Experiment: H2 N-free with 10mM D-glucosamine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03845 and MMJJ_RS03850 are separated by 159 nucleotides MMJJ_RS03850 and MMJJ_RS03855 are separated by 199 nucleotides MMJJ_RS03855 and MMJJ_RS03860 are separated by 1 nucleotides
MMJJ_RS03845: MMJJ_RS03845 - translation initiation factor IF-2, at 715,305 to 717,101
_RS03845
MMJJ_RS03850: MMJJ_RS03850 - nucleoside-diphosphate kinase, at 717,261 to 717,680
_RS03850
MMJJ_RS03855: MMJJ_RS03855 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 717,880 to 718,356
_RS03855
MMJJ_RS03860: MMJJ_RS03860 - PINc/VapC family ATPase, at 718,358 to 720,250
_RS03860
Position (kb)
717
718 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 716.712 kb on + strand, within MMJJ_RS03845 at 716.754 kb on + strand at 717.036 kb on - strand at 717.115 kb on - strand at 717.121 kb on - strand at 717.158 kb on + strand at 717.159 kb on - strand at 717.159 kb on - strand at 717.196 kb on - strand at 717.204 kb on + strand at 717.207 kb on - strand at 717.208 kb on + strand at 717.208 kb on + strand at 717.208 kb on + strand at 717.212 kb on - strand at 717.231 kb on + strand at 717.255 kb on - strand at 717.282 kb on - strand at 717.303 kb on - strand, within MMJJ_RS03850 at 717.304 kb on + strand, within MMJJ_RS03850 at 717.324 kb on - strand, within MMJJ_RS03850 at 717.324 kb on - strand, within MMJJ_RS03850 at 717.326 kb on - strand, within MMJJ_RS03850 at 717.340 kb on - strand, within MMJJ_RS03850 at 717.340 kb on - strand, within MMJJ_RS03850 at 717.341 kb on + strand, within MMJJ_RS03850 at 717.342 kb on - strand, within MMJJ_RS03850 at 717.392 kb on - strand, within MMJJ_RS03850 at 717.392 kb on - strand, within MMJJ_RS03850 at 717.392 kb on - strand, within MMJJ_RS03850 at 717.393 kb on + strand, within MMJJ_RS03850 at 717.393 kb on + strand, within MMJJ_RS03850 at 717.483 kb on - strand, within MMJJ_RS03850 at 717.520 kb on - strand, within MMJJ_RS03850 at 717.681 kb on + strand at 717.683 kb on + strand at 717.704 kb on + strand at 717.704 kb on + strand at 717.728 kb on - strand at 717.729 kb on + strand at 717.732 kb on + strand at 717.735 kb on - strand at 717.736 kb on + strand at 717.746 kb on - strand at 717.746 kb on - strand at 717.750 kb on - strand at 717.751 kb on + strand at 717.751 kb on + strand at 717.756 kb on - strand at 717.756 kb on - strand at 717.756 kb on - strand at 717.757 kb on + strand at 717.759 kb on + strand at 717.759 kb on + strand at 717.761 kb on - strand at 717.761 kb on - strand at 717.761 kb on - strand at 717.762 kb on + strand at 717.765 kb on - strand at 717.765 kb on - strand at 717.765 kb on - strand at 717.765 kb on - strand at 717.766 kb on + strand at 717.766 kb on + strand at 717.766 kb on + strand at 717.771 kb on - strand at 717.771 kb on - strand at 717.791 kb on - strand at 717.791 kb on - strand at 717.798 kb on + strand at 717.798 kb on + strand at 717.798 kb on + strand at 