Experiment: H2 N-free with 10mM D-glucosamine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03455 and MMJJ_RS03460 are separated by 3 nucleotides MMJJ_RS03460 and MMJJ_RS03465 are separated by 120 nucleotides MMJJ_RS03465 and MMJJ_RS03470 are separated by 17 nucleotides
MMJJ_RS03455: MMJJ_RS03455 - DUF4868 domain-containing protein, at 647,698 to 648,720
_RS03455
MMJJ_RS03460: MMJJ_RS03460 - hypothetical protein, at 648,724 to 649,401
_RS03460
MMJJ_RS03465: MMJJ_RS03465 - restriction endonuclease, at 649,522 to 650,451
_RS03465
MMJJ_RS03470: MMJJ_RS03470 - N-6 DNA methylase, at 650,469 to 651,977
_RS03470
Position (kb) 649
650
651 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7 at 648.538 kb on - strand, within MMJJ_RS03455 at 648.596 kb on + strand, within MMJJ_RS03455 at 648.638 kb on + strand at 648.638 kb on + strand at 648.638 kb on + strand at 648.638 kb on + strand at 648.639 kb on - strand at 648.639 kb on - strand at 648.640 kb on + strand at 648.640 kb on + strand at 648.642 kb on - strand at 648.652 kb on + strand at 648.677 kb on - strand at 648.677 kb on - strand at 648.677 kb on - strand at 648.677 kb on - strand at 648.678 kb on + strand at 648.678 kb on + strand at 648.678 kb on + strand at 648.679 kb on - strand at 648.679 kb on - strand at 648.679 kb on - strand at 648.679 kb on - strand at 648.680 kb on + strand at 648.680 kb on + strand at 648.680 kb on + strand at 648.680 kb on + strand at 648.680 kb on + strand at 648.681 kb on - strand at 648.681 kb on - strand at 648.682 kb on + strand at 648.694 kb on - strand at 648.699 kb on - strand at 648.699 kb on - strand at 648.699 kb on - strand at 648.699 kb on - strand at 648.700 kb on + strand at 648.700 kb on + strand at 648.700 kb on + strand at 648.700 kb on + strand at 648.712 kb on - strand at 648.725 kb on - strand at 648.745 kb on + strand at 648.752 kb on - strand at 648.756 kb on - strand at 648.756 kb on - strand at 648.756 kb on - strand at 648.756 kb on - strand at 648.756 kb on - strand at 648.757 kb on + strand at 648.757 kb on + strand at 648.757 kb on + strand at 648.757 kb on + strand at 648.763 kb on - strand at 648.763 kb on - strand at 648.770 kb on + strand at 648.775 kb on + strand at 648.822 kb on + strand, within MMJJ_RS03460 at 648.843 kb on - strand, within MMJJ_RS03460 at 648.958 kb on - strand, within MMJJ_RS03460 at 648.963 kb on - strand, within MMJJ_RS03460 at 648.964 kb on + strand, within MMJJ_RS03460 at 648.976 kb on + strand, within MMJJ_RS03460 at 648.988 kb on + strand, within MMJJ_RS03460 at 649.017 kb on - strand, within MMJJ_RS03460 at 649.017 kb on - strand, within MMJJ_RS03460 at 649.017 kb on - strand, within MMJJ_RS03460 at 649.017 kb on - strand, within MMJJ_RS03460 at 649.017 kb on - strand, within MMJJ_RS03460 at 649.018 kb on + strand, within MMJJ_RS03460 at 649.018 kb on + strand, within MMJJ_RS03460 at 649.018 kb on + strand, within MMJJ_RS03460 at 649.019 kb on - strand, within MMJJ_RS03460 at 649.077 kb on + strand, within MMJJ_RS03460 at 649.093 