Experiment: H2 N-free with 10mM D-glucosamine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03960 and MMJJ_RS03965 overlap by 4 nucleotides MMJJ_RS03965 and MMJJ_RS03970 are separated by 178 nucleotides MMJJ_RS03970 and MMJJ_RS03975 are separated by 13 nucleotides
MMJJ_RS03960: MMJJ_RS03960 - DUF2067 domain-containing protein, at 739,052 to 739,669
_RS03960
MMJJ_RS03965: MMJJ_RS03965 - DNA-directed RNA polymerase subunit L, at 739,666 to 739,956
_RS03965
MMJJ_RS03970: MMJJ_RS03970 - 50S ribosomal protein L1, at 740,135 to 740,776
_RS03970
MMJJ_RS03975: MMJJ_RS03975 - 50S ribosomal protein L10, at 740,790 to 741,797
_RS03975
Position (kb) 739
740 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 738.714 kb on - strand at 738.740 kb on + strand at 738.740 kb on + strand at 738.753 kb on - strand at 738.753 kb on - strand at 738.753 kb on - strand at 738.779 kb on - strand at 738.785 kb on - strand at 738.785 kb on - strand at 738.785 kb on - strand at 738.786 kb on + strand at 738.786 kb on + strand at 738.786 kb on + strand at 738.796 kb on - strand at 738.796 kb on - strand at 738.800 kb on - strand at 738.818 kb on - strand at 738.869 kb on - strand at 738.869 kb on - strand at 738.870 kb on + strand at 738.870 kb on + strand at 738.870 kb on + strand at 738.871 kb on - strand at 738.871 kb on - strand at 738.874 kb on + strand at 738.874 kb on + strand at 738.874 kb on + strand at 738.874 kb on + strand at 738.874 kb on + strand at 738.918 kb on - strand at 738.950 kb on - strand at 738.950 kb on - strand at 738.950 kb on - strand at 738.950 kb on - strand at 738.950 kb on - strand at 738.950 kb on - strand at 738.950 kb on - strand at 738.950 kb on - strand at 738.951 kb on + strand at 738.951 kb on + strand at 738.951 kb on + strand at 738.951 kb on + strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.956 kb on + strand at 738.956 kb on + strand at 738.956 kb on + strand at 738.956 kb on + strand at 738.956 kb on + strand at 738.956 kb on + strand at 738.956 kb on + strand at 738.956 kb on + strand at 738.956 kb on + strand at 738.956 kb on + strand at 738.957 kb on - strand at 738.958 kb on + strand at 738.958 kb on + strand at 738.961 kb on - strand at 738.961 kb on - strand at 738.961 kb on - strand at 738.961 kb on - strand at 738.961 kb on - strand at 738.961 kb on - strand at 738.961 kb on - strand at 738.961 kb on - strand at 738.962 kb on + strand at 738.962 kb on + strand at 738.962 kb on + strand at 738.962 kb on + strand at 738.962 kb on + strand at 738.962 kb on + strand at 738.965 kb on - strand at 738.965 kb on - strand at 738.965 kb on - strand at 738.965 kb on - strand at 738.966 kb on + strand at 738.966 kb on + strand at 738.966 kb on + strand at 738.966 kb on + strand at 738.971 kb on - strand at 738.971 kb on - strand at 738.971 kb on - strand at 738.972 kb on + strand at 738.972 kb on + strand at 738.972 kb on + strand at 738.972 kb on + strand at 738.972 kb on + strand at 738.972 kb on + strand at 738.976 kb on + strand at 738.976 kb on + strand at 738.976 kb on + strand at 738.976 kb on + strand at 738.976 kb on + strand at 738.987 kb on - strand at 738.988 kb on + strand at 739.004 kb on - strand at 739.004 kb on - strand at 739.004 kb on - strand at 739.004 kb on - strand at 739.004 kb on - strand at 739.004 kb on - strand at 739.004 kb on - strand at 739.004 kb on - strand at 739.004 kb on - strand at 739.005 kb on + strand at 739.005 kb on + strand at 739.007 kb on + strand at 739.007 kb on + strand at 739.007 kb on + strand at 739.010 kb on - strand at 739.010 kb on - strand at 739.011 kb on + strand at 739.011 kb on + strand at 739.062 kb on - strand at 739.063 kb on + strand at 739.063 kb on + strand at 739.066 kb on + strand at 739.066 kb on + strand at 739.066 kb on + strand at 739.072 kb on + strand at 739.113 kb on - strand at 739.117 kb on - strand, within MMJJ_RS03960 at 739.117 kb on - strand, within MMJJ_RS03960 at 739.118 kb on + strand, within MMJJ_RS03960 at 739.118 kb on + strand, within MMJJ_RS03960 at 739.175 kb on - strand, within MMJJ_RS03960 at 739.176 kb on + strand, within MMJJ_RS03960 at 739.178 kb on + strand, within MMJJ_RS03960 at 739.178 kb on + strand, within MMJJ_RS03960 at 739.198 kb on + strand, within MMJJ_RS03960 at 739.224 kb on - strand, within MMJJ_RS03960 at 739.246 kb on - strand, within MMJJ_RS03960 at 739.246 kb on - strand, within MMJJ_RS03960 at 739.246 kb on - strand, within MMJJ_RS03960 at 739.247 kb on + strand, within MMJJ_RS03960 at 739.247 kb on + strand, within MMJJ_RS03960 at 739.250 kb on + strand, within MMJJ_RS03960 at 739.253 kb on + strand, within MMJJ_RS03960 at 739.254 kb on - strand, within MMJJ_RS03960 at 739.255 kb on + strand, within MMJJ_RS03960 at 739.255 kb on + strand, within MMJJ_RS03960 at 739.258 kb on - strand, within MMJJ_RS03960 at 739.258 kb on - strand, within MMJJ_RS03960 at 739.271 kb on + strand, within MMJJ_RS03960 at 739.271 kb on + strand, within MMJJ_RS03960 at 739.320 kb on - strand, within MMJJ_RS03960 at 739.326 kb on + strand, within MMJJ_RS03960 at 739.368 kb on - strand, within MMJJ_RS03960 at 739.382 kb on + strand, within MMJJ_RS03960 at 739.390 kb on + strand, within MMJJ_RS03960 at 739.436 kb on + strand, within MMJJ_RS03960 at 739.436 kb on + strand, within MMJJ_RS03960 at 739.466 kb on - strand, within MMJJ_RS03960 at 739.467 kb on + strand, within MMJJ_RS03960 at 739.467 kb on + strand, within MMJJ_RS03960 at 739.531 kb on + strand, within MMJJ_RS03960 at 739.531 kb on + strand, within MMJJ_RS03960 at 739.532 kb on - strand, within MMJJ_RS03960 at 739.532 kb on - strand, within MMJJ_RS03960 at 739.532 kb on - strand, within MMJJ_RS03960 at 739.532 kb on - strand, within MMJJ_RS03960 at 739.533 kb on + strand, within MMJJ_RS03960 at 739.533 kb on + strand, within MMJJ_RS03960 at 739.534 kb on - strand, within MMJJ_RS03960 at 739.536 kb on - strand, within MMJJ_RS03960 at 739.537 kb on + strand, within MMJJ_RS03960 at 739.537 kb on + strand, within MMJJ_RS03960 at 739.629 kb on - strand at 739.672 kb on - strand at 739.673 kb on + strand at 739.719 kb on + strand, within MMJJ_RS03965 at 739.719 kb on + strand, within MMJJ_RS03965 at 739.795 kb on - strand at 739.803 kb on + strand, within MMJJ_RS03965 at 739.952 kb on - strand at 739.954 kb on - strand at 739.967 kb on + strand at 739.969 kb on + strand at 739.976 kb on - strand at 739.977 kb on + strand at 740.053 kb on - strand at 740.054 kb on + strand at 740.057 kb on - strand at 740.057 kb on - strand at 740.058 kb on + strand at 740.075 kb on - strand at 740.120 kb on + strand at 740.515 kb on - strand, within MMJJ_RS03970 at 740.551 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM D-glucosamine remove 738,714 - +1.5 738,740 + -0.6 738,740 + -2.2 738,753 - +1.3 738,753 - -0.1 738,753 - +0.7 738,779 - +0.8 738,785 - +1.6 738,785 - +0.8 738,785 - -0.0 738,786 + -0.3 738,786 + -1.1 738,786 + +0.8 738,796 - -2.4 738,796 - +0.3 738,800 - -0.2 738,818 - -0.3 738,869 - +0.1 738,869 - -0.4 738,870 + -0.4 738,870 + -0.2 738,870 + -0.3 738,871 - +0.3 738,871 - -3.0 738,874 + +0.8 738,874 + +0.3 738,874 + -0.2 738,874 + +0.7 738,874 + +1.5 738,918 - -0.2 738,950 - +0.4 738,950 - -0.4 738,950 - -0.6 738,950 - +0.8 738,950 - -2.3 738,950 - -0.9 738,950 - +0.5 738,950 - +1.7 738,951 + +1.0 738,951 + -0.1 738,951 + +1.0 738,951 + -1.4 738,955 - -1.3 738,955 - -0.6 738,955 - -1.6 738,955 - +0.2 738,955 - -0.1 738,955 - -1.0 738,955 - +0.8 738,956 + -1.3 738,956 + +1.2 738,956 + -0.7 738,956 + -0.6 738,956 + -2.7 738,956 + -1.0 738,956 + -1.4 738,956 + -0.6 738,956 + +1.1 738,956 + +0.3 738,957 - +0.8 738,958 + +0.4 738,958 + +1.1 738,961 - -0.8 738,961 - -0.9 738,961 - +0.1 738,961 - +0.5 738,961 - -1.2 738,961 - +0.3 738,961 - -0.3 738,961 - +0.6 738,962 + +0.7 738,962 + +0.1 738,962 + +0.5 738,962 + +0.8 738,962 + +1.2 738,962 + -0.2 738,965 - -1.7 738,965 - +0.4 738,965 - +1.4 738,965 - +0.3 738,966 + +1.4 738,966 + -0.5 738,966 + +0.9 738,966 + +1.9 738,971 - -1.9 738,971 - -2.2 738,971 - +1.8 738,972 + -0.3 738,972 + -0.8 738,972 + +0.1 738,972 + +0.1 738,972 + -0.3 738,972 + +0.5 738,976 + -0.1 738,976 + +1.3 738,976 + -3.3 738,976 + +0.9 738,976 + +0.1 738,987 - -0.1 738,988 + +2.0 739,004 - +0.9 739,004 - -0.7 739,004 - +0.6 739,004 - +0.4 739,004 - -1.2 739,004 - +0.3 739,004 - +0.6 739,004 - -0.1 739,004 - -0.7 739,005 + +2.0 739,005 + -0.3 739,007 + -0.2 739,007 + -0.9 739,007 + +0.3 739,010 - +0.3 739,010 - -1.6 739,011 + -0.5 739,011 + -2.7 739,062 - -0.9 739,063 + -1.4 739,063 + +1.1 739,066 + +0.2 739,066 + +0.5 739,066 + +0.9 739,072 + -0.5 739,113 - +0.4 739,117 - MMJJ_RS03960 0.11 +0.9 739,117 - MMJJ_RS03960 0.11 -1.1 739,118 + MMJJ_RS03960 0.11 +2.1 739,118 + MMJJ_RS03960 0.11 +0.1 739,175 - MMJJ_RS03960 0.20 +0.6 739,176 + MMJJ_RS03960 0.20 +0.5 739,178 + MMJJ_RS03960 0.20 -0.4 739,178 + MMJJ_RS03960 0.20 +2.8 739,198 + MMJJ_RS03960 0.24 +0.4 739,224 - MMJJ_RS03960 0.28 -0.3 739,246 - MMJJ_RS03960 0.31 -0.1 739,246 - MMJJ_RS03960 0.31 +1.7 739,246 - MMJJ_RS03960 0.31 +0.1 739,247 + MMJJ_RS03960 0.32 +0.1 739,247 + MMJJ_RS03960 0.32 +0.7 739,250 + MMJJ_RS03960 0.32 +1.9 739,253 + MMJJ_RS03960 0.33 +0.4 739,254 - MMJJ_RS03960 0.33 +0.8 739,255 + MMJJ_RS03960 0.33 +1.0 739,255 + MMJJ_RS03960 0.33 +0.9 739,258 - MMJJ_RS03960 0.33 -0.6 739,258 - MMJJ_RS03960 0.33 -1.1 739,271 + MMJJ_RS03960 0.35 +2.1 739,271 + MMJJ_RS03960 0.35 -0.7 739,320 - MMJJ_RS03960 0.43 +1.0 739,326 + MMJJ_RS03960 0.44 +1.9 739,368 - MMJJ_RS03960 0.51 -0.1 739,382 + MMJJ_RS03960 0.53 +0.4 739,390 + MMJJ_RS03960 0.55 +0.4 739,436 + MMJJ_RS03960 0.62 -1.2 739,436 + MMJJ_RS03960 0.62 +0.5 739,466 - MMJJ_RS03960 0.67 +0.6 739,467 + MMJJ_RS03960 0.67 +0.2 739,467 + MMJJ_RS03960 0.67 -0.0 739,531 + MMJJ_RS03960 0.78 +0.5 739,531 + MMJJ_RS03960 0.78 +0.6 739,532 - MMJJ_RS03960 0.78 -2.1 739,532 - MMJJ_RS03960 0.78 +0.9 739,532 - MMJJ_RS03960 0.78 -0.4 739,532 - MMJJ_RS03960 0.78 -1.3 739,533 + MMJJ_RS03960 0.78 +0.3 739,533 + MMJJ_RS03960 0.78 -1.4 739,534 - MMJJ_RS03960 0.78 +0.9 739,536 - MMJJ_RS03960 0.78 +0.2 739,537 + MMJJ_RS03960 0.78 +0.0 739,537 + MMJJ_RS03960 0.78 -1.0 739,629 - -1.0 739,672 - -0.3 739,673 + -1.2 739,719 + MMJJ_RS03965 0.18 -0.4 739,719 + MMJJ_RS03965 0.18 -1.4 739,795 - -0.7 739,803 + MMJJ_RS03965 0.47 -1.1 739,952 - +0.5 739,954 - +1.0 739,967 + -1.4 739,969 + +0.9 739,976 - -1.3 739,977 + -1.5 740,053 - -2.8 740,054 + -3.9 740,057 - -1.1 740,057 - -0.1 740,058 + -0.4 740,075 - -0.4 740,120 + -0.9 740,515 - MMJJ_RS03970 0.59 -2.5 740,551 + -1.1
Or see this region's nucleotide sequence