Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_13375 and AO356_13380 are separated by 9 nucleotides AO356_13380 and AO356_13385 are separated by 145 nucleotides AO356_13385 and AO356_13390 are separated by 18 nucleotides
AO356_13375: AO356_13375 - thiazole synthase, at 3,037,281 to 3,038,075
_13375
AO356_13380: AO356_13380 - tRNA (guanine-N7)-methyltransferase, at 3,038,085 to 3,038,810
_13380
AO356_13385: AO356_13385 - hypothetical protein, at 3,038,956 to 3,039,279
_13385
AO356_13390: AO356_13390 - coproporphyrinogen III oxidase, at 3,039,298 to 3,040,497
_13390
Position (kb)
3038
3039
3040 Strain fitness (log2 ratio)
-1
0
1
2 at 3038.004 kb on + strand at 3038.004 kb on + strand at 3038.005 kb on - strand at 3038.073 kb on + strand at 3038.073 kb on + strand at 3038.073 kb on + strand at 3038.073 kb on + strand at 3038.073 kb on + strand at 3038.073 kb on + strand at 3038.074 kb on - strand at 3038.074 kb on - strand at 3038.074 kb on - strand at 3038.074 kb on - strand at 3038.074 kb on - strand at 3038.074 kb on - strand at 3038.074 kb on - strand at 3038.074 kb on - strand at 3038.443 kb on - strand, within AO356_13380 at 3038.556 kb on - strand, within AO356_13380 at 3038.640 kb on + strand, within AO356_13380 at 3038.649 kb on + strand, within AO356_13380 at 3038.649 kb on + strand, within AO356_13380 at 3038.649 kb on + strand, within AO356_13380 at 3038.649 kb on + strand, within AO356_13380 at 3038.649 kb on + strand, within AO356_13380 at 3038.649 kb on + strand, within AO356_13380 at 3038.649 kb on + strand, within AO356_13380 at 3038.650 kb on - strand, within AO356_13380 at 3038.650 kb on - strand, within AO356_13380 at 3038.650 kb on - strand, within AO356_13380 at 3038.650 kb on - strand, within AO356_13380 at 3038.650 kb on - strand, within AO356_13380 at 3038.808 kb on + strand at 3038.808 kb on + strand at 3038.808 kb on + strand at 3038.809 kb on - strand at 3038.809 kb on - strand at 3038.809 kb on - strand at 3038.809 kb on - strand at 3038.900 kb on + strand at 3038.900 kb on + strand at 3038.900 kb on + strand at 3038.900 kb on + strand at 3038.901 kb on - strand at 3038.901 kb on - strand at 3038.901 kb on - strand at 3038.901 kb on - strand at 3038.901 kb on - strand at 3038.901 kb on - strand at 3038.901 kb on - strand at 3038.901 kb on - strand at 3038.903 kb on - strand at 3038.903 kb on - strand at 3038.944 kb on + strand at 3038.944 kb on + strand at 3038.944 kb on + strand at 3038.944 kb on + strand at 3038.944 kb on + strand at 3038.944 kb on + strand at 3038.945 kb on - strand at 3038.945 kb on - strand at 3039.020 kb on + strand, within AO356_13385 at 3039.020 kb on + strand, within AO356_13385 at 3039.020 kb on + strand, within AO356_13385 at 3039.020 kb on + strand, within AO356_13385 at 3039.020 kb on + strand, within AO356_13385 at 3039.020 kb on + strand, within AO356_13385 at 