Experiment: KB with capsaicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3574 and Psyr_3575 are separated by 265 nucleotides Psyr_3575 and Psyr_3576 are separated by 35 nucleotides Psyr_3576 and Psyr_3577 are separated by 453 nucleotides
Psyr_3574: Psyr_3574 - Helix-turn-helix, Fis-type, at 4,256,450 to 4,258,027
_3574
Psyr_3575: Psyr_3575 - Phenylalanine 4-hydroxylase, at 4,258,293 to 4,259,090
_3575
Psyr_3576: Psyr_3576 - pterin-4-alpha-carbinolamine dehydratase, at 4,259,126 to 4,259,482
_3576
Psyr_3577: Psyr_3577 - conserved hypothetical protein, at 4,259,936 to 4,260,115
_3577
Position (kb)
4258
4259
4260 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4257.410 kb on - strand, within Psyr_3574 at 4257.410 kb on - strand, within Psyr_3574 at 4257.447 kb on + strand, within Psyr_3574 at 4257.447 kb on + strand, within Psyr_3574 at 4257.447 kb on + strand, within Psyr_3574 at 4257.447 kb on + strand, within Psyr_3574 at 4257.447 kb on + strand, within Psyr_3574 at 4257.447 kb on + strand, within Psyr_3574 at 4257.447 kb on + strand, within Psyr_3574 at 4257.447 kb on + strand, within Psyr_3574 at 4257.447 kb on + strand, within Psyr_3574 at 4257.448 kb on - strand, within Psyr_3574 at 4257.448 kb on - strand, within Psyr_3574 at 4257.448 kb on - strand, within Psyr_3574 at 4257.448 kb on - strand, within Psyr_3574 at 4257.448 kb on - strand, within Psyr_3574 at 4257.448 kb on - strand, within Psyr_3574 at 4257.448 kb on - strand, within Psyr_3574 at 4257.448 kb on - strand, within Psyr_3574 at 4257.448 kb on - strand, within Psyr_3574 at 4257.448 kb on - strand, within Psyr_3574 at 4257.719 kb on - strand, within Psyr_3574 at 4257.903 kb on + strand at 4257.903 kb on + strand at 4257.903 kb on + strand at 4257.903 kb on + strand at 4257.903 kb on + strand at 4257.903 kb on + strand at 4257.904 kb on - strand at 4257.904 kb on - strand at 4257.904 kb on - strand at 4257.904 kb on - strand at 4257.958 kb on - strand at 4258.086 kb on - strand at 4258.086 kb on - strand at 4258.086 kb on - strand at 4258.086 kb on - strand at 4258.250 kb on + strand at 4258.308 kb on + strand at 4258.308 kb on + strand at 4258.308 kb on + strand at 4258.348 kb on - strand at 4258.428 kb on + strand, within Psyr_3575 at 4258.428 kb on + strand, within Psyr_3575 at 4258.428 kb on + strand, within Psyr_3575 at 4258.629 kb on + strand, within Psyr_3575 at 4258.629 kb on + strand, within Psyr_3575 at 4258.629 kb on + strand, within Psyr_3575 at 4258.630 kb on - strand, within Psyr_3575 at 4258.660 kb on + strand, within Psyr_3575 at 4258.660 kb on + strand, within Psyr_3575 at 4258.660 kb on + strand, within Psyr_3575 at 4258.660 kb on + strand, within Psyr_3575 at 4258.661 kb on - strand, within Psyr_3575 at 4258.661 kb on - strand, within Psyr_3575 at 4258.716 kb on + strand, within Psyr_3575 at 4258.758 kb on + strand, within Psyr_3575 at 4258.759 kb on - strand, within Psyr_3575 at 4258.806 kb on - strand, within Psyr_3575 at 4258.866 kb on + strand, within Psyr_3575 at 4258.866 kb on + strand, within Psyr_3575 at 4258.867 kb on - strand, within Psyr_3575 at 4258.867 kb on - strand, within