Experiment: H2 N-free with 10mM D-glucosamine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05025 and MMJJ_RS05030 are separated by 146 nucleotides MMJJ_RS05030 and MMJJ_RS05035 are separated by 194 nucleotides
MMJJ_RS05025: MMJJ_RS05025 - glutamine amidotransferase family protein, at 934,056 to 935,147
_RS05025
MMJJ_RS05030: MMJJ_RS05030 - orotate phosphoribosyltransferase-like protein, at 935,294 to 935,911
_RS05030
MMJJ_RS05035: MMJJ_RS05035 - 4Fe-4S binding protein, at 936,106 to 936,951
_RS05035
Position (kb) 935
936 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 934.547 kb on - strand, within MMJJ_RS05025 at 934.631 kb on + strand at 934.955 kb on - strand at 935.211 kb on - strand at 935.224 kb on + strand at 935.224 kb on + strand at 935.225 kb on - strand at 935.226 kb on + strand at 935.226 kb on + strand at 935.226 kb on + strand at 935.226 kb on + strand at 935.248 kb on + strand at 935.261 kb on - strand at 935.262 kb on + strand at 935.262 kb on + strand at 935.262 kb on + strand at 935.274 kb on + strand at 935.302 kb on - strand at 935.302 kb on - strand at 935.306 kb on + strand at 935.324 kb on + strand at 935.336 kb on - strand at 935.337 kb on + strand at 935.337 kb on + strand at 935.387 kb on - strand, within MMJJ_RS05030 at 935.418 kb on - strand, within MMJJ_RS05030 at 935.423 kb on - strand, within MMJJ_RS05030 at 935.457 kb on + strand, within MMJJ_RS05030 at 935.562 kb on - strand, within MMJJ_RS05030 at 935.562 kb on - strand, within MMJJ_RS05030 at 935.562 kb on - strand, within MMJJ_RS05030 at 935.562 kb on - strand, within MMJJ_RS05030 at 935.562 kb on - strand, within MMJJ_RS05030 at 935.562 kb on - strand, within MMJJ_RS05030 at 935.562 kb on - strand at 935.562 kb on - strand, within MMJJ_RS05030 at 935.562 kb on - strand, within MMJJ_RS05030 at 935.562 kb on - strand, within MMJJ_RS05030 at 935.562 kb on - strand, within MMJJ_RS05030 at 935.562 kb on - strand, within MMJJ_RS05030 at 935.563 kb on + strand, within MMJJ_RS05030 at 935.563 kb on + strand, within MMJJ_RS05030 at 935.563 kb on + strand, within MMJJ_RS05030 at 935.563 kb on + strand, within MMJJ_RS05030 at 935.563 kb on + strand, within MMJJ_RS05030 at 935.563 kb on + strand, within MMJJ_RS05030 at 935.564 kb on - strand, within MMJJ_RS05030 at 935.564 kb on - strand, within MMJJ_RS05030 at 935.564 kb on - strand, within MMJJ_RS05030 at 935.564 kb on - strand, within MMJJ_RS05030 at 935.564 kb on - strand, within MMJJ_RS05030 at 935.565 kb on + strand, within MMJJ_RS05030 at 935.565 kb on + strand, within MMJJ_RS05030 at 935.565 kb on + strand, within MMJJ_RS05030 at 935.565 kb on + strand, within MMJJ_RS05030 at 935.566 kb on - strand at 935.686 kb on - strand, within MMJJ_RS05030 at 935.798 kb on - strand, within MMJJ_RS05030 at 935.798 kb on - strand, within MMJJ_RS05030 at 935.798 kb on - strand, within MMJJ_RS05030 at 935.799 kb on + strand, within MMJJ_RS05030 at 935.803 kb on - strand, within MMJJ_RS05030 at 935.804 kb on + strand, within MMJJ_RS05030 at 935.808 kb on - strand, within MMJJ_RS05030 at 935.808 kb on - strand, within MMJJ_RS05030 at 935.808 kb on - strand, within MMJJ_RS05030 at 935.809 kb on + strand, within MMJJ_RS05030 at 935.809 kb on + strand, within MMJJ_RS05030 at 935.809 kb on + strand, within MMJJ_RS05030 at 935.810 kb on - strand, within MMJJ_RS05030 at 935.829 kb on + strand, within MMJJ_RS05030 at 935.829 kb on + strand, within MMJJ_RS05030 at 935.890 kb on - strand at 935.894 kb on - strand at 935.895 kb on + strand at 935.932 kb