3039.020 kb on + strand, within AO356_13385 at 3039.021 kb on - strand, within AO356_13385 at 3039.021 kb on - strand, within AO356_13385 at 3039.021 kb on - strand, within AO356_13385 at 3039.021 kb on - strand, within AO356_13385 at 3039.021 kb on - strand, within AO356_13385 at 3039.065 kb on + strand, within AO356_13385 at 3039.065 kb on + strand, within AO356_13385 at 3039.065 kb on + strand, within AO356_13385 at 3039.065 kb on + strand, within AO356_13385 at 3039.065 kb on + strand, within AO356_13385 at 3039.065 kb on + strand, within AO356_13385 at 3039.066 kb on - strand, within AO356_13385 at 3039.066 kb on - strand, within AO356_13385 at 3039.066 kb on - strand, within AO356_13385 at 3039.066 kb on - strand, within AO356_13385 at 3039.066 kb on - strand, within AO356_13385 at 3039.066 kb on - strand, within AO356_13385 at 3039.066 kb on - strand, within AO356_13385 at 3039.066 kb on - strand, within AO356_13385 at 3039.199 kb on + strand, within AO356_13385 at 3039.199 kb on + strand, within AO356_13385 at 3039.199 kb on + strand, within AO356_13385 at 3039.200 kb on - strand, within AO356_13385 at 3039.200 kb on - strand, within AO356_13385 at 3039.200 kb on - strand, within AO356_13385 at 3039.200 kb on - strand, within AO356_13385 at 3039.200 kb on - strand, within AO356_13385 at 3039.200 kb on - strand, within AO356_13385 at 3039.200 kb on - strand, within AO356_13385 at 3039.200 kb on - strand, within AO356_13385 at 3039.200 kb on - strand, within AO356_13385 at 3039.269 kb on - strand at 3039.569 kb on + strand, within AO356_13390 at 3039.569 kb on + strand, within AO356_13390 at 3039.569 kb on + strand, within AO356_13390 at 3039.569 kb on + strand, within AO356_13390 at 3039.570 kb on - strand, within AO356_13390 at 3039.570 kb on - strand, within AO356_13390 at 3039.677 kb on + strand, within AO356_13390 at 3039.677 kb on + strand, within AO356_13390 at 3039.678 kb on - strand, within AO356_13390 at 3039.678 kb on - strand, within AO356_13390 at 3039.678 kb on - strand, within AO356_13390 at 3039.687 kb on + strand, within AO356_13390 at 3039.687 kb on + strand, within AO356_13390 at 3039.687 kb on + strand, within AO356_13390 at 3039.688 kb on - strand, within AO356_13390 at 3039.731 kb on + strand, within AO356_13390 at 3039.731 kb on + strand, within AO356_13390 at 3040.017 kb on + strand, within AO356_13390 at 3040.017 kb on + strand, within AO356_13390
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,038,004 + +0.8 3,038,004 + +0.5 3,038,005 - +1.6 3,038,073 + -0.3 3,038,073 + -0.5 3,038,073 + +1.2 3,038,073 + +2.1 3,038,073 + +0.5 3,038,073 + +1.3 3,038,074 - +1.5 3,038,074 - +1.4 3,038,074 - +1.6 3,038,074 - +1.2 3,038,074 - -0.5 3,038,074 - +0.7 3,038,074 - +0.8 3,038,074 - +0.6 3,038,443 - AO356_13380 0.49 +0.8 3,038,556 - AO356_13380 0.65 +1.1 3,038,640 + AO356_13380 0.76 +0.4 3,038,649 + AO356_13380 