protein, at 447,880 to 448,122
_RS02180
AZOBR_RS02185: AZOBR_RS02185 - dihydrofolate reductase, at 448,106 to 448,612
_RS02185
AZOBR_RS02190: AZOBR_RS02190 - methyl-accepting chemotaxis protein, at 448,645 to 450,336
_RS02190
AZOBR_RS02195: AZOBR_RS02195 - coproporphyrinogen III oxidase, at 450,526 to 451,692
_RS02195
Position (kb) 448
449
450
451 Strain fitness (log2 ratio) -2
-1
0
1 at 447.902 kb on - strand at 447.973 kb on + strand, within AZOBR_RS02180 at 448.021 kb on + strand, within AZOBR_RS02180 at 448.022 kb on - strand, within AZOBR_RS02180 at 448.146 kb on + strand at 448.146 kb on + strand at 448.147 kb on - strand at 448.153 kb on - strand at 448.646 kb on + strand at 448.647 kb on - strand at 448.729 kb on + strand at 448.730 kb on - strand at 449.852 kb on + strand, within AZOBR_RS02190 at 449.852 kb on + strand, within AZOBR_RS02190 at 449.852 kb on + strand, within AZOBR_RS02190 at 449.852 kb on + strand, within AZOBR_RS02190 at 449.853 kb on - strand, within AZOBR_RS02190 at 449.853 kb on - strand, within AZOBR_RS02190 at 449.853 kb on - strand, within AZOBR_RS02190 at 449.853 kb on - strand, within AZOBR_RS02190 at 449.903 kb on + strand, within AZOBR_RS02190 at 449.904 kb on - strand, within AZOBR_RS02190 at 450.059 kb on + strand, within AZOBR_RS02190 at 450.059 kb on + strand, within AZOBR_RS02190 at 450.059 kb on + strand, within AZOBR_RS02190 at 450.060 kb on - strand, within AZOBR_RS02190 at 450.060 kb on - strand, within AZOBR_RS02190 at 450.242 kb on + strand at 450.243 kb on - strand at 450.446 kb on + strand at 450.458 kb on + strand at 450.460 kb on + strand at 450.461 kb on - strand at 450.900 kb on - strand, within AZOBR_RS02195 at 450.918 kb on - strand, within AZOBR_RS02195 at 450.918 kb on - strand, within AZOBR_RS02195 at 450.971 kb on + strand, within AZOBR_RS02195 at 451.017 kb on - strand, within AZOBR_RS02195 at 451.193 kb on + strand, within AZOBR_RS02195 at 451.194 kb on - strand, within AZOBR_RS02195
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 447,902 - +0.2 447,973 + AZOBR_RS02180 0.38 -1.2 448,021 + AZOBR_RS02180 0.58 +0.5 448,022 - AZOBR_RS02180 0.58 -0.3 448,146 + +0.3 448,146 + -0.3 448,147 - +0.2 448,153 - +0.2 448,646 + -2.2 448,647 - +0.4 448,729 + -1.3 448,730 - -0.9 449,852 + AZOBR_RS02190 0.71 +0.0 449,852 + AZOBR_RS02190 0.71 +0.5 449,852 + AZOBR_RS02190 0.71 +0.0 449,852 + AZOBR_RS02190 0.71 +0.1 449,853 - AZOBR_RS02190 0.71 -2.1 449,853 - AZOBR_RS02190 0.71 +0.5 449,853 - AZOBR_RS02190 0.71 -1.7 449,853 - AZOBR_RS02190 0.71 +0.9 449,903 + AZOBR_RS02190 0.74 -2.0 449,904 - AZOBR_RS02190 0.74 +0.9 450,059 + AZOBR_RS02190 0.84 -1.4 450,059 + AZOBR_RS02190 0.84 +1.2 450,059 + AZOBR_RS02190 0.84 +0.5 450,060 - AZOBR_RS02190 0.84 +0.8 450,060 - AZOBR_RS02190 0.84 -0.1 450,242 + -1.3 450,243 - -0.2 450,446 + -0.0 450,458 + +0.2 450,460 + -2.0 450,461 - +0.5 450,900 - AZOBR_RS02195 0.32 +0.4 450,918 - AZOBR_RS02195 0.34 -0.2 450,918 - AZOBR_RS02195 0.34 -1.7 450,971 + AZOBR_RS02195 0.38 -0.6 451,017 - AZOBR_RS02195 0.42 -2.1 451,193 + AZOBR_RS02195 0.57 -0.5 451,194 - AZOBR_RS02195 0.57 -0.6
Or see this region's nucleotide sequence