on - strand at 935.932 kb on - strand at 935.932 kb on - strand at 935.935 kb on - strand at 935.943 kb on - strand at 935.943 kb on - strand at 935.943 kb on - strand at 935.943 kb on - strand at 935.944 kb on + strand at 935.944 kb on + strand at 935.944 kb on + strand at 935.945 kb on - strand at 935.948 kb on - strand at 935.948 kb on - strand at 935.949 kb on + strand at 935.954 kb on - strand at 935.956 kb on - strand at 935.993 kb on - strand at 935.993 kb on - strand at 935.993 kb on - strand at 935.993 kb on - strand at 935.993 kb on - strand at 935.993 kb on - strand at 935.993 kb on - strand at 935.993 kb on - strand at 935.993 kb on - strand at 935.993 kb on - strand at 935.993 kb on - strand at 935.993 kb on - strand at 935.993 kb on - strand at 935.993 kb on - strand at 935.993 kb on - strand at 935.994 kb on + strand at 935.994 kb on + strand at 935.994 kb on + strand at 935.994 kb on + strand at 935.994 kb on + strand at 935.994 kb on + strand at 935.994 kb on + strand at 935.994 kb on + strand at 935.994 kb on + strand at 935.994 kb on + strand at 935.994 kb on + strand at 935.995 kb on - strand at 935.995 kb on - strand at 935.995 kb on - strand at 935.995 kb on - strand at 935.995 kb on - strand at 935.996 kb on + strand at 935.996 kb on + strand at 935.996 kb on + strand at 935.996 kb on + strand at 935.996 kb on + strand at 936.016 kb on - strand at 936.029 kb on - strand at 936.029 kb on - strand at 936.030 kb on + strand at 936.038 kb on + strand at 936.055 kb on - strand at 936.057 kb on - strand at 936.058 kb on + strand at 936.058 kb on + strand at 936.058 kb on + strand at 936.066 kb on + strand at 936.074 kb on - strand at 936.077 kb on - strand at 936.077 kb on - strand at 936.088 kb on + strand at 936.088 kb on + strand at 936.090 kb on - strand at 936.090 kb on - strand at 936.091 kb on + strand at 936.095 kb on - strand at 936.096 kb on + strand at 936.096 kb on + strand at 936.096 kb on + strand at 936.103 kb on + strand at 936.105 kb on - strand at 936.106 kb on + strand at 936.122 kb on - strand at 936.123 kb on + strand at 936.127 kb on + strand at 936.127 kb on + strand at 936.256 kb on + strand, within MMJJ_RS05035 at 936.263 kb on + strand, within MMJJ_RS05035 at 936.263 kb on + strand, within MMJJ_RS05035 at 936.265 kb on + strand, within MMJJ_RS05035 at 936.321 kb on + strand, within MMJJ_RS05035 at 936.329 kb on - strand, within MMJJ_RS05035 at 936.492 kb on - strand, within MMJJ_RS05035 at 936.498 kb on - strand, within MMJJ_RS05035 at 936.525 kb on - strand, within MMJJ_RS05035 at 936.525 kb on - strand, within MMJJ_RS05035 at 936.526 kb on + strand, within MMJJ_RS05035 at 936.554 kb on - strand, within MMJJ_RS05035 at 936.562 kb on + strand, within MMJJ_RS05035 at 936.576 kb on + strand, within MMJJ_RS05035 at 936.672 kb on + strand, within MMJJ_RS05035 at 936.672 kb on + strand, within MMJJ_RS05035 at 936.690 kb on - strand, within MMJJ_RS05035 at 936.750 kb on + strand, within MMJJ_RS05035 at 936.812 kb on + strand, within MMJJ_RS05035 at 936.836 kb on - strand, within MMJJ_RS05035
