Experiment: KB with capsaicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2075 and Psyr_2076 are separated by 76 nucleotides Psyr_2076 and Psyr_2077 are separated by 150 nucleotides
Psyr_2075: Psyr_2075 - 5-nucleotidase, at 2,420,539 to 2,421,459
_2075
Psyr_2076: Psyr_2076 - UspA, at 2,421,536 to 2,422,030
_2076
Psyr_2077: Psyr_2077 - regulatory protein, LysR:LysR, substrate-binding protein, at 2,422,181 to 2,423,155
_2077
Position (kb)
2421
2422
2423 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2420.559 kb on + strand at 2420.560 kb on - strand at 2420.560 kb on - strand at 2420.568 kb on - strand at 2420.620 kb on + strand at 2420.620 kb on + strand at 2420.620 kb on + strand at 2420.620 kb on + strand at 2420.620 kb on + strand at 2420.620 kb on + strand at 2420.620 kb on + strand at 2420.620 kb on + strand at 2420.620 kb on + strand at 2420.621 kb on - strand at 2420.621 kb on - strand at 2420.621 kb on - strand at 2420.621 kb on - strand at 2420.621 kb on - strand at 2420.621 kb on - strand at 2420.621 kb on - strand at 2420.621 kb on - strand at 2420.621 kb on - strand at 2420.621 kb on - strand at 2420.621 kb on - strand at 2420.621 kb on - strand at 2420.621 kb on - strand at 2420.621 kb on - strand at 2420.653 kb on + strand, within Psyr_2075 at 2420.653 kb on + strand, within Psyr_2075 at 2420.654 kb on - strand, within Psyr_2075 at 2420.876 kb on - strand, within Psyr_2075 at 2420.927 kb on + strand, within Psyr_2075 at 2420.927 kb on + strand, within Psyr_2075 at 2420.927 kb on + strand, within Psyr_2075 at 2420.927 kb on + strand, within Psyr_2075 at 2420.927 kb on + strand, within Psyr_2075 at 2420.927 kb on + strand, within Psyr_2075 at 2420.928 kb on - strand, within Psyr_2075 at 2420.928 kb on - strand, within Psyr_2075 at 2421.435 kb on - strand at 2421.535 kb on + strand at 2421.535 kb on + strand at 2421.535 kb on + strand at 2421.535 kb on + strand at 2421.535 kb on + strand at 2421.535 kb on + strand at 2421.535 kb on + strand at 2421.535 kb on + strand at 2421.536 kb on - strand at 2421.536 kb on - strand at 2421.536 kb on - strand at 2421.536 kb on - strand at 2421.536 kb on - strand at 2421.554 kb on + strand at 2421.555 kb on - strand at 2421.555 kb on - strand at 2421.555 kb on - strand at 2421.576 kb on - strand at 2421.584 kb on + strand at 2421.584 kb on + strand at 2421.584 kb on + strand at 2421.584 kb on + strand at 2421.585 kb on - strand at 2421.585 kb on - strand at 2421.585 kb on - strand at 2421.585 kb on - strand at 2421.589 kb on - strand, within Psyr_2076 at 2421.589 kb on - strand, within Psyr_2076 at 2421.635 kb on + strand, within Psyr_2076 at 2421.635 kb on + strand, within Psyr_2076 at 2421.635 kb on + strand, within Psyr_2076 at 2421.635 kb on + strand, within Psyr_2076 at 2421.636 kb on - strand, within Psyr_2076 at 2421.636 kb on - strand, within Psyr_2076 at 2421.692 kb on + strand, within Psyr_2076 at 2421.693 kb on - strand, within Psyr_2076 at 2421.693 kb on - strand, within Psyr_2076 at 2421.693 kb on - strand, within Psyr_2076 at 2421.756 kb on + strand, within Psyr_2076 at 2421.757 kb on - strand, within Psyr_2076 at 2421.757 kb on - strand, within Psyr_2076 at 2421.757 kb on - strand, within Psyr_2076 at 2421.826 kb on + strand, within Psyr_2076 at 2421.993 kb on - strand at 2421.993 kb on - strand at 2421.993 kb on - strand at 2422.133 kb on + strand at 2422.135 kb on + strand at 2422.160 kb on + strand at 2422.161 kb on - strand at 2422.176 kb on + strand at 2422.176 kb on + strand at 2422.177 kb on - strand at 2422.177 kb on - strand at 2422.180 kb on + strand at 2422.180 kb on + strand at 2422.180 kb on + strand at 2422.180 kb on + strand at 2422.180 kb on + strand at 2422.180 kb on + strand at 2422.180 kb on + strand at 2422.180 kb on + strand at 2422.180 kb on + strand at 2422.180 kb on + strand at 2422.180 kb on + strand at 2422.180 kb on + strand at 2422.180 kb on + strand at 2422.180 kb on + strand at 2422.180 kb on + strand at 2422.180 kb on + strand at 2422.180 kb on + strand at 2422.180 kb on + strand at 2422.180 kb on + strand at 2422.181 kb on - strand at 2422.181 kb on - strand at 2422.181 kb on - strand at 2422.181 kb on - strand at 2422.181 kb on - strand at 2422.181 kb on - strand at 2422.181 kb on - strand at 2422.181 kb on - strand at 2422.181 kb on - strand at 2422.259 kb on + strand at 2422.259 kb on + strand at 2422.260 kb on - strand at 2422.496 kb on + strand, within Psyr_2077 at 2422.670 kb on + strand, within Psyr_2077 at 2422.769 kb on + strand, within Psyr_2077 at 2422.769 kb on + strand, within Psyr_2077 at 2422.770 kb on - strand, within Psyr_2077 at 2422.770 kb on - strand, within Psyr_2077 at 2422.770 kb on - strand, within Psyr_2077 at 2422.770 kb on - strand, within Psyr_2077 at 2422.877 kb on + strand, within Psyr_2077 at 2422.877 kb on + strand, within Psyr_2077 at 2422.877 kb on + strand, within Psyr_2077 at 2422.878 kb on - strand, within Psyr_2077 at 2422.878 kb on - strand, within Psyr_2077 at 2422.878 kb on - strand, within Psyr_2077 at 2422.878 kb on - strand, within Psyr_2077
Per-strain Table
