Experiment: H2 N-free with 10mM D-glucosamine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01860 and MMJJ_RS01865 are separated by 147 nucleotides MMJJ_RS01865 and MMJJ_RS01870 are separated by 135 nucleotides
MMJJ_RS01860: MMJJ_RS01860 - tRNA guanosine(15) transglycosylase TgtA, at 356,203 to 358,152
_RS01860
MMJJ_RS01865: MMJJ_RS01865 - peptidyl-tRNA hydrolase Pth2, at 358,300 to 358,650
_RS01865
MMJJ_RS01870: MMJJ_RS01870 - methanogenesis marker 15 protein, at 358,786 to 360,021
_RS01870
Position (kb) 358
359 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6 at 357.418 kb on - strand, within MMJJ_RS01860 at 357.451 kb on - strand, within MMJJ_RS01860 at 357.568 kb on - strand, within MMJJ_RS01860 at 357.568 kb on - strand, within MMJJ_RS01860 at 357.585 kb on + strand, within MMJJ_RS01860 at 357.588 kb on - strand, within MMJJ_RS01860 at 357.609 kb on - strand, within MMJJ_RS01860 at 357.679 kb on + strand at 357.703 kb on + strand, within MMJJ_RS01860 at 357.723 kb on - strand, within MMJJ_RS01860 at 357.724 kb on + strand, within MMJJ_RS01860 at 357.736 kb on - strand, within MMJJ_RS01860 at 357.737 kb on + strand, within MMJJ_RS01860 at 357.737 kb on + strand, within MMJJ_RS01860 at 357.737 kb on + strand, within MMJJ_RS01860 at 357.783 kb on - strand, within MMJJ_RS01860 at 357.854 kb on + strand, within MMJJ_RS01860 at 357.854 kb on + strand, within MMJJ_RS01860 at 357.971 kb on - strand at 357.972 kb on + strand at 357.977 kb on + strand at 357.977 kb on + strand at 357.995 kb on + strand at 358.019 kb on + strand at 358.060 kb on - strand at 358.109 kb on + strand at 358.120 kb on + strand at 358.163 kb on - strand at 358.164 kb on + strand at 358.164 kb on + strand at 358.168 kb on - strand at 358.168 kb on - strand at 358.176 kb on + strand at 358.176 kb on + strand at 358.176 kb on + strand at 358.177 kb on - strand at 358.177 kb on - strand at 358.178 kb on + strand at 358.184 kb on - strand at 358.184 kb on - strand at 358.207 kb on - strand at 358.207 kb on - strand at 358.220 kb on - strand at 358.225 kb on - strand at 358.226 kb on + strand at 358.232 kb on - strand at 358.232 kb on - strand at 358.233 kb on + strand at 358.233 kb on + strand at 358.233 kb on + strand at 358.233 kb on + strand at 358.236 kb on - strand at 358.236 kb on - strand at 358.239 kb on - strand at 358.239 kb on - strand at 358.239 kb on - strand at 358.240 kb on + strand at 358.243 kb on - strand at 358.243 kb on - strand at 358.243 kb on - strand at 358.243 kb on - strand at 358.243 kb on - strand at 358.244 kb on + strand at 358.244 kb on + strand at 358.244 kb on + strand at 358.244 kb on + strand at 358.244 kb on + strand at 358.244 kb on + strand at 358.245 kb on - strand at 358.245 kb on - strand at 358.246 kb on + strand at 358.246 kb on + strand at 358.248 kb on - strand at 358.248 kb on - strand at 358.248 kb on - strand at 358.249 kb on + strand at 358.249 kb on + strand at 358.255 kb on - strand at 358.255 kb on - strand at 358.256 kb on + strand at 358.258 kb on + strand at 358.320 kb on - strand at 358.335 kb on + strand, within MMJJ_RS01865 at 358.335 kb on + strand, within MMJJ_RS01865 at 358.335 kb on + strand, within MMJJ_RS01865 at 358.410 kb on + strand, within MMJJ_RS01865 at 358.410 kb on + strand, within MMJJ_RS01865 at 358.470 kb on + strand, within MMJJ_RS01865 at 358.497 kb on + strand, within MMJJ_RS01865 at 358.535 kb on - strand, within MMJJ_RS01865 at 358.536 kb on + strand, within MMJJ_RS01865 at 358.648 kb on - strand at 358.649 kb on + strand at 358.656 kb on + strand at 358.672 kb on - strand at 358.690 kb on + strand at 358.690 kb on + strand at 358.692 kb on - strand at 358.692 kb on - strand at 358.692 kb on - strand at 358.693 kb on + strand at 358.694 kb on - strand at 358.694 kb on - strand at 358.694 kb on - strand at 358.695 kb on + strand at 358.699 kb on + strand at 358.716 kb on - strand at 358.722 kb on + strand at 358.722 kb on + strand at 358.883 kb on - strand at 359.061 kb on + strand at 359.082 kb on + strand, within MMJJ_RS01870 at 359.528 kb on - strand, within MMJJ_RS01870 at 359.528 kb on - strand, within MMJJ_RS01870
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM D-glucosamine remove 357,418 - MMJJ_RS01860 0.62 -0.8 357,451 - MMJJ_RS01860 0.64 -1.1 357,568 - MMJJ_RS01860 0.70 -2.6 357,568 - MMJJ_RS01860 0.70 +0.2 357,585 + MMJJ_RS01860 0.71 -1.3 357,588 - MMJJ_RS01860 0.71 -0.3 357,609 - MMJJ_RS01860 0.72 -0.5 357,679 + -0.0 357,703 + MMJJ_RS01860 0.77 -1.1 357,723 - MMJJ_RS01860 0.78 -1.6 357,724 + MMJJ_RS01860 0.78 -0.0 357,736 - MMJJ_RS01860 0.79 -1.7 357,737 + MMJJ_RS01860 0.79 -2.5 357,737 + MMJJ_RS01860 0.79 -2.5 357,737 + MMJJ_RS01860 0.79 -2.3 357,783 - MMJJ_RS01860 0.81 -2.5 357,854 + MMJJ_RS01860 0.85 -0.6 357,854 + MMJJ_RS01860 0.85 -2.4 357,971 - -1.7 357,972 + -0.3 357,977 + -0.8 357,977 + -2.8 357,995 + -1.8 358,019 + -0.8 358,060 - -0.3 358,109 + -3.0 358,120 + -2.7 358,163 - -0.7 358,164 + -0.5 358,164 + -0.3 358,168 - -1.0 358,168 - +1.0 358,176 + -0.9 358,176 + +1.2 358,176 + +0.7 358,177 - -2.6 358,177 - -1.6 358,178 + -0.7 358,184 - +0.1 358,184 - +0.5 358,207 - +0.7 358,207 - +6.1 358,220 - -0.8 358,225 - -1.9 358,226 + +0.7 358,232 - -0.9 358,232 - +0.2 358,233 + -1.4 358,233 + -0.0 358,233 + -0.5 358,233 + +0.5 358,236 - +0.8 358,236 - +0.4 358,239 - -0.6 358,239 - +0.8 358,239 - +1.0 358,240 + -0.7 358,243 - +1.0 358,243 - -0.9 358,243 - +0.3 358,243 - -2.0 358,243 - -0.3 358,244 + -0.9 358,244 + +0.8 358,244 + -0.3 358,244 + -1.0 358,244 + +0.2 358,244 + -1.9 358,245 - -1.1 358,245 - -1.3 358,246 + -2.2 358,246 + -1.8 358,248 - -0.9 358,248 - -1.6 358,248 - -2.2 358,249 + +1.0 358,249 + +1.0 358,255 - -1.6 358,255 - -2.2 358,256 + -0.8 358,258 + -1.3 358,320 - -4.6 358,335 + MMJJ_RS01865 0.10 +0.4 358,335 + MMJJ_RS01865 0.10 -1.6 358,335 + MMJJ_RS01865 0.10 -2.7