Experiment: KB with capsaicin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0465 and Psyr_0466 are separated by 10 nucleotides Psyr_0466 and Psyr_0467 are separated by 225 nucleotides Psyr_0467 and Psyr_0468 are separated by 173 nucleotides
Psyr_0465: Psyr_0465 - HopAN1 protein, at 503,530 to 504,819
_0465
Psyr_0466: Psyr_0466 - 3-hydroxyisobutyrate dehydrogenase, at 504,830 to 505,735
_0466
Psyr_0467: Psyr_0467 - Protein of unknown function UPF0153, at 505,961 to 506,416
_0467
Psyr_0468: Psyr_0468 - Aminotransferase, class I and II, at 506,590 to 507,798
_0468
Position (kb) 504
505
506 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 503.865 kb on - strand, within Psyr_0465 at 503.865 kb on - strand, within Psyr_0465 at 503.865 kb on - strand, within Psyr_0465 at 503.865 kb on - strand, within Psyr_0465 at 503.873 kb on - strand, within Psyr_0465 at 504.176 kb on + strand, within Psyr_0465 at 504.176 kb on + strand, within Psyr_0465 at 504.177 kb on - strand, within Psyr_0465 at 504.177 kb on - strand, within Psyr_0465 at 504.177 kb on - strand, within Psyr_0465 at 504.177 kb on - strand, within Psyr_0465 at 504.284 kb on + strand, within Psyr_0465 at 504.284 kb on + strand, within Psyr_0465 at 504.548 kb on + strand, within Psyr_0465 at 504.548 kb on + strand, within Psyr_0465 at 504.548 kb on + strand, within Psyr_0465 at 504.548 kb on + strand, within Psyr_0465 at 504.548 kb on + strand, within Psyr_0465 at 504.548 kb on + strand, within Psyr_0465 at 504.549 kb on - strand, within Psyr_0465 at 504.549 kb on - strand, within Psyr_0465 at 504.559 kb on + strand, within Psyr_0465 at 504.559 kb on + strand, within Psyr_0465 at 504.560 kb on - strand, within Psyr_0465 at 504.560 kb on - strand, within Psyr_0465 at 504.560 kb on - strand, within Psyr_0465 at 505.044 kb on + strand, within Psyr_0466 at 505.119 kb on + strand, within Psyr_0466 at 505.119 kb on + strand, within Psyr_0466 at 505.120 kb on - strand, within Psyr_0466 at 505.120 kb on - strand, within Psyr_0466 at 505.120 kb on - strand, within Psyr_0466 at 505.120 kb on - strand, within Psyr_0466 at 505.187 kb on + strand, within Psyr_0466 at 505.187 kb on + strand, within Psyr_0466 at 505.188 kb on - strand, within Psyr_0466 at 505.188 kb on - strand, within Psyr_0466 at 505.254 kb on + strand, within Psyr_0466 at 505.383 kb on + strand, within Psyr_0466 at 505.384 kb on - strand, within Psyr_0466 at 505.536 kb on - strand, within Psyr_0466 at 505.740 kb on + strand at 505.788 kb on + strand at 505.789 kb on - strand at 506.037 kb on + strand, within Psyr_0467 at 506.037 kb on + strand, within Psyr_0467 at 506.037 kb on + strand, within Psyr_0467 at 506.103 kb on + strand, within Psyr_0467 at 506.103 kb on + strand, within Psyr_0467 at 506.103 kb on + strand, within Psyr_0467 at 506.103 kb on + strand, within Psyr_0467 at 506.103 kb on + strand, within Psyr_0467 at 506.103 kb on + strand, within Psyr_0467 at 506.104 kb on - strand, within Psyr_0467 at 506.104 kb on - strand, within Psyr_0467 at 506.104 kb on - strand, within Psyr_0467 at 506.104 kb on - strand, within Psyr_0467 at 506.104 kb on - strand, within Psyr_0467 at 506.104 kb on - strand, within Psyr_0467 at 506.104 kb on - strand, within Psyr_0467 at 506.136 kb on + strand, within Psyr_0467 at 506.137 kb on - strand, within Psyr_0467 at 506.194 kb on - strand, within Psyr_0467 at 506.358 kb on + strand, within Psyr_0467 at 506.358 kb on + strand, within Psyr_0467 at 506.359 kb on - strand, within Psyr_0467 at 506.359 kb on - strand, within Psyr_0467
Per-strain Table
Position Strand Gene LocusTag Fraction KB with capsaicin 0.125 mg/ml remove 503,865 - Psyr_0465 0.26 +0.3 503,865 - Psyr_0465 0.26 -0.5 503,865 - Psyr_0465 0.26 -0.3 503,865 - Psyr_0465 0.26 -0.6 503,873 - Psyr_0465 0.27 +0.6 504,176 + Psyr_0465 0.50 -0.5 504,176 + Psyr_0465 0.50 +0.3 504,177 - Psyr_0465 0.50 -2.5 504,177 - Psyr_0465 0.50 +0.2 504,177 - Psyr_0465 0.50 -0.9 504,177 - Psyr_0465 0.50 +1.0 504,284 + Psyr_0465 0.58 -0.0 504,284 + Psyr_0465 0.58 +0.8 504,548 + Psyr_0465 0.79 -0.4 504,548 + Psyr_0465 0.79 +1.3 504,548 + Psyr_0465 0.79 -0.8 504,548 + Psyr_0465 0.79 -0.5 504,548 + Psyr_0465 0.79 -2.5 504,548 + Psyr_0465 0.79 -0.3 504,549 - Psyr_0465 0.79 +0.0 504,549 - Psyr_0465 0.79 -0.8 504,559 + Psyr_0465 0.80 +0.2 504,559 + Psyr_0465 0.80 -0.9 504,560 - Psyr_0465 0.80 -1.8 504,560 - Psyr_0465 0.80 +0.0 504,560 - Psyr_0465 0.80 +1.3 505,044 + Psyr_0466 0.24 +0.1 505,119 + Psyr_0466 0.32 -1.0 505,119 + Psyr_0466 0.32 -0.1 505,120 - Psyr_0466 0.32 -0.9 505,120 - Psyr_0466 0.32 +1.6 505,120 - Psyr_0466 0.32 +0.6 505,120 - Psyr_0466 0.32 -1.3 505,187 + Psyr_0466 0.39 -0.7 505,187 + Psyr_0466 0.39 +3.3 505,188 - Psyr_0466 0.40 -0.1 505,188 - Psyr_0466 0.40 +2.3 505,254 + Psyr_0466 0.47 -0.5 505,383 + Psyr_0466 0.61 -1.7 505,384 - Psyr_0466 0.61 -0.4 505,536 - Psyr_0466 0.78 -0.8 505,740 + +1.3 505,788 + +1.1 505,789 - -0.3 506,037 + Psyr_0467 0.17 +0.3 506,037 + Psyr_0467 0.17 +0.5 506,037 + Psyr_0467 0.17 -0.2 506,103 + Psyr_0467 0.31 +0.6 506,103 + Psyr_0467 0.31 -0.6 506,103 + Psyr_0467 0.31 -0.3 506,103 + Psyr_0467 0.31 -0.1 506,103 + Psyr_0467 0.31 +0.1 506,103 + Psyr_0467 0.31 +0.2 506,104 - Psyr_0467 0.31 +2.9 506,104 - Psyr_0467 0.31 -1.7 506,104 - Psyr_0467 0.31 -0.5 506,104 - Psyr_0467 0.31 +0.0 506,104 - Psyr_0467 0.31 +0.1 506,104 - Psyr_0467 0.31 -0.2 506,104 - Psyr_0467 0.31 +0.0 506,136 + Psyr_0467 0.38 +0.5 506,137 - Psyr_0467 0.39 +0.0 506,194 - Psyr_0467 0.51 +0.3 506,358 + Psyr_0467 0.87 -0.6 506,358 + Psyr_0467 0.87 -0.6 506,359 - Psyr_0467 0.87 -0.6 506,359 - Psyr_0467 0.87 -0.0
Or see this region's nucleotide sequence