kb on + strand, within MMJJ_RS03460 at 649.102 kb on + strand, within MMJJ_RS03460 at 649.134 kb on - strand, within MMJJ_RS03460 at 649.148 kb on + strand, within MMJJ_RS03460 at 649.155 kb on - strand, within MMJJ_RS03460 at 649.224 kb on - strand, within MMJJ_RS03460 at 649.232 kb on + strand, within MMJJ_RS03460 at 649.248 kb on - strand, within MMJJ_RS03460 at 649.248 kb on - strand, within MMJJ_RS03460 at 649.248 kb on - strand, within MMJJ_RS03460 at 649.249 kb on + strand, within MMJJ_RS03460 at 649.272 kb on + strand, within MMJJ_RS03460 at 649.295 kb on + strand, within MMJJ_RS03460 at 649.318 kb on - strand, within MMJJ_RS03460 at 649.331 kb on + strand, within MMJJ_RS03460 at 649.342 kb on - strand at 649.343 kb on + strand at 649.343 kb on + strand at 649.356 kb on - strand at 649.356 kb on - strand at 649.434 kb on + strand at 649.443 kb on - strand at 649.443 kb on - strand at 649.443 kb on - strand at 649.443 kb on - strand at 649.443 kb on - strand at 649.444 kb on + strand at 649.444 kb on + strand at 649.444 kb on + strand at 649.445 kb on - strand at 649.445 kb on - strand at 649.445 kb on - strand at 649.445 kb on - strand at 649.445 kb on - strand at 649.445 kb on - strand at 649.445 kb on - strand at 649.445 kb on - strand at 649.445 kb on - strand at 649.445 kb on - strand at 649.445 kb on - strand at 649.445 kb on - strand at 649.445 kb on - strand at 649.445 kb on - strand at 649.446 kb on + strand at 649.446 kb on + strand at 649.446 kb on + strand at 649.446 kb on + strand at 649.446 kb on + strand at 649.446 kb on + strand at 649.446 kb on + strand at 649.446 kb on + strand at 649.446 kb on + strand at 649.446 kb on + strand at 649.446 kb on + strand at 649.446 kb on + strand at 649.446 kb on + strand at 649.446 kb on + strand at 649.447 kb on - strand at 649.447 kb on - strand at 649.447 kb on - strand at 649.447 kb on - strand at 649.447 kb on - strand at 649.448 kb on + strand at 649.448 kb on + strand at 649.448 kb on + strand at 649.452 kb on - strand at 649.453 kb on + strand at 649.467 kb on + strand at 649.470 kb on + strand at 649.482 kb on + strand at 649.488 kb on + strand at 649.488 kb on + strand at 649.508 kb on + strand at 649.519 kb on + strand at 649.551 kb on - strand at 649.552 kb on + strand at 649.552 kb on + strand at 649.553 kb on - strand at 649.553 kb on - strand at 649.553 kb on - strand at 649.553 kb on - strand at 649.553 kb on - strand at 649.553 kb on - strand at 649.553 kb on - strand at 649.554 kb on + strand at 649.554 kb on + strand at 649.554 kb on + strand at 649.558 kb on - strand at 649.558 kb on - strand at 649.559 kb on + strand at 649.559 kb on + strand at 649.559 kb on + strand at 649.559 kb on + strand at 649.559 kb on + strand at 649.609 kb on + strand at 649.609 kb on + strand at 649.619 kb on - strand, within MMJJ_RS03465 at 649.620 kb on + strand, within MMJJ_RS03465 at 649.620 kb on + strand, within MMJJ_RS03465 at 649.632 kb on - strand, within MMJJ_RS03465 at 649.633 kb on + strand, within MMJJ_RS03465 