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM D-glucosamine remove 934,547 - MMJJ_RS05025 0.45 -1.3 934,631 + +0.3 934,955 - -0.4 935,211 - -0.5 935,224 + +0.0 935,224 + -1.3 935,225 - +0.3 935,226 + +0.4 935,226 + -0.3 935,226 + -0.7 935,226 + -0.2 935,248 + -1.1 935,261 - -0.8 935,262 + +0.2 935,262 + -0.9 935,262 + -0.3 935,274 + -0.3 935,302 - -0.3 935,302 - +0.1 935,306 + -2.0 935,324 + -3.0 935,336 - -0.6 935,337 + -2.1 935,337 + -3.1 935,387 - MMJJ_RS05030 0.15 -3.0 935,418 - MMJJ_RS05030 0.20 -1.4 935,423 - MMJJ_RS05030 0.21 -4.5 935,457 + MMJJ_RS05030 0.26 -2.7 935,562 - MMJJ_RS05030 0.43 -4.1 935,562 - MMJJ_RS05030 0.43 -1.0 935,562 - MMJJ_RS05030 0.43 -3.5 935,562 - MMJJ_RS05030 0.43 -0.1 935,562 - MMJJ_RS05030 0.43 -2.5 935,562 - MMJJ_RS05030 0.43 +0.3 935,562 - +1.9 935,562 - MMJJ_RS05030 0.43 -2.3 935,562 - MMJJ_RS05030 0.43 -2.1 935,562 - MMJJ_RS05030 0.43 -2.8 935,562 - MMJJ_RS05030 0.43 -3.7 935,562 - MMJJ_RS05030 0.43 -2.4 935,563 + MMJJ_RS05030 0.44 -2.3 935,563 + MMJJ_RS05030 0.44 -3.6 935,563 + MMJJ_RS05030 0.44 -0.3 935,563 + MMJJ_RS05030 0.44 -3.1 935,563 + MMJJ_RS05030 0.44 -1.5 935,563 + MMJJ_RS05030 0.44 -1.9 935,564 - MMJJ_RS05030 0.44 -3.9 935,564 - MMJJ_RS05030 0.44 -1.3 935,564 - MMJJ_RS05030 0.44 -2.2 935,564 - MMJJ_RS05030 0.44 -0.9 935,564 - MMJJ_RS05030 0.44 -1.6 935,565 + MMJJ_RS05030 0.44 -3.6 935,565 + MMJJ_RS05030 0.44 -1.5 935,565 + MMJJ_RS05030 0.44 -4.2 935,565 + MMJJ_RS05030 0.44 -1.7 935,566 - -0.4 935,686 - MMJJ_RS05030 0.63 -2.5 935,798 - MMJJ_RS05030 0.82 -0.9 935,798 - MMJJ_RS05030 0.82 -3.3 935,798 - MMJJ_RS05030 0.82 -1.9 935,799 + MMJJ_RS05030 0.82 -2.6 935,803 - MMJJ_RS05030 0.82 -1.3 935,804 + MMJJ_RS05030 0.83 -0.9 935,808 - MMJJ_RS05030 0.83 -1.9 935,808 - MMJJ_RS05030 0.83 -3.3 935,808 - MMJJ_RS05030 0.83 -3.0 935,809 + MMJJ_RS05030 0.83 -1.4 935,809 + MMJJ_RS05030 0.83 -1.8 935,809 + MMJJ_RS05030 0.83 -3.5 935,810 - MMJJ_RS05030 0.83 -2.8 935,829 + MMJJ_RS05030 0.87 -3.9 935,829 + MMJJ_RS05030 0.87 -1.2 935,890 - -0.8 935,894 - -3.3 935,895 + -3.1 935,932 - -2.7 935,932 - -3.1 935,932 - -1.9 935,935 - -1.3 935,943 - -1.6 935,943 - -2.2 935,943 - -1.1 935,943 - -1.1 935,944 + -0.4 935,944 + -3.0 935,944 + -1.2 935,945 - -1.2 935,948 - +0.9 935,948 - -0.9 935,949 + +0.2 935,954 - -0.8 935,956 - +0.6 935,993 - +0.6 935,993 - +0.7 935,993 - -0.7 935,993 - +0.6 935,993 - -0.0 935,993 - +0.8 935,993 - +1.7 935,993 - +0.1 935,993 - -0.9 935,993 - -0.3 935,993 - +1.1 935,993 - -1.2 935,993 - +0.5 935,993 - -1.6 935,993 - -0.3 935,994 + +0.1 935,994 + +0.6 935,994 + -1.2 935,994 + -0.4 935,994 + -1.0 935,994 + -0.2 935,994 + +0.2 935,994 + -1.3 935,994 + -0.6 935,994 + +0.8 935,994 + +1.2 935,995 - -0.5 935,995 - -0.3 935,995 - +0.6 935,995 - -0.4 935,995 - -0.0 935,996 + +0.6 935,996 + +0.6 935,996 + +0.4 935,996 + +0.6 935,996 + -1.3 936,016 - +0.2 936,029 - +1.3 936,029 - -0.7 936,030 + -0.5 936,038 + +0.5 936,055 - -0.5 936,057 - -1.0 936,058 + -0.4 936,058 + +0.9 936,058 + +3.3 936,066 + -1.4 936,074 - +1.7 936,077 - +1.4 936,077 - +0.6 936,088 + -0.4 936,088 + +0.5 936,090 - +0.3 936,090 - -0.6 936,091 + +0.3 936,095 - +0.1 936,096 + -1.8 936,096 + -0.2 936,096 + +0.2 936,103 + +0.5 936,105 - -0.3 936,106 + -0.9 936,122 - -0.3 936,123 + +1.4 936,127 + +0.9 936,127 + +0.6 936,256 + MMJJ_RS05035 0.18 +0.6 936,263 + MMJJ_RS05035 0.19 +0.6 936,263 + MMJJ_RS05035 0.19 -0.3 936,265 + MMJJ_RS05035 0.19 +0.9 936,321 + MMJJ_RS05035 0.25 -0.1 936,329 - MMJJ_RS05035 0.26 +0.3 936,492 - MMJJ_RS05035 0.46 +0.7 936,498 - MMJJ_RS05035 0.46 -0.7 936,525 - MMJJ_RS05035 0.50 -0.0 936,525 - MMJJ_RS05035 0.50 +0.6 936,526 + MMJJ_RS05035 0.50 +0.4 936,554 - MMJJ_RS05035 0.53 +0.4 936,562 + MMJJ_RS05035 0.54 -0.9 936,576 + MMJJ_RS05035 0.56 -1.0 936,672 + MMJJ_RS05035 0.67 +0.9 936,672 + MMJJ_RS05035 0.67 +0.5 936,690 - MMJJ_RS05035 0.69 +0.7 936,750 + MMJJ_RS05035 0.76 -0.9 936,812 + MMJJ_RS05035 0.83 +0.0 936,836 - MMJJ_RS05035 0.86 -0.1
Or see this region's nucleotide sequence