Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_14280 and AO356_14285 are separated by 8 nucleotides AO356_14285 and AO356_14290 are separated by 74 nucleotides AO356_14290 and AO356_14295 overlap by 10 nucleotides
AO356_14280: AO356_14280 - tRNA threonylcarbamoyl adenosine modification protein TsaD, at 3,248,356 to 3,249,381
_14280
AO356_14285: AO356_14285 - glycerol-3-phosphate acyltransferase, at 3,249,390 to 3,249,959
_14285
AO356_14290: AO356_14290 - dihydroneopterin aldolase, at 3,250,034 to 3,250,387
_14290
AO356_14295: AO356_14295 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 3,250,378 to 3,250,896
_14295
Position (kb)
3249
3250 Strain fitness (log2 ratio)
-1
0
1 at 3249.387 kb on + strand at 3249.388 kb on - strand at 3249.391 kb on + strand at 3249.392 kb on - strand at 3249.392 kb on - strand at 3249.426 kb on + strand at 3249.426 kb on + strand at 3249.426 kb on + strand at 3249.426 kb on + strand at 3249.427 kb on - strand at 3249.427 kb on - strand at 3249.565 kb on + strand, within AO356_14285 at 3249.565 kb on + strand, within AO356_14285 at 3249.565 kb on + strand, within AO356_14285 at 3249.566 kb on - strand, within AO356_14285 at 3249.673 kb on + strand, within AO356_14285 at 3249.673 kb on + strand, within AO356_14285 at 3249.674 kb on - strand, within AO356_14285 at 3249.674 kb on - strand, within AO356_14285 at 3249.674 kb on - strand, within AO356_14285 at 3249.931 kb on + strand at 3249.962 kb on + strand at 3249.962 kb on + strand at 3249.962 kb on + strand at 3249.962 kb on + strand at 3249.962 kb on + strand at 3249.962 kb on + strand at 3249.966 kb on + strand at 3249.966 kb on + strand at 3249.966 kb on + strand at 3249.966 kb on + strand at 3249.966 kb on + strand at 3249.966 kb on + strand at 3249.966 kb on + strand at 3250.416 kb on + strand at 3250.670 kb on - strand, within AO356_14295 at 3250.670 kb on - strand, within AO356_14295 at 3250.670 kb on - strand, within AO356_14295 at 3250.714 kb on - strand, within AO356_14295 at 3250.875 kb on + strand at 3250.875 kb on + strand at 3250.875 kb on + strand at 3250.875 kb on + strand at 3250.876 kb on - strand at 3250.876 kb on - strand at 3250.876 kb on - strand at 3250.876 kb on - strand at 3250.876 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,249,387 + +0.5 3,249,388 - -0.1 3,249,391 + +0.8 3,249,392 - +0.2 3,249,392 - +0.4 3,249,426 + +0.0 3,249,426 + +0.9 3,249,426 + -0.3 3,249,426 + -0.2 3,249,427 - +0.7 3,249,427 - +0.6 3,249,565 + AO356_14285 0.31 +1.1 3,249,565 + AO356_14285 0.31 +1.5 3,249,565 + AO356_14285 0.31 +1.2 3,249,566 - AO356_14285 0.31 -0.2 3,249,673 + AO356_14285 0.50 +1.5 3,249,673 + AO356_14285 0.50 +0.9 3,249,674 - AO356_14285 0.50 -1.4 3,249,674 - AO356_14285 0.50 +0.6 3,249,674 - AO356_14285 0.50 +1.5 3,249,931 + -0.7 3,249,962 + +0.4 3,249,962 + +1.4 3,249,962 + +0.8 3,249,962 + +0.2 3,249,962 + -0.1 3,249,962 + +0.1 3,249,966 + +0.4 3,249,966 + -0.3 3,249,966 + +1.0 3,249,966 + -1.1 3,249,966 + +0.5 3,249,966 + +0.1 3,249,966 + +0.5 3,250,416 + +1.2 3,250,670 - AO356_14295 0.56 +1.1 3,250,670 - AO356_14295 0.56 +1.4 3,250,670 - AO356_14295 0.56 -1.1 3,250,714 - AO356_14295 0.65 -0.0 3,250,875 + +0.3 3,250,875 + +0.0 3,250,875 + +0.3 3,250,875 + +1.3 3,250,876 - +0.4 3,250,876 - +0.4 3,250,876 - +1.2 3,250,876 - -1.3 3,250,876 - -1.2
Or see this region's nucleotide sequence