Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS01315 and AZOBR_RS01320 are separated by 58 nucleotides AZOBR_RS01320 and AZOBR_RS01325 are separated by 22 nucleotides
AZOBR_RS01315: AZOBR_RS01315 - GCN5 family N-acetyltransferase, at 273,136 to 275,820
_RS01315
AZOBR_RS01320: AZOBR_RS01320 - hypothetical protein, at 275,879 to 276,451
_RS01320
AZOBR_RS01325: AZOBR_RS01325 - 2-hydroxyacid dehydrogenase, at 276,474 to 277,463
_RS01325
Position (kb) 275
276
277 Strain fitness (log2 ratio) -2
-1
0
1 at 274.894 kb on + strand, within AZOBR_RS01315 at 274.894 kb on + strand, within AZOBR_RS01315 at 274.895 kb on - strand, within AZOBR_RS01315 at 274.987 kb on + strand at 275.471 kb on - strand, within AZOBR_RS01315 at 275.533 kb on + strand, within AZOBR_RS01315 at 275.533 kb on + strand, within AZOBR_RS01315 at 275.533 kb on + strand, within AZOBR_RS01315 at 275.534 kb on - strand, within AZOBR_RS01315 at 275.534 kb on - strand at 275.582 kb on - strand at 275.626 kb on + strand at 275.683 kb on + strand at 275.874 kb on + strand at 275.874 kb on + strand at 275.874 kb on + strand at 275.875 kb on - strand at 275.875 kb on - strand at 275.875 kb on - strand at 276.116 kb on + strand, within AZOBR_RS01320 at 276.116 kb on + strand, within AZOBR_RS01320 at 276.116 kb on + strand, within AZOBR_RS01320 at 276.116 kb on + strand, within AZOBR_RS01320 at 276.434 kb on + strand at 276.434 kb on + strand at 276.435 kb on - strand at 276.450 kb on - strand at 276.475 kb on + strand at 276.476 kb on - strand at 276.476 kb on - strand at 276.799 kb on + strand, within AZOBR_RS01325 at 276.799 kb on + strand, within AZOBR_RS01325 at 276.874 kb on + strand, within AZOBR_RS01325 at 276.874 kb on + strand, within AZOBR_RS01325 at 276.928 kb on + strand, within AZOBR_RS01325
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 274,894 + AZOBR_RS01315 0.65 -0.4 274,894 + AZOBR_RS01315 0.65 +0.1 274,895 - AZOBR_RS01315 0.66 +0.6 274,987 + +0.5 275,471 - AZOBR_RS01315 0.87 +0.9 275,533 + AZOBR_RS01315 0.89 -1.4 275,533 + AZOBR_RS01315 0.89 -0.9 275,533 + AZOBR_RS01315 0.89 +0.8 275,534 - AZOBR_RS01315 0.89 -0.0 275,534 - +0.5 275,582 - -1.0 275,626 + -0.7 275,683 + -2.7 275,874 + +0.3 275,874 + -0.5 275,874 + -1.7 275,875 - -0.5 275,875 - -1.8 275,875 - +0.5 276,116 + AZOBR_RS01320 0.41 -0.5 276,116 + AZOBR_RS01320 0.41 -0.2 276,116 + AZOBR_RS01320 0.41 -2.5 276,116 + AZOBR_RS01320 0.41 -2.4 276,434 + +0.6 276,434 + +1.1 276,435 - -0.9 276,450 - -0.4 276,475 + -0.3 276,476 - -0.2 276,476 - +0.1 276,799 + AZOBR_RS01325 0.33 -2.2 276,799 + AZOBR_RS01325 0.33 -2.4 276,874 + AZOBR_RS01325 0.40 -0.2 276,874 + AZOBR_RS01325 0.40 +0.1 276,928 + AZOBR_RS01325 0.46 +0.2
Or see this region's nucleotide sequence