Experiment: KB with capsaicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0579 and Psyr_0580 overlap by 4 nucleotides Psyr_0580 and Psyr_0581 are separated by 257 nucleotides Psyr_0581 and Psyr_0582 are separated by 28 nucleotides Psyr_0582 and Psyr_0583 are separated by 39 nucleotides
Psyr_0579: Psyr_0579 - RNAse R, at 659,938 to 662,571
_0579
Psyr_0580: Psyr_0580 - 23S rRNA Gm-2251 2'-O-methyltransferase, at 662,568 to 663,323
_0580
Psyr_0581: Psyr_0581 - SSU ribosomal protein S6P, at 663,581 to 664,006
_0581
Psyr_0582: Psyr_0582 - SSU ribosomal protein S18P, at 664,035 to 664,265
_0582
Psyr_0583: Psyr_0583 - membrane protein, putative, at 664,305 to 665,201
_0583
Position (kb)
662
663
664 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 661.579 kb on + strand, within Psyr_0579 at 661.580 kb on - strand, within Psyr_0579 at 661.628 kb on - strand, within Psyr_0579 at 661.663 kb on + strand, within Psyr_0579 at 661.672 kb on + strand, within Psyr_0579 at 661.811 kb on - strand, within Psyr_0579 at 661.895 kb on + strand, within Psyr_0579 at 661.918 kb on + strand, within Psyr_0579 at 661.918 kb on + strand, within Psyr_0579 at 661.919 kb on - strand, within Psyr_0579 at 661.919 kb on - strand, within Psyr_0579 at 661.919 kb on - strand, within Psyr_0579 at 661.968 kb on - strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.005 kb on + strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.006 kb on - strand, within Psyr_0579 at 662.047 kb on + strand, within Psyr_0579 at 662.048 kb on - strand, within Psyr_0579 at 662.051 kb on - strand, within Psyr_0579 at 662.227 kb on - strand, within Psyr_0579 at 662.421 kb on + strand at 662.422 kb on - strand at 662.550 kb on + strand at 662.605 kb on + strand at 662.605 kb on + strand at 662.606 kb on - strand at 662.614 kb on + strand at 662.614 kb on + strand at 662.614 kb on + strand at 662.615 kb on - strand at 662.915 kb on + strand, within Psyr_0580 at 662.915 kb on + strand, within Psyr_0580 at 663.051 kb on - strand, within Psyr_0580 at 663.121 kb on - strand, within Psyr_0580 at 663.152 kb on + strand, within Psyr_0580 at 663.207 kb on + strand, within Psyr_0580 at 663.208 kb on - strand, within Psyr_0580 at 663.321 kb on + strand at 663.324 kb on + strand at 663.324 kb on + strand at 663.324 kb on + strand at 663.324 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.353 kb on - strand at 663.353 kb on - strand at 663.353 kb on - strand at 663.353 kb on - strand at 663.359 kb on + strand at 663.359 kb on + strand at 663.359 kb on + strand at 663.359 kb on + strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.376 kb on + strand at 663.376 kb on + strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.382 kb on + strand at 663.383 kb on - strand at 663.383 kb on - strand at 663.421 kb on + strand at 663.422 kb on - strand at 663.422 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with capsaicin 0.125 mg/ml remove 661,579 + Psyr_0579 0.62 -1.5 661,580 - Psyr_0579 0.62 -1.7 661,628 - Psyr_0579 0.64 -1.2 661,663 + Psyr_0579 0.65 -0.5 661,672 + Psyr_0579 0.66 -1.9 661,811 - Psyr_0579 0.71 -2.2 661,895 + Psyr_0579 0.74 +0.1 661,918 + Psyr_0579 0.75 +4.1 661,918 + Psyr_0579 0.75 -1.7 661,919 - Psyr_0579 0.75 +1.1 661,919 - Psyr_0579 0.75 -2.5 661,919 - Psyr_0579 0.75 -1.3 661,968 - Psyr_0579 0.77 -1.7 662,005 + Psyr_0579 0.78 +0.7 662,005 + Psyr_0579 0.78 +1.1 662,005 + Psyr_0579 0.78 +1.8 662,005 + Psyr_0579 0.78 -0.3 662,005 + Psyr_0579 0.78 -1.7 662,005 + Psyr_0579 0.78 +2.7 662,005 + Psyr_0579 0.78 +0.8 662,005 + Psyr_0579 0.78 +0.1 662,005 + Psyr_0579 0.78 -0.9 662,005 + Psyr_0579 0.78 +2.1 662,005 + Psyr_0579 0.78 -0.2 662,006 - Psyr_0579 0.79 -2.6 662,006 - Psyr_0579 0.79 -2.4 662,006 - Psyr_0579 0.79 -1.0 662,006 - Psyr_0579 0.79 -0.6 662,006 - Psyr_0579 0.79 +3.7 662,006 - Psyr_0579 0.79 -0.5 662,006 - Psyr_0579 0.79 +0.7 662,006 - Psyr_0579 0.79 -0.5 662,006 - Psyr_0579 0.79 +0.1 662,006 - Psyr_0579 0.79 -1.3 662,006 - Psyr_0579 0.79 -0.1 662,006 - Psyr_0579 0.79 -3.3 662,006 - Psyr_0579 0.79 -2.3 662,006 - Psyr_0579 0.79 -0.9 662,006 - Psyr_0579 0.79 -1.9 662,047 + Psyr_0579 0.80 +0.9 662,048 - Psyr_0579 0.80 +2.1 662,051 - Psyr_0579 0.80 -0.2 662,227 - Psyr_0579 0.87 +0.4 662,421 + -0.3 662,422 - -2.0 662,550 + -0.5 662,605 + +0.0 662,605 + +0.8 662,606 - +1.1 662,614 + -0.1 662,614 + -0.9 662,614 + +0.5 662,615 - +0.1 662,915 + Psyr_0580 0.46 -1.3 662,915 + Psyr_0580 0.46 +1.4 663,051 - Psyr_0580 0.64 +0.8 663,121 - Psyr_0580 0.73 +1.1 663,152 + Psyr_0580 0.77 +0.4 663,207 + Psyr_0580 0.85 +2.1 663,208 - Psyr_0580 0.85 +1.5 663,321 + -1.0 663,324 + -0.5 663,324 + +0.9 663,324 + +2.7 663,324 + -1.8 663,352 + -0.5 663,352 + +0.4 663,352 + +1.0 663,352 + -1.1 663,352 + -0.9 663,352 + +1.9 663,353 - +0.0 663,353 - +1.1 663,353 - +0.6 663,353 - -0.7 663,359 + +0.1 663,359 + -0.2 663,359 + -0.1 663,359 + +0.8 663,360 - +1.6 663,360 - -1.4 663,360 - +3.1 663,360 - +2.0 663,360 - +0.1 663,360 - -0.6 663,360 - +2.7 663,376 + -0.7 663,376 + -0.5 663,377 - -0.2 663,377 - +0.5 663,377 - +0.1 663,377 - +2.1 663,377 - -0.5 663,382 + +0.3 663,383 - -1.9 663,383 - +1.1 663,421 + +0.0 663,422 - -0.6 663,422 - +1.0
Or see this region's nucleotide sequence