Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_23245 and AO356_23250 are separated by 138 nucleotides AO356_23250 and AO356_23255 are separated by 201 nucleotides AO356_23255 and AO356_23260 overlap by 4 nucleotides AO356_23260 and AO356_23265 overlap by 4 nucleotides AO356_23265 and AO356_23270 overlap by 4 nucleotides
AO356_23245: AO356_23245 - ABC transporter substrate-binding protein, at 5,202,262 to 5,203,272
_23245
AO356_23250: AO356_23250 - hypothetical protein, at 5,203,411 to 5,203,848
_23250
AO356_23255: AO356_23255 - cation:proton antiporter, at 5,204,050 to 5,204,412
_23255
AO356_23260: AO356_23260 - cation:proton antiporter, at 5,204,409 to 5,204,687
_23260
AO356_23265: AO356_23265 - cation:proton antiporter, at 5,204,684 to 5,205,172
_23265
AO356_23270: AO356_23270 - cation:proton antiporter, at 5,205,169 to 5,206,854
_23270
Position (kb) 5204
5205 Strain fitness (log2 ratio) -1
0
1
2 at 5203.270 kb on + strand at 5203.270 kb on + strand at 5203.375 kb on - strand at 5203.375 kb on - strand at 5203.375 kb on - strand at 5203.422 kb on - strand at 5203.422 kb on - strand at 5203.474 kb on + strand, within AO356_23250 at 5203.474 kb on + strand, within AO356_23250 at 5203.475 kb on - strand, within AO356_23250 at 5203.792 kb on + strand, within AO356_23250 at 5203.875 kb on + strand at 5203.875 kb on + strand at 5203.876 kb on - strand at 5203.876 kb on - strand at 5203.876 kb on - strand at 5203.876 kb on - strand at 5204.045 kb on - strand at 5204.500 kb on + strand, within AO356_23260 at 5204.501 kb on - strand, within AO356_23260 at 5204.501 kb on - strand, within AO356_23260 at 5204.501 kb on - strand, within AO356_23260 at 5204.516 kb on - strand, within AO356_23260 at 5204.557 kb on + strand, within AO356_23260 at 5204.557 kb on + strand, within AO356_23260 at 5204.558 kb on - strand, within AO356_23260 at 5204.558 kb on - strand, within AO356_23260 at 5204.558 kb on - strand, within AO356_23260 at 5204.558 kb on - strand, within AO356_23260 at 5204.558 kb on - strand, within AO356_23260 at 5204.563 kb on + strand, within AO356_23260 at 5204.563 kb on + strand, within AO356_23260 at 5204.563 kb on + strand, within AO356_23260 at 5204.563 kb on + strand, within AO356_23260 at 5204.563 kb on + strand, within AO356_23260 at 5204.564 kb on - strand, within AO356_23260 at 5204.564 kb on - strand, within AO356_23260 at 5204.715 kb on + strand at 5204.715 kb on + strand at 5204.716 kb on - strand at 5204.716 kb on - strand at 5204.716 kb on - strand at 5204.927 kb on + strand, within AO356_23265 at 5204.928 kb on - strand, within AO356_23265 at 5204.928 kb on - strand, within AO356_23265 at 5204.928 kb on - strand, within AO356_23265 at 5205.105 kb on - strand, within AO356_23265 at 5205.105 kb on - strand, within AO356_23265 at 5205.233 kb on - strand at 5205.245 kb on + strand at 5205.245 kb on + strand at 5205.245 kb on + strand at 5205.245 kb on + strand at 5205.245 kb on + strand at 5205.245 kb on + strand at 5205.246 kb on - strand at 5205.246 kb on - strand at 5205.246 kb on - strand at 5205.285 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 5,203,270 + +0.3 5,203,270 + +1.4 5,203,375 - +0.3 5,203,375 - -0.1 5,203,375 - +0.8 5,203,422 - -1.0 5,203,422 - -0.2 5,203,474 + AO356_23250 0.14 -0.9 5,203,474 + AO356_23250 0.14 +0.1 5,203,475 - AO356_23250 0.15 -1.6 5,203,792 + AO356_23250 0.87 -0.1 5,203,875 + +0.6 5,203,875 + -0.4 5,203,876 - +0.7 5,203,876 - -1.0 5,203,876 - +0.3 5,203,876 - -0.1 5,204,045 - -0.4 5,204,500 + AO356_23260 0.33 +0.0 5,204,501 - AO356_23260 0.33 -0.6 5,204,501 - AO356_23260 0.33 +0.0 5,204,501 - AO356_23260 0.33 -0.1 5,204,516 - AO356_23260 0.38 +0.2 5,204,557 + AO356_23260 0.53 +0.4 5,204,557 + AO356_23260 0.53 -1.1 5,204,558 - AO356_23260 0.53 +0.5 5,204,558 - AO356_23260 0.53 +1.6 5,204,558 - AO356_23260 0.53 +0.3 5,204,558 - AO356_23260 0.53 +0.5 5,204,558 - AO356_23260 0.53 +0.4 5,204,563 + AO356_23260 0.55 +1.5 5,204,563 + AO356_23260 0.55 +0.4 5,204,563 + AO356_23260 0.55 +1.4 5,204,563 + AO356_23260 0.55 +0.1 5,204,563 + AO356_23260 0.55 -0.1 5,204,564 - AO356_23260 0.56 +0.6 5,204,564 - AO356_23260 0.56 +0.5 5,204,715 + -0.0 5,204,715 + +0.4 5,204,716 - -0.7 5,204,716 - -0.4 5,204,716 - +2.1 5,204,927 + AO356_23265 0.50 -0.1 5,204,928 - AO356_23265 0.50 +0.3 5,204,928 - AO356_23265 0.50 +0.7 5,204,928 - AO356_23265 0.50 +1.1 5,205,105 - AO356_23265 0.86 -0.4 5,205,105 - AO356_23265 0.86 -0.2 5,205,233 - -0.4 5,205,245 + -0.4 5,205,245 + -0.9 5,205,245 + +0.5 5,205,245 + -0.3 5,205,245 + +0.1 5,205,245 + +0.9 5,205,246 - -0.1 5,205,246 - -0.3 5,205,246 - +0.2 5,205,285 + -0.2
Or see this region's nucleotide sequence