at 649.633 kb on + strand, within MMJJ_RS03465 at 649.644 kb on - strand, within MMJJ_RS03465 at 649.658 kb on - strand, within MMJJ_RS03465 at 649.658 kb on - strand, within MMJJ_RS03465 at 649.659 kb on + strand, within MMJJ_RS03465 at 649.659 kb on + strand, within MMJJ_RS03465 at 649.659 kb on + strand, within MMJJ_RS03465 at 649.660 kb on - strand, within MMJJ_RS03465 at 649.660 kb on - strand, within MMJJ_RS03465 at 649.661 kb on + strand, within MMJJ_RS03465 at 649.661 kb on + strand, within MMJJ_RS03465 at 649.674 kb on - strand, within MMJJ_RS03465 at 649.674 kb on - strand, within MMJJ_RS03465 at 649.674 kb on - strand, within MMJJ_RS03465 at 649.674 kb on - strand, within MMJJ_RS03465 at 649.675 kb on + strand, within MMJJ_RS03465 at 649.675 kb on + strand, within MMJJ_RS03465 at 649.738 kb on + strand, within MMJJ_RS03465 at 649.751 kb on + strand, within MMJJ_RS03465 at 649.800 kb on - strand, within MMJJ_RS03465 at 649.809 kb on - strand, within MMJJ_RS03465 at 649.809 kb on - strand, within MMJJ_RS03465 at 649.852 kb on + strand, within MMJJ_RS03465 at 649.854 kb on + strand, within MMJJ_RS03465 at 649.883 kb on - strand, within MMJJ_RS03465 at 649.884 kb on + strand, within MMJJ_RS03465 at 649.911 kb on - strand, within MMJJ_RS03465 at 649.943 kb on + strand at 649.951 kb on + strand at 649.951 kb on + strand, within MMJJ_RS03465 at 649.992 kb on - strand, within MMJJ_RS03465 at 650.005 kb on + strand, within MMJJ_RS03465 at 650.049 kb on - strand, within MMJJ_RS03465 at 650.050 kb on + strand, within MMJJ_RS03465 at 650.097 kb on + strand, within MMJJ_RS03465 at 650.097 kb on + strand, within MMJJ_RS03465 at 650.146 kb on + strand, within MMJJ_RS03465 at 650.146 kb on + strand, within MMJJ_RS03465 at 650.215 kb on - strand, within MMJJ_RS03465 at 650.216 kb on + strand, within MMJJ_RS03465 at 650.216 kb on + strand, within MMJJ_RS03465 at 650.216 kb on + strand, within MMJJ_RS03465 at 650.216 kb on + strand, within MMJJ_RS03465 at 650.230 kb on - strand, within MMJJ_RS03465 at 650.231 kb on + strand, within MMJJ_RS03465 at 650.255 kb on - strand, within MMJJ_RS03465 at 650.256 kb on + strand, within MMJJ_RS03465 at 650.259 kb on - strand, within MMJJ_RS03465 at 650.259 kb on - strand, within MMJJ_RS03465 at 650.259 kb on - strand, within MMJJ_RS03465 at 650.259 kb on - strand, within MMJJ_RS03465 at 650.260 kb on + strand, within MMJJ_RS03465 at 650.260 kb on + strand, within MMJJ_RS03465 at 650.260 kb on + strand, within MMJJ_RS03465 at 650.260 kb on + strand, within MMJJ_RS03465 at 650.262 kb on + strand, within MMJJ_RS03465 at 650.268 kb on + strand, within MMJJ_RS03465 at 650.281 kb on + strand, within MMJJ_RS03465 at 650.308 kb on - strand, within MMJJ_RS03465 at 650.309 kb on + strand, within MMJJ_RS03465 at 650.370 kb on - strand at 650.370 kb on - strand at 650.383 kb on + strand at 650.424 kb on - strand at 650.425 kb on + strand at 650.428 kb on - strand at 650.475 kb on + strand at 650.478 kb on + strand at 650.478 kb on + strand at 650.478 kb on + strand at 650.478 kb on + strand at 650.483 kb on - strand at 650.484 kb on + strand at 650.484 kb on + strand at 650.487 kb on + strand at 650.657 kb on - strand, within MMJJ_RS03470 at 650.719 kb on - strand, within MMJJ_RS03470 at 651.147 kb on + strand, within MMJJ_RS03470 at 651.197 kb on - strand, within MMJJ_RS03470