Position Strand Gene LocusTag Fraction KB with capsaicin 0.125 mg/ml remove 2,420,559 + -1.4 2,420,560 - +0.5 2,420,560 - +0.7 2,420,568 - -0.6 2,420,620 + +1.7 2,420,620 + -0.4 2,420,620 + +1.1 2,420,620 + +0.7 2,420,620 + +0.2 2,420,620 + -0.3 2,420,620 + +0.3 2,420,620 + -0.1 2,420,620 + +0.3 2,420,621 - +0.0 2,420,621 - +0.2 2,420,621 - -0.3 2,420,621 - +0.5 2,420,621 - +0.2 2,420,621 - +0.2 2,420,621 - -0.1 2,420,621 - -1.1 2,420,621 - +0.5 2,420,621 - +0.0 2,420,621 - +0.6 2,420,621 - -0.3 2,420,621 - -2.8 2,420,621 - +0.1 2,420,653 + Psyr_2075 0.12 -1.2 2,420,653 + Psyr_2075 0.12 -0.4 2,420,654 - Psyr_2075 0.12 +2.3 2,420,876 - Psyr_2075 0.37 +0.6 2,420,927 + Psyr_2075 0.42 +0.4 2,420,927 + Psyr_2075 0.42 -0.6 2,420,927 + Psyr_2075 0.42 +1.7 2,420,927 + Psyr_2075 0.42 -0.5 2,420,927 + Psyr_2075 0.42 +0.9 2,420,927 + Psyr_2075 0.42 +0.1 2,420,928 - Psyr_2075 0.42 -1.0 2,420,928 - Psyr_2075 0.42 -1.0 2,421,435 - -0.0 2,421,535 + -0.4 2,421,535 + +0.2 2,421,535 + -1.3 2,421,535 + +1.7 2,421,535 + +0.3 2,421,535 + +0.1 2,421,535 + +1.3 2,421,535 + +0.5 2,421,536 - +0.3 2,421,536 - +1.2 2,421,536 - +0.1 2,421,536 - +0.5 2,421,536 - -0.1 2,421,554 + +0.6 2,421,555 - +0.0 2,421,555 - +1.5 2,421,555 - -0.1 2,421,576 - -0.3 2,421,584 + -0.1 2,421,584 + +0.3 2,421,584 + +1.2 2,421,584 + -0.4 2,421,585 - -1.8 2,421,585 - +0.4 2,421,585 - -0.9 2,421,585 - -0.1 2,421,589 - Psyr_2076 0.11 +0.6 2,421,589 - Psyr_2076 0.11 +0.7 2,421,635 + Psyr_2076 0.20 -0.3 2,421,635 + Psyr_2076 0.20 +0.9 2,421,635 + Psyr_2076 0.20 -0.0 2,421,635 + Psyr_2076 0.20 -0.8 2,421,636 - Psyr_2076 0.20 +0.7 2,421,636 - Psyr_2076 0.20 -0.5 2,421,692 + Psyr_2076 0.32 +0.7 2,421,693 - Psyr_2076 0.32 -0.8 2,421,693 - Psyr_2076 0.32 -0.4 2,421,693 - Psyr_2076 0.32 +0.6 2,421,756 + Psyr_2076 0.44 +2.1 2,421,757 - Psyr_2076 0.45 +1.2 2,421,757 - Psyr_2076 0.45 +0.1 2,421,757 - Psyr_2076 0.45 +0.1 2,421,826 + Psyr_2076 0.59 +0.5 2,421,993 - -1.3 2,421,993 - +0.1 2,421,993 - -1.1 2,422,133 + +0.8 2,422,135 + -1.1 2,422,160 + -0.1 2,422,161 - -1.8 2,422,176 + +0.0 2,422,176 + +1.5 2,422,177 - -2.6 2,422,177 - -0.8 2,422,180 + -0.6 2,422,180 + -0.3 2,422,180 + +0.4 2,422,180 + -0.6 2,422,180 + -0.6 2,422,180 + +0.3 2,422,180 + -0.7 2,422,180 + +1.0 2,422,180 + -1.1 2,422,180 + -1.6 2,422,180 + -1.1 2,422,180 + +0.7 2,422,180 + +0.5 2,422,180 + -1.0 2,422,180 + +1.7 2,422,180 + +0.7 2,422,180 + +3.1 2,422,180 + -1.8 2,422,180 + +1.3 2,422,181 - -0.6 2,422,181 - -0.6 2,422,181 - -3.4 2,422,181 - -0.8 2,422,181 - -0.5 2,422,181 - +1.0 2,422,181 - +0.2 2,422,181 - +0.0 2,422,181 - -0.8 2,422,259 + -0.4 2,422,259 + -1.1 2,422,260 - +0.0 2,422,496 + Psyr_2077 0.32 +0.1 2,422,670 + Psyr_2077 0.50 -0.3 2,422,769 + Psyr_2077 0.60 -0.4 2,422,769 + Psyr_2077 0.60 -0.5 2,422,770 - Psyr_2077 0.60 +1.5 2,422,770 - Psyr_2077 0.60 -0.3 2,422,770 - Psyr_2077 0.60 +3.9 2,422,770 - Psyr_2077 0.60 -0.5 2,422,877 + Psyr_2077 0.71 +1.2 2,422,877 + Psyr_2077 0.71 +0.6 2,422,877 + Psyr_2077 0.71 -0.3 2,422,878 - Psyr_2077 0.71 -1.8 2,422,878 - Psyr_2077 0.71 -2.3 2,422,878 - Psyr_2077 0.71 -0.7 2,422,878 - Psyr_2077 0.71 -1.8
Or see this region's nucleotide sequence