Psyr_3575 at 4258.867 kb on - strand, within Psyr_3575 at 4258.867 kb on - strand, within Psyr_3575 at 4258.867 kb on - strand, within Psyr_3575 at 4258.867 kb on - strand, within Psyr_3575 at 4258.867 kb on - strand, within Psyr_3575 at 4258.956 kb on + strand, within Psyr_3575 at 4258.956 kb on + strand, within Psyr_3575 at 4258.956 kb on + strand, within Psyr_3575 at 4258.956 kb on + strand, within Psyr_3575 at 4258.957 kb on - strand, within Psyr_3575 at 4259.066 kb on + strand at 4259.125 kb on + strand at 4259.480 kb on + strand at 4259.619 kb on + strand at 4259.676 kb on + strand at 4259.676 kb on + strand at 4259.676 kb on + strand at 4259.677 kb on - strand at 4259.677 kb on - strand at 4259.721 kb on + strand at 4259.896 kb on + strand at 4259.936 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with capsaicin 0.125 mg/ml remove 4,257,410 - Psyr_3574 0.61 -0.7 4,257,410 - Psyr_3574 0.61 +0.7 4,257,447 + Psyr_3574 0.63 +0.1 4,257,447 + Psyr_3574 0.63 +0.2 4,257,447 + Psyr_3574 0.63 +0.3 4,257,447 + Psyr_3574 0.63 -0.9 4,257,447 + Psyr_3574 0.63 -1.4 4,257,447 + Psyr_3574 0.63 -0.1 4,257,447 + Psyr_3574 0.63 -0.3 4,257,447 + Psyr_3574 0.63 -0.2 4,257,447 + Psyr_3574 0.63 -0.9 4,257,448 - Psyr_3574 0.63 -1.1 4,257,448 - Psyr_3574 0.63 +0.9 4,257,448 - Psyr_3574 0.63 +0.3 4,257,448 - Psyr_3574 0.63 -0.4 4,257,448 - Psyr_3574 0.63 +0.1 4,257,448 - Psyr_3574 0.63 -0.9 4,257,448 - Psyr_3574 0.63 +0.9 4,257,448 - Psyr_3574 0.63 +0.6 4,257,448 - Psyr_3574 0.63 -0.5 4,257,448 - Psyr_3574 0.63 +0.1 4,257,719 - Psyr_3574 0.80 +0.5 4,257,903 + -1.2 4,257,903 + -0.6 4,257,903 + -1.7 4,257,903 + +0.3 4,257,903 + -0.2 4,257,903 + -1.4 4,257,904 - +0.4 4,257,904 - -0.7 4,257,904 - -1.4 4,257,904 - +0.6 4,257,958 - -0.3 4,258,086 - -0.6 4,258,086 - +0.9 4,258,086 - +0.5 4,258,086 - +0.6 4,258,250 + -0.6 4,258,308 + -0.5 4,258,308 + +1.0 4,258,308 + +1.7 4,258,348 - +0.1 4,258,428 + Psyr_3575 0.17 +0.5 4,258,428 + Psyr_3575 0.17 +2.9 4,258,428 + Psyr_3575 0.17 +1.9 4,258,629 + Psyr_3575 0.42 -0.5 4,258,629 + Psyr_3575 0.42 -0.5 4,258,629 + Psyr_3575 0.42 +0.9 4,258,630 - Psyr_3575 0.42 -0.3 4,258,660 + Psyr_3575 0.46 +0.2 4,258,660 + Psyr_3575 0.46 +0.4 4,258,660 + Psyr_3575 0.46 +1.3 4,258,660 + Psyr_3575 0.46 +1.2 4,258,661 - Psyr_3575 0.46 +1.3 4,258,661 - Psyr_3575 0.46 +0.6 4,258,716 + Psyr_3575 0.53 -0.2 4,258,758 + Psyr_3575 0.58 -1.1 4,258,759 - Psyr_3575 0.58 -0.6 4,258,806 - Psyr_3575 0.64 -0.3 4,258,866 + Psyr_3575 0.72 +1.1 4,258,866 + Psyr_3575 0.72 -0.6 4,258,867 - Psyr_3575 0.72 -0.3 4,258,867 - Psyr_3575 0.72 +0.6 4,258,867 - Psyr_3575 0.72 -1.0 4,258,867 - Psyr_3575 0.72 +1.0 4,258,867 - Psyr_3575 0.72 -0.6 4,258,867 - Psyr_3575 0.72 +1.6 4,258,867 - Psyr_3575 0.72 -0.5 4,258,956 + Psyr_3575 0.83 +0.3 4,258,956 + Psyr_3575 0.83 -0.2 4,258,956 + Psyr_3575 0.83 +0.2 4,258,956 + Psyr_3575 0.83 +1.3 4,258,957 - Psyr_3575 0.83 +1.0 4,259,066 + -0.3 4,259,125 + -1.3 4,259,480 + +1.4 4,259,619 + +0.4 4,259,676 + +0.3 4,259,676 + +0.5 4,259,676 + +1.1 4,259,677 - +1.1 4,259,677 - +0.7 4,259,721 + -2.1 4,259,896 + +0.3 4,259,936 - -0.4
Or see this region's nucleotide sequence