Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_29450 and AO356_29455 are separated by 176 nucleotides AO356_29455 and AO356_29460 are separated by 39 nucleotides AO356_29460 and AO356_29465 are separated by 112 nucleotides
AO356_29450: AO356_29450 - ornithine cyclodeaminase, at 6,852,460 to 6,853,515
_29450
AO356_29455: AO356_29455 - amino acid transporter, at 6,853,692 to 6,855,146
_29455
AO356_29460: AO356_29460 - hypothetical protein, at 6,855,186 to 6,855,722
_29460
AO356_29465: AO356_29465 - thiamine biosynthesis protein ThiJ, at 6,855,835 to 6,856,461
_29465
Position (kb)
6853
6854
6855
6856 Strain fitness (log2 ratio)
-2
-1
0
1 at 6853.078 kb on + strand, within AO356_29450 at 6853.078 kb on + strand, within AO356_29450 at 6853.122 kb on + strand, within AO356_29450 at 6853.122 kb on + strand, within AO356_29450 at 6853.122 kb on + strand, within AO356_29450 at 6853.122 kb on + strand, within AO356_29450 at 6853.122 kb on + strand, within AO356_29450 at 6853.122 kb on + strand, within AO356_29450 at 6853.123 kb on - strand, within AO356_29450 at 6853.123 kb on - strand, within AO356_29450 at 6853.123 kb on - strand, within AO356_29450 at 6853.133 kb on + strand, within AO356_29450 at 6853.133 kb on + strand, within AO356_29450 at 6853.134 kb on - strand, within AO356_29450 at 6853.376 kb on - strand, within AO356_29450 at 6853.376 kb on - strand, within AO356_29450 at 6853.376 kb on - strand, within AO356_29450 at 6853.446 kb on - strand at 6853.457 kb on - strand at 6853.522 kb on + strand at 6853.522 kb on + strand at 6853.523 kb on - strand at 6853.523 kb on - strand at 6853.634 kb on - strand at 6853.766 kb on - strand at 6853.766 kb on - strand at 6853.855 kb on - strand, within AO356_29455 at 6853.855 kb on - strand, within AO356_29455 at 6853.957 kb on - strand, within AO356_29455 at 6853.996 kb on - strand, within AO356_29455 at 6854.058 kb on + strand, within AO356_29455 at 6854.099 kb on + strand, within AO356_29455 at 6854.156 kb on - strand, within AO356_29455 at 6854.156 kb on - strand, within AO356_29455 at 6854.455 kb on - strand, within AO356_29455 at 6854.455 kb on - strand, within AO356_29455 at 6854.699 kb on + strand, within AO356_29455 at 6854.700 kb on - strand, within AO356_29455 at 6854.737 kb on + strand, within AO356_29455 at 6854.738 kb on - strand, within AO356_29455 at 6854.804 kb on + strand, within AO356_29455 at 6854.810 kb on - strand, within AO356_29455 at 6854.856 kb on + strand, within AO356_29455 at 6854.856 kb on + strand, within AO356_29455 at 6854.856 kb on + strand, within AO356_29455 at 6854.857 kb on - strand, within AO356_29455 at 6854.857 kb on - strand, within AO356_29455 at 6854.996 kb on + strand, within AO356_29455 at 6854.996 kb on + strand, within AO356_29455 at 6854.997 kb on - strand, within AO356_29455 at 6855.098 kb on + strand at 6855.145 kb on - strand at 6855.286 kb on + strand, within AO356_29460 at 6855.286 kb on + strand, within AO356_29460 at 6855.286 kb on + strand, within AO356_29460 at 6855.442 kb on + strand, within