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM D-glucosamine remove 648,538 - MMJJ_RS03455 0.82 -0.5 648,596 + MMJJ_RS03455 0.88 -0.4 648,638 + +0.6 648,638 + +1.0 648,638 + +0.5 648,638 + -0.3 648,639 - -0.5 648,639 - +0.5 648,640 + -0.8 648,640 + +0.1 648,642 - -0.8 648,652 + +0.1 648,677 - +0.3 648,677 - +1.0 648,677 - +0.6 648,677 - -0.1 648,678 + +2.5 648,678 + -0.4 648,678 + +0.2 648,679 - +0.2 648,679 - +0.3 648,679 - -1.5 648,679 - +0.1 648,680 + -0.8 648,680 + +2.7 648,680 + +0.5 648,680 + +0.4 648,680 + -1.4 648,681 - +0.9 648,681 - -0.9 648,682 + -1.9 648,694 - -0.4 648,699 - +0.5 648,699 - -1.1 648,699 - +0.1 648,699 - -0.4 648,700 + -0.3 648,700 + -0.6 648,700 + +0.2 648,700 + +0.1 648,712 - -1.5 648,725 - -0.4 648,745 + +1.1 648,752 - +0.3 648,756 - -0.7 648,756 - +1.1 648,756 - -0.5 648,756 - -0.9 648,756 - +0.3 648,757 + +0.8 648,757 + +0.1 648,757 + +1.2 648,757 + -0.4 648,763 - -1.0 648,763 - +1.9 648,770 + +0.8 648,775 + +2.4 648,822 + MMJJ_RS03460 0.14 -1.5 648,843 - MMJJ_RS03460 0.18 +0.7 648,958 - MMJJ_RS03460 0.35 +0.0 648,963 - MMJJ_RS03460 0.35 -1.0 648,964 + MMJJ_RS03460 0.35 +0.8 648,976 + MMJJ_RS03460 0.37 +0.2 648,988 + MMJJ_RS03460 0.39 +0.4 649,017 - MMJJ_RS03460 0.43 +1.0 649,017 - MMJJ_RS03460 0.43 +0.8 649,017 - MMJJ_RS03460 0.43 -0.5 649,017 - MMJJ_RS03460 0.43 +0.9 649,017 - MMJJ_RS03460 0.43 -1.0 649,018 + MMJJ_RS03460 0.43 +0.3 649,018 + MMJJ_RS03460 0.43 +0.0 649,018 + MMJJ_RS03460 0.43 +0.1 649,019 - MMJJ_RS03460 0.44 +0.7 649,077 + MMJJ_RS03460 0.52 +0.4 649,093 + MMJJ_RS03460 0.54 +1.4 649,102 + MMJJ_RS03460 0.56 +0.5 649,134 - MMJJ_RS03460 0.60 -1.6 649,148 + MMJJ_RS03460 0.63 +0.4 649,155 - MMJJ_RS03460 0.64 +0.4 649,224 - MMJJ_RS03460 0.74 +0.7 649,232 + MMJJ_RS03460 0.75 +0.3 649,248 - MMJJ_RS03460 0.77 +0.1 649,248 - MMJJ_RS03460 0.77 +1.0 649,248 - MMJJ_RS03460 0.77 +1.6 649,249 + MMJJ_RS03460 0.77 +0.8 649,272 + MMJJ_RS03460 0.81 -0.6 649,295 + MMJJ_RS03460 0.84 +0.1 649,318 - MMJJ_RS03460 0.88 -2.3 649,331 + MMJJ_RS03460 0.90 +0.2 649,342 - +0.8 649,343 + +1.6 649,343 + -0.3 649,356 - +0.0 649,356 - -0.2 649,434 + +1.0 649,443 - +1.5 649,443 - +2.6 649,443 - -2.7 649,443 - -1.1 649,443 - -1.5 649,444 + +1.3 649,444 + -0.2 649,444 + -0.9 649,445 - +0.8 649,445 - -0.5 649,445 - +0.3 649,445 - +3.5 649,445 - +0.8 649,445 - +1.7 649,445 - -1.6 649,445 - +0.6 649,445 - -1.0 649,445 - -0.9 649,445 - +0.3 649,445 - +0.8 649,445 - +0.1 649,445 - -0.8 649,446 + -0.8 649,446 + -0.6 649,446 + -0.2 649,446 + +0.7 649,446 + +0.5 649,446 + -1.1 649,446 + +0.2 649,446 + -1.1 649,446 + +2.1 649,446 + -1.4 649,446 + -0.9 649,446 + +0.6 649,446 + +0.5 649,446 + -0.5 649,447 - +0.5 649,447 - +0.4 649,447 - +0.4 649,447 - +0.0 