717.806 kb on + strand at 717.824 kb on + strand at 717.824 kb on + strand at 717.824 kb on + strand at 717.827 kb on - strand at 717.827 kb on - strand at 717.827 kb on - strand at 717.827 kb on - strand at 717.827 kb on - strand at 717.827 kb on - strand at 717.828 kb on + strand at 717.828 kb on + strand at 717.852 kb on + strand at 717.860 kb on + strand at 717.971 kb on - strand at 718.131 kb on - strand at 718.367 kb on - strand at 718.371 kb on - strand at 718.371 kb on - strand at 718.372 kb on + strand at 718.440 kb on + strand at 718.476 kb on - strand at 718.476 kb on - strand at 718.491 kb on - strand at 718.492 kb on + strand at 718.511 kb on - strand at 718.513 kb on - strand at 718.514 kb on + strand at 718.515 kb on - strand at 718.516 kb on + strand at 718.583 kb on - strand, within MMJJ_RS03860 at 718.653 kb on + strand, within MMJJ_RS03860 at 718.662 kb on + strand, within MMJJ_RS03860
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM D-glucosamine remove 716,712 + MMJJ_RS03845 0.78 -2.4 716,754 + +1.3 717,036 - -3.0 717,115 - -1.5 717,121 - +1.3 717,158 + -1.5 717,159 - -0.8 717,159 - -0.8 717,196 - -0.4 717,204 + +0.3 717,207 - -1.8 717,208 + -2.7 717,208 + -2.2 717,208 + -2.8 717,212 - -1.2 717,231 + -0.3 717,255 - -1.4 717,282 - -0.6 717,303 - MMJJ_RS03850 0.10 -0.2 717,304 + MMJJ_RS03850 0.10 +1.5 717,324 - MMJJ_RS03850 0.15 +1.0 717,324 - MMJJ_RS03850 0.15 -1.6 717,326 - MMJJ_RS03850 0.15 -3.4 717,340 - MMJJ_RS03850 0.19 -0.2 717,340 - MMJJ_RS03850 0.19 -0.5 717,341 + MMJJ_RS03850 0.19 -0.8 717,342 - MMJJ_RS03850 0.19 -0.0 717,392 - MMJJ_RS03850 0.31 -0.8 717,392 - MMJJ_RS03850 0.31 +1.2 717,392 - MMJJ_RS03850 0.31 -0.2 717,393 + MMJJ_RS03850 0.31 +0.5 717,393 + MMJJ_RS03850 0.31 -0.2 717,483 - MMJJ_RS03850 0.53 -0.2 717,520 - MMJJ_RS03850 0.62 -2.4 717,681 + +0.7 717,683 + -0.7 717,704 + -0.7 717,704 + -0.8 717,728 - -0.8 717,729 + +1.3 717,732 + +0.4 717,735 - +1.3 717,736 + +1.0 717,746 - -0.8 717,746 - +0.5 717,750 - -1.4 717,751 + -0.9 717,751 + +0.2 717,756 - -0.1 717,756 - -1.2 717,756 - +0.9 717,757 + -0.1 717,759 + -1.2 717,759 + -0.0 717,761 - -0.2 717,761 - +0.3 717,761 - -0.2 717,762 + -1.4 717,765 - -0.2 717,765 - +0.3 717,765 - +1.2 717,765 - +1.2 717,766 + +1.9 717,766 + -0.2 717,766 + -0.4 717,771 - -0.1 717,771 - +2.6 717,791 - -0.6 717,791 - -0.6 717,798 + +1.5 717,798 + -0.4 717,798 + -0.6 717,806 + +0.5 717,824 + -1.7 717,824 + -1.8 717,824 + -0.4 717,827 - -1.7 717,827 - -2.0 717,827 - -0.4 717,827 - +0.9 717,827 - -0.1 717,827 - +0.3 717,828 + +0.6 717,828 + -0.5 717,852 + -2.1 717,860 + +0.5 717,971 - +1.5 718,131 - -0.9 718,367 - +0.5 718,371 - +0.1 718,371 - -0.5 718,372 + +0.5 718,440 + +0.1 718,476 - -1.3 718,476 - -0.1 718,491 - +0.5 718,492 + -0.1 718,511 - -1.5 718,513 - +0.4 718,514 + -0.4 718,515 - +1.4 718,516 + -1.6 718,583 - MMJJ_RS03860 0.12 +0.4 718,653 + MMJJ_RS03860 0.16 -0.9 718,662 + MMJJ_RS03860 0.16 -0.6
Or see this region's nucleotide sequence