Strain Fitness in Pseudomonas fluorescens FW300-N2C3 around AO356_04215

Experiment: Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO356_04210 and AO356_04215 are separated by 193 nucleotidesAO356_04215 and AO356_04220 are separated by 112 nucleotides AO356_04210: AO356_04210 - RNA helicase, at 955,984 to 957,330 _04210 AO356_04215: AO356_04215 - mechanosensitive ion channel protein MscS, at 957,524 to 958,822 _04215 AO356_04220: AO356_04220 - hypothetical protein, at 958,935 to 959,801 _04220 Position (kb) 957 958 959Strain fitness (log2 ratio) -1 0 1 2at 957.369 kb on - strandat 957.407 kb on + strandat 957.408 kb on - strandat 957.436 kb on - strandat 957.474 kb on + strandat 957.474 kb on + strandat 957.475 kb on - strandat 957.483 kb on + strandat 957.483 kb on + strandat 957.484 kb on - strandat 957.505 kb on + strandat 957.505 kb on + strandat 957.505 kb on + strandat 957.506 kb on - strandat 957.506 kb on - strandat 957.506 kb on - strandat 957.506 kb on - strandat 957.506 kb on - strandat 957.657 kb on - strand, within AO356_04215at 957.950 kb on + strand, within AO356_04215at 957.950 kb on + strand, within AO356_04215at 957.951 kb on - strand, within AO356_04215at 957.951 kb on - strand, within AO356_04215at 957.951 kb on - strand, within AO356_04215at 957.951 kb on - strand, within AO356_04215at 957.977 kb on + strand, within AO356_04215at 957.977 kb on + strand, within AO356_04215at 957.977 kb on + strand, within AO356_04215at 957.977 kb on + strand, within AO356_04215at 957.977 kb on + strand, within AO356_04215at 957.977 kb on + strand, within AO356_04215at 957.977 kb on + strand, within AO356_04215at 957.977 kb on + strand, within AO356_04215at 957.977 kb on + strand, within AO356_04215at 957.977 kb on + strand, within AO356_04215at 957.978 kb on - strand, within AO356_04215at 957.978 kb on - strand, within AO356_04215at 957.978 kb on - strand, within AO356_04215at 957.978 kb on - strand, within AO356_04215at 957.978 kb on - strand, within AO356_04215at 957.978 kb on - strand, within AO356_04215at 957.978 kb on - strand, within AO356_04215at 957.978 kb on - strand, within AO356_04215at 957.978 kb on - strand, within AO356_04215at 958.079 kb on + strand, within AO356_04215at 958.079 kb on + strand, within AO356_04215at 958.080 kb on - strand, within AO356_04215at 958.413 kb on - strand, within AO356_04215at 958.413 kb on - strand, within AO356_04215at 958.413 kb on - strand, within AO356_04215at 958.536 kb on - strand, within AO356_04215at 958.626 kb on - strand, within AO356_04215at 958.659 kb on - strand, within AO356_04215at 958.659 kb on - strand, within AO356_04215at 958.678 kb on + strand, within AO356_04215at 958.689 kb on - strand, within AO356_04215at 958.689 kb on - strand, within AO356_04215at 958.728 kb on - strandat 958.728 kb on - strandat 958.871 kb on + strandat 958.882 kb on + strandat 958.883 kb on - strandat 958.883 kb on - strandat 958.883 kb on - strandat 958.992 kb on + strandat 959.010 kb on + strandat 959.010 kb on + strandat 959.140 kb on + strand, within AO356_04220at 959.140 kb on + strand, within AO356_04220at 959.141 kb on - strand, within AO356_04220at 959.141 kb on - strand, within AO356_04220at 959.270 kb on - strand, within AO356_04220at 959.270 kb on - strand, within AO356_04220at 959.270 kb on - strand, within AO356_04220at 959.275 kb on + strand, within AO356_04220at 959.333 kb on - strand, within AO356_04220at 959.333 kb on - strand, within AO356_04220at 959.333 kb on - strand, within AO356_04220at 959.333 kb on - strand, within AO356_04220at 959.354 kb on - strand, within AO356_04220at 959.522 kb on + strand, within AO356_04220at 959.522 kb on + strand, within AO356_04220at 959.523 kb on - strand, within