0.78 +1.1 3,038,649 + AO356_13380 0.78 +0.8 3,038,649 + AO356_13380 0.78 +0.6 3,038,649 + AO356_13380 0.78 +1.1 3,038,649 + AO356_13380 0.78 +1.7 3,038,649 + AO356_13380 0.78 +2.5 3,038,649 + AO356_13380 0.78 -0.0 3,038,650 - AO356_13380 0.78 +1.6 3,038,650 - AO356_13380 0.78 +0.1 3,038,650 - AO356_13380 0.78 +0.3 3,038,650 - AO356_13380 0.78 +1.0 3,038,650 - AO356_13380 0.78 +1.3 3,038,808 + -0.2 3,038,808 + -0.1 3,038,808 + +0.1 3,038,809 - +0.0 3,038,809 - +0.8 3,038,809 - -0.6 3,038,809 - -1.1 3,038,900 + -0.9 3,038,900 + +0.8 3,038,900 + +0.6 3,038,900 + -0.3 3,038,901 - -0.5 3,038,901 - +0.4 3,038,901 - +0.5 3,038,901 - +0.4 3,038,901 - -0.2 3,038,901 - +0.2 3,038,901 - +1.2 3,038,901 - +0.7 3,038,903 - +1.0 3,038,903 - -1.6 3,038,944 + +1.3 3,038,944 + -0.1 3,038,944 + -0.2 3,038,944 + +0.6 3,038,944 + +0.9 3,038,944 + +0.7 3,038,945 - +0.1 3,038,945 - +0.4 3,039,020 + AO356_13385 0.20 +1.4 3,039,020 + AO356_13385 0.20 +0.5 3,039,020 + AO356_13385 0.20 -0.3 3,039,020 + AO356_13385 0.20 +0.3 3,039,020 + AO356_13385 0.20 -0.8 3,039,020 + AO356_13385 0.20 -0.1 3,039,020 + AO356_13385 0.20 +0.4 3,039,021 - AO356_13385 0.20 +0.8 3,039,021 - AO356_13385 0.20 -0.7 3,039,021 - AO356_13385 0.20 +0.7 3,039,021 - AO356_13385 0.20 -0.1 3,039,021 - AO356_13385 0.20 +0.4 3,039,065 + AO356_13385 0.34 -0.2 3,039,065 + AO356_13385 0.34 +0.2 3,039,065 + AO356_13385 0.34 +0.5 3,039,065 + AO356_13385 0.34 -1.3 3,039,065 + AO356_13385 0.34 +0.7 3,039,065 + AO356_13385 0.34 +0.2 3,039,066 - AO356_13385 0.34 -0.4 3,039,066 - AO356_13385 0.34 +0.5 3,039,066 - AO356_13385 0.34 +0.6 3,039,066 - AO356_13385 0.34 -0.0 3,039,066 - AO356_13385 0.34 +0.3 3,039,066 - AO356_13385 0.34 +0.1 3,039,066 - AO356_13385 0.34 +0.3 3,039,066 - AO356_13385 0.34 +0.2 3,039,199 + AO356_13385 0.75 +1.1 3,039,199 + AO356_13385 0.75 +1.1 3,039,199 + AO356_13385 0.75 +0.6 3,039,200 - AO356_13385 0.75 -1.4 3,039,200 - AO356_13385 0.75 -1.1 3,039,200 - AO356_13385 0.75 -0.9 3,039,200 - AO356_13385 0.75 +1.0 3,039,200 - AO356_13385 0.75 -0.7 3,039,200 - AO356_13385 0.75 -0.3 3,039,200 - AO356_13385 0.75 +0.5 3,039,200 - AO356_13385 0.75 +0.7 3,039,200 - AO356_13385 0.75 -0.4 3,039,269 - -0.5 3,039,569 + AO356_13390 0.23 +1.4 3,039,569 + AO356_13390 0.23 +0.4 3,039,569 + AO356_13390 0.23 -0.3 3,039,569 + AO356_13390 0.23 +0.2 3,039,570 - AO356_13390 0.23 +0.9 3,039,570 - AO356_13390 0.23 +0.7 3,039,677 + AO356_13390 0.32 +1.1 3,039,677 + AO356_13390 0.32 -0.7 3,039,678 - AO356_13390 0.32 +0.9 3,039,678 - AO356_13390 0.32 +0.6 3,039,678 - AO356_13390 0.32 -0.3 3,039,687 + AO356_13390 0.32 +0.4 3,039,687 + AO356_13390 0.32 +0.7 3,039,687 + AO356_13390 0.32 +1.1 3,039,688 - AO356_13390 0.33 -0.3 3,039,731 + AO356_13390 0.36 +1.0 3,039,731 + AO356_13390 0.36 -0.5 3,040,017 + AO356_13390 0.60 +0.2 3,040,017 + AO356_13390 0.60 +1.5
Or see this region's nucleotide sequence