AO356_29460 at 6855.443 kb on - strand, within AO356_29460 at 6855.655 kb on + strand, within AO356_29460 at 6855.655 kb on + strand, within AO356_29460 at 6855.655 kb on + strand, within AO356_29460 at 6855.742 kb on + strand at 6855.797 kb on + strand at 6855.798 kb on - strand at 6855.798 kb on - strand at 6855.905 kb on + strand, within AO356_29465 at 6855.905 kb on + strand, within AO356_29465 at 6855.905 kb on + strand, within AO356_29465 at 6855.906 kb on - strand, within AO356_29465 at 6855.906 kb on - strand, within AO356_29465 at 6856.046 kb on - strand, within AO356_29465 at 6856.111 kb on - strand, within AO356_29465 at 6856.111 kb on - strand, within AO356_29465 at 6856.111 kb on - strand, within AO356_29465
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 6,853,078 + AO356_29450 0.59 -0.2 6,853,078 + AO356_29450 0.59 -0.7 6,853,122 + AO356_29450 0.63 -0.1 6,853,122 + AO356_29450 0.63 +0.5 6,853,122 + AO356_29450 0.63 +1.7 6,853,122 + AO356_29450 0.63 -0.2 6,853,122 + AO356_29450 0.63 -0.4 6,853,122 + AO356_29450 0.63 +0.4 6,853,123 - AO356_29450 0.63 +0.9 6,853,123 - AO356_29450 0.63 +1.0 6,853,123 - AO356_29450 0.63 -0.8 6,853,133 + AO356_29450 0.64 -0.2 6,853,133 + AO356_29450 0.64 +0.5 6,853,134 - AO356_29450 0.64 -0.7 6,853,376 - AO356_29450 0.87 -0.1 6,853,376 - AO356_29450 0.87 -0.6 6,853,376 - AO356_29450 0.87 +0.4 6,853,446 - -0.2 6,853,457 - -0.4 6,853,522 + -0.9 6,853,522 + -0.0 6,853,523 - -0.2 6,853,523 - -0.2 6,853,634 - -1.6 6,853,766 - -0.7 6,853,766 - +1.3 6,853,855 - AO356_29455 0.11 +0.3 6,853,855 - AO356_29455 0.11 +0.8 6,853,957 - AO356_29455 0.18 +0.3 6,853,996 - AO356_29455 0.21 +1.0 6,854,058 + AO356_29455 0.25 -0.6 6,854,099 + AO356_29455 0.28 +0.9 6,854,156 - AO356_29455 0.32 +1.1 6,854,156 - AO356_29455 0.32 -0.8 6,854,455 - AO356_29455 0.52 -0.5 6,854,455 - AO356_29455 0.52 -0.9 6,854,699 + AO356_29455 0.69 -0.3 6,854,700 - AO356_29455 0.69 -0.3 6,854,737 + AO356_29455 0.72 +0.1 6,854,738 - AO356_29455 0.72 -0.2 6,854,804 + AO356_29455 0.76 +0.9 6,854,810 - AO356_29455 0.77 +0.9 6,854,856 + AO356_29455 0.80 +0.1 6,854,856 + AO356_29455 0.80 +0.8 6,854,856 + AO356_29455 0.80 +0.1 6,854,857 - AO356_29455 0.80 -0.5 6,854,857 - AO356_29455 0.80 +0.0 6,854,996 + AO356_29455 0.90 -0.1 6,854,996 + AO356_29455 0.90 -0.7 6,854,997 - AO356_29455 0.90 +0.8 6,855,098 + -1.8 6,855,145 - +0.5 6,855,286 + AO356_29460 0.19 -0.1 6,855,286 + AO356_29460 0.19 -0.0 6,855,286 + AO356_29460 0.19 +1.2 6,855,442 + AO356_29460 0.48 -1.1 6,855,443 - AO356_29460 0.48 -0.9 6,855,655 + AO356_29460 0.87 -1.4 6,855,655 + AO356_29460 0.87 -1.1 6,855,655 + AO356_29460 0.87 -0.9 6,855,742 + +1.0 6,855,797 + +0.7 6,855,798 - -1.2 6,855,798 - -0.2 6,855,905 + AO356_29465 0.11 -0.2 6,855,905 + AO356_29465 0.11 -0.0 6,855,905 + AO356_29465 0.11 +0.4 6,855,906 - AO356_29465 0.11 +0.2 6,855,906 - AO356_29465 0.11 -0.8 6,856,046 - AO356_29465 0.34 -1.6 6,856,111 - AO356_29465 0.44 -1.0 6,856,111 - AO356_29465 0.44 -0.3 6,856,111 - AO356_29465 0.44 -0.2
Or see this region's nucleotide sequence