AO356_04220at 959.559 kb on + strand, within AO356_04220at 959.559 kb on + strand, within AO356_04220at 959.559 kb on + strand, within AO356_04220at 959.560 kb on - strand, within AO356_04220at 959.575 kb on + strand, within AO356_04220at 959.575 kb on + strand, within AO356_04220at 959.576 kb on - strand, within AO356_04220at 959.587 kb on + strand, within AO356_04220at 959.587 kb on + strand, within AO356_04220at 959.587 kb on + strand, within AO356_04220at 959.588 kb on - strand, within AO356_04220at 959.588 kb on - strand, within AO356_04220at 959.588 kb on - strand, within AO356_04220at 959.716 kb on + strandat 959.716 kb on + strandat 959.717 kb on - strandat 959.717 kb on - strandat 959.740 kb on + strandat 959.741 kb on - strandat 959.790 kb on + strandat 959.790 kb on + strandat 959.794 kb on + strandat 959.795 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml
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957,369 - -0.1
957,407 + +0.3
957,408 - +1.3
957,436 - +2.4
957,474 + +0.7
957,474 + -0.9
957,475 - -0.7
957,483 + -0.1
957,483 + +0.1
957,484 - +0.3
957,505 + +0.5
957,505 + +1.9
957,505 + +1.1
957,506 - -0.8
957,506 - -0.0
957,506 - -0.1
957,506 - -1.1
957,506 - +0.7
957,657 - AO356_04215 0.10 -0.3
957,950 + AO356_04215 0.33 +1.1
957,950 + AO356_04215 0.33 +0.6
957,951 - AO356_04215 0.33 -0.6
957,951 - AO356_04215 0.33 +0.4
957,951 - AO356_04215 0.33 -0.9
957,951 - AO356_04215 0.33 +0.5
957,977 + AO356_04215 0.35 -0.5
957,977 + AO356_04215 0.35 +0.2
957,977 + AO356_04215 0.35 -0.4
957,977 + AO356_04215 0.35 -0.4
957,977 + AO356_04215 0.35 -0.1
957,977 + AO356_04215 0.35 -0.1
957,977 + AO356_04215 0.35 +0.0
957,977 + AO356_04215 0.35 -0.2
957,977 + AO356_04215 0.35 -0.1
957,977 + AO356_04215 0.35 +0.5
957,978 - AO356_04215 0.35 +0.4
957,978 - AO356_04215 0.35 -0.3
957,978 - AO356_04215 0.35 +0.2
957,978 - AO356_04215 0.35 +0.0
957,978 - AO356_04215 0.35 -0.2
957,978 - AO356_04215 0.35 +0.6
957,978 - AO356_04215 0.35 -0.3
957,978 - AO356_04215 0.35 -0.7
957,978 - AO356_04215 0.35 +0.1
958,079 + AO356_04215 0.43 +0.8
958,079 + AO356_04215 0.43 +0.7
958,080 - AO356_04215 0.43 -0.7
958,413 - AO356_04215 0.68 +0.4
958,413 - AO356_04215 0.68 -0.1
958,413 - AO356_04215 0.68 +0.5
958,536 - AO356_04215 0.78 -0.1
958,626 - AO356_04215 0.85 +0.1
958,659 - AO356_04215 0.87 +0.2
958,659 - AO356_04215 0.87 +0.2
958,678 + AO356_04215 0.89 -0.3
958,689 - AO356_04215 0.90 -0.1
958,689 - AO356_04215 0.90 +0.9
958,728 - -0.8
958,728 - -0.1
958,871 + +0.8
958,882 + -1.1
958,883 - +0.5
958,883 - +0.3
958,883 - +0.1
958,992 + +0.4
959,010 + +0.1
959,010 + -0.3
959,140 + AO356_04220 0.24 -1.4
959,140 + AO356_04220 0.24 -0.4
959,141 - AO356_04220 0.24 -0.2
959,141 - AO356_04220 0.24 +1.0
959,270 - AO356_04220 0.39 +1.0
959,270 - AO356_04220 0.39 +0.1
959,270 - AO356_04220 0.39 -1.1
959,275 + AO356_04220 0.39 +0.8
959,333 - AO356_04220 0.46 -0.8
959,333 - AO356_04220 0.46 +0.6
959,333 - AO356_04220 0.46 +0.2
959,333 - AO356_04220 0.46 -0.5
959,354 - AO356_04220 0.48 -1.3
959,522 + AO356_04220 0.68 +0.8
959,522 + AO356_04220 0.68 +0.6
959,523 - AO356_04220 0.68 -0.6
959,559 + AO356_04220 0.72 +0.2
959,559 + AO356_04220 0.72 +2.2
959,559 + AO356_04220 0.72 -0.9
959,560 - AO356_04220 0.72 -0.8
959,575 + AO356_04220 0.74 +1.3
959,575 + AO356_04220 0.74 +0.5
959,576 - AO356_04220 0.74 +0.3
959,587 + AO356_04220 0.75 -1.3
959,587 + AO356_04220 0.75 +0.1
959,587 + AO356_04220 0.75 +0.5
959,588 - AO356_04220 0.75 +0.5
959,588 - AO356_04220 0.75 +1.3
959,588 - AO356_04220 0.75 -0.8
959,716 + -0.2
959,716 + +0.6
959,717 - +0.2
959,717 - +0.7
959,740 + +1.3
959,741 - +0.3
959,790 + +0.5
959,790 + +0.4
959,794 + +1.1
959,795 - -0.8

Or see this region's nucleotide sequence