649,447 - +0.0 649,448 + -0.5 649,448 + +1.6 649,448 + -0.0 649,452 - -0.1 649,453 + -0.4 649,467 + +0.8 649,470 + -0.2 649,482 + -0.5 649,488 + +0.4 649,488 + -1.1 649,508 + +0.6 649,519 + +0.2 649,551 - -0.4 649,552 + -2.4 649,552 + -1.8 649,553 - +0.2 649,553 - +1.2 649,553 - -0.3 649,553 - -0.3 649,553 - +0.5 649,553 - -0.6 649,553 - -1.6 649,554 + +1.0 649,554 + -0.1 649,554 + +2.3 649,558 - -0.5 649,558 - +3.1 649,559 + -0.1 649,559 + +1.0 649,559 + +0.2 649,559 + +0.9 649,559 + +0.1 649,609 + -1.0 649,609 + -0.7 649,619 - MMJJ_RS03465 0.10 -0.6 649,620 + MMJJ_RS03465 0.11 +0.4 649,620 + MMJJ_RS03465 0.11 +1.1 649,632 - MMJJ_RS03465 0.12 -0.8 649,633 + MMJJ_RS03465 0.12 +0.4 649,633 + MMJJ_RS03465 0.12 +1.0 649,644 - MMJJ_RS03465 0.13 +1.1 649,658 - MMJJ_RS03465 0.15 -0.2 649,658 - MMJJ_RS03465 0.15 +0.3 649,659 + MMJJ_RS03465 0.15 +0.4 649,659 + MMJJ_RS03465 0.15 +0.8 649,659 + MMJJ_RS03465 0.15 +0.4 649,660 - MMJJ_RS03465 0.15 +0.3 649,660 - MMJJ_RS03465 0.15 -0.6 649,661 + MMJJ_RS03465 0.15 -0.3 649,661 + MMJJ_RS03465 0.15 +0.4 649,674 - MMJJ_RS03465 0.16 -1.3 649,674 - MMJJ_RS03465 0.16 -0.0 649,674 - MMJJ_RS03465 0.16 +0.3 649,674 - MMJJ_RS03465 0.16 +1.6 649,675 + MMJJ_RS03465 0.16 -0.2 649,675 + MMJJ_RS03465 0.16 +0.2 649,738 + MMJJ_RS03465 0.23 +1.9 649,751 + MMJJ_RS03465 0.25 +0.0 649,800 - MMJJ_RS03465 0.30 +7.3 649,809 - MMJJ_RS03465 0.31 +0.1 649,809 - MMJJ_RS03465 0.31 +0.7 649,852 + MMJJ_RS03465 0.35 +1.1 649,854 + MMJJ_RS03465 0.36 -0.6 649,883 - MMJJ_RS03465 0.39 +1.0 649,884 + MMJJ_RS03465 0.39 -0.8 649,911 - MMJJ_RS03465 0.42 -0.3 649,943 + +0.4 649,951 + +3.0 649,951 + MMJJ_RS03465 0.46 -0.2 649,992 - MMJJ_RS03465 0.51 +1.2 650,005 + MMJJ_RS03465 0.52 +1.1 650,049 - MMJJ_RS03465 0.57 +1.6 650,050 + MMJJ_RS03465 0.57 +0.4 650,097 + MMJJ_RS03465 0.62 +0.6 650,097 + MMJJ_RS03465 0.62 -0.9 650,146 + MMJJ_RS03465 0.67 +0.4 650,146 + MMJJ_RS03465 0.67 +2.6 650,215 - MMJJ_RS03465 0.75 -0.8 650,216 + MMJJ_RS03465 0.75 +0.4 650,216 + MMJJ_RS03465 0.75 +0.2 650,216 + MMJJ_RS03465 0.75 -0.1 650,216 + MMJJ_RS03465 0.75 +0.0 650,230 - MMJJ_RS03465 0.76 -0.2 650,231 + MMJJ_RS03465 0.76 +0.4 650,255 - MMJJ_RS03465 0.79 +0.2 650,256 + MMJJ_RS03465 0.79 -0.4 650,259 - MMJJ_RS03465 0.79 +0.5 650,259 - MMJJ_RS03465 0.79 +0.8 650,259 - MMJJ_RS03465 0.79 +0.4 650,259 - MMJJ_RS03465 0.79 +0.1 650,260 + MMJJ_RS03465 0.79 -1.2 650,260 + MMJJ_RS03465 0.79 +0.2 650,260 + MMJJ_RS03465 0.79 +0.1 650,260 + MMJJ_RS03465 0.79 -0.3 650,262 + MMJJ_RS03465 0.80 -0.0 650,268 + MMJJ_RS03465 0.80 -0.2 650,281 + MMJJ_RS03465 0.82 -2.2 650,308 - MMJJ_RS03465 0.85 +0.8 650,309 + MMJJ_RS03465 0.85 +0.4 650,370 - +0.8 650,370 - +0.8 650,383 + +0.6 650,424 - -0.8 650,425 + +1.0 650,428 - -0.0 650,475 + +1.5 650,478 + -0.9 650,478 + -0.3 650,478 + -0.8 650,478 + -0.5 650,483 - +1.1 650,484 + -1.0 650,484 + -0.2 650,487 + -0.1 650,657 - MMJJ_RS03470 0.12 +1.0 650,719 - MMJJ_RS03470 0.17 +1.4 651,147 + MMJJ_RS03470 0.45 -0.6 651,197 - MMJJ_RS03470 0.48 -0.0
Or see this region's nucleotide sequence