Experiment: H2 N-free with 10mM D-glucosamine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08315 and MMJJ_RS08320 are separated by 28 nucleotides MMJJ_RS08320 and MMJJ_RS08325 are separated by 11 nucleotides MMJJ_RS08325 and MMJJ_RS08330 are separated by 149 nucleotides
MMJJ_RS08315: MMJJ_RS08315 - DNA protection protein DPS, at 1,533,409 to 1,533,960
_RS08315
MMJJ_RS08320: MMJJ_RS08320 - CDP-diacylglycerol--serine O-phosphatidyltransferase, at 1,533,989 to 1,534,603
_RS08320
MMJJ_RS08325: MMJJ_RS08325 - glycosyltransferase family 2 protein, at 1,534,615 to 1,535,307
_RS08325
MMJJ_RS08330: MMJJ_RS08330 - SufD family Fe-S cluster assembly protein, at 1,535,457 to 1,536,683
_RS08330
Position (kb)
1534
1535
1536 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1533.631 kb on - strand, within MMJJ_RS08315 at 1533.632 kb on + strand, within MMJJ_RS08315 at 1533.678 kb on - strand, within MMJJ_RS08315 at 1533.681 kb on + strand, within MMJJ_RS08315 at 1533.701 kb on + strand, within MMJJ_RS08315 at 1533.701 kb on + strand, within MMJJ_RS08315 at 1533.729 kb on - strand, within MMJJ_RS08315 at 1533.730 kb on + strand, within MMJJ_RS08315 at 1533.746 kb on - strand, within MMJJ_RS08315 at 1533.771 kb on - strand, within MMJJ_RS08315 at 1533.823 kb on - strand, within MMJJ_RS08315 at 1533.824 kb on + strand, within MMJJ_RS08315 at 1533.824 kb on + strand, within MMJJ_RS08315 at 1533.846 kb on + strand, within MMJJ_RS08315 at 1533.958 kb on - strand at 1533.967 kb on - strand at 1533.968 kb on + strand at 1533.981 kb on + strand at 1533.991 kb on + strand at 1534.003 kb on - strand at 1534.003 kb on - strand at 1534.007 kb on - strand at 1534.007 kb on - strand at 1534.008 kb on + strand at 1534.019 kb on - strand at 1534.019 kb on - strand at 1534.020 kb on + strand at 1534.024 kb on + strand at 1534.024 kb on + strand at 1534.129 kb on - strand, within MMJJ_RS08320 at 1534.140 kb on + strand, within MMJJ_RS08320 at 1534.140 kb on + strand, within MMJJ_RS08320 at 1534.229 kb on - strand, within MMJJ_RS08320 at 1534.325 kb on - strand, within MMJJ_RS08320 at 1534.553 kb on - strand at 1534.756 kb on - strand, within MMJJ_RS08325 at 1534.782 kb on - strand, within MMJJ_RS08325 at 1534.993 kb on - strand, within MMJJ_RS08325 at 1535.200 kb on - strand, within MMJJ_RS08325 at 1535.200 kb on - strand, within MMJJ_RS08325 at 1535.244 kb on - strand at 1535.268 kb on - strand at 1535.344 kb on - strand at 1535.357 kb on - strand at 1535.357 kb on - strand at 1535.357 kb on - strand at 1535.370 kb on - strand at 1535.370 kb on - strand at 1535.372 kb on - strand at 1535.383 kb on + strand at 1535.386 kb on + strand at 1535.386 kb on + strand at 1535.386 kb on + strand at 1535.386 kb on + strand at 1535.389 kb on - strand at 1535.406 kb on - strand at 1535.426 kb on - strand at 1535.427 kb on + strand at 1535.458 kb on - strand at 1535.599 kb on + strand, within MMJJ_RS08330 at 1535.764 kb on + strand, within MMJJ_RS08330 at 1535.783 kb on - strand, within MMJJ_RS08330 at 1535.872 kb on + strand, within MMJJ_RS08330 at 1535.899 kb on + strand, within MMJJ_RS08330 at 1536.153 kb on + strand, within MMJJ_RS08330 at 1536.156 kb on + strand, within MMJJ_RS08330
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM D-glucosamine remove 1,533,631 - MMJJ_RS08315 0.40 +1.0 1,533,632 + MMJJ_RS08315 0.40 -1.4 1,533,678 - MMJJ_RS08315 0.49 -0.2 1,533,681 + MMJJ_RS08315 0.49 -0.3 1,533,701 + MMJJ_RS08315 0.53 +1.2 1,533,701 + MMJJ_RS08315 0.53 -0.9 1,533,729 - MMJJ_RS08315 0.58 -0.4 1,533,730 + MMJJ_RS08315 0.58 +0.5 1,533,746 - MMJJ_RS08315 0.61 +0.8 1,533,771 - MMJJ_RS08315 0.66 +0.2 1,533,823 - MMJJ_RS08315 0.75 +0.5 1,533,824 + MMJJ_RS08315 0.75 -0.5 1,533,824 + MMJJ_RS08315 0.75 -1.8 1,533,846 + MMJJ_RS08315 0.79 +1.0 1,533,958 - +0.3 1,533,967 - +0.4 1,533,968 + -0.1 1,533,981 + +0.8 1,533,991 + +4.5 1,534,003 - +0.0 1,534,003 - +0.1 1,534,007 - -3.1 1,534,007 - -0.4 1,534,008 + -0.1 1,534,019 - -0.9 1,534,019 - +0.3 1,534,020 + -2.1 1,534,024 + -3.2 1,534,024 + +0.1 1,534,129 - MMJJ_RS08320 0.23 -1.4 1,534,140 + MMJJ_RS08320 0.25 -1.6 1,534,140 + MMJJ_RS08320 0.25 -1.1 1,534,229 - MMJJ_RS08320 0.39 -2.2 1,534,325 - MMJJ_RS08320 0.55 +1.1 1,534,553 - +0.1 1,534,756 - MMJJ_RS08325 0.20 +0.1 1,534,782 - MMJJ_RS08325 0.24 -0.2 1,534,993 - MMJJ_RS08325 0.55 +0.1 1,535,200 - MMJJ_RS08325 0.84 -1.6 1,535,200 - MMJJ_RS08325 0.84 -0.2 1,535,244 - -1.9 1,535,268 - +0.5 1,535,344 - -1.3 1,535,357 - +1.1 1,535,357 - -0.2 1,535,357 - -0.9 1,535,370 - -0.4 1,535,370 - -0.9 1,535,372 - +1.1 1,535,383 + -0.9 1,535,386 + -0.0 1,535,386 + +1.3 1,535,386 + +1.1 1,535,386 + +1.7 1,535,389 - +0.3 1,535,406 - -0.8 1,535,426 - -0.1 1,535,427 + -1.4 1,535,458 - -0.7 1,535,599 + MMJJ_RS08330 0.12 +0.3 1,535,764 + MMJJ_RS08330 0.25 -0.9 1,535,783 - MMJJ_RS08330 0.27 -2.1 1,535,872 + MMJJ_RS08330 0.34 -0.7 1,535,899 + MMJJ_RS08330 0.36 -2.2 1,536,153 + MMJJ_RS08330 0.57 -0.2 1,536,156 + MMJJ_RS08330 0.57 -2.0
Or see this region's nucleotide sequence