Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_21790 and AO356_21795 overlap by 4 nucleotides AO356_21795 and AO356_21800 overlap by 4 nucleotides AO356_21800 and AO356_21805 are separated by 214 nucleotides AO356_21805 and AO356_21810 overlap by 1 nucleotides
AO356_21790: AO356_21790 - sulfonate ABC transporter ATP-binding protein, at 4,877,548 to 4,878,396
_21790
AO356_21795: AO356_21795 - PBS lyase, at 4,878,393 to 4,879,355
_21795
AO356_21800: AO356_21800 - hypothetical protein, at 4,879,352 to 4,879,639
_21800
AO356_21805: AO356_21805 - LexA family transcriptional regulator, at 4,879,854 to 4,880,471
_21805
AO356_21810: AO356_21810 - CDP-6-deoxy-delta-3,4-glucoseen reductase, at 4,880,471 to 4,881,103
_21810
Position (kb)
4879
4880 Strain fitness (log2 ratio)
-1
0
1
2 at 4878.404 kb on - strand at 4878.935 kb on + strand, within AO356_21795 at 4879.120 kb on - strand, within AO356_21795 at 4879.327 kb on + strand at 4879.328 kb on - strand at 4879.725 kb on + strand at 4879.725 kb on + strand at 4879.726 kb on - strand at 4879.729 kb on + strand at 4879.729 kb on + strand at 4879.730 kb on - strand at 4879.730 kb on - strand at 4879.730 kb on - strand at 4879.736 kb on + strand at 4879.736 kb on + strand at 4879.736 kb on + strand at 4879.736 kb on + strand at 4879.737 kb on - strand at 4879.982 kb on + strand, within AO356_21805 at 4879.982 kb on + strand, within AO356_21805 at 4879.983 kb on - strand, within AO356_21805 at 4879.983 kb on - strand, within AO356_21805 at 4880.205 kb on + strand, within AO356_21805 at 4880.205 kb on + strand, within AO356_21805 at 4880.434 kb on + strand at 4880.435 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 4,878,404 - +0.1 4,878,935 + AO356_21795 0.56 -1.0 4,879,120 - AO356_21795 0.75 -1.0 4,879,327 + +0.3 4,879,328 - -0.4 4,879,725 + -0.8 4,879,725 + -0.7 4,879,726 - +0.6 4,879,729 + -0.6 4,879,729 + +0.5 4,879,730 - +0.7 4,879,730 - +0.4 4,879,730 - +0.3 4,879,736 + +0.6 4,879,736 + +0.3 4,879,736 + -0.5 4,879,736 + +0.1 4,879,737 - +0.1 4,879,982 + AO356_21805 0.21 +0.1 4,879,982 + AO356_21805 0.21 -0.1 4,879,983 - AO356_21805 0.21 +0.2 4,879,983 - AO356_21805 0.21 +0.8 4,880,205 + AO356_21805 0.57 -1.2 4,880,205 + AO356_21805 0.57 +1.9 4,880,434 + -1.3 4,880,435 - +0.1
Or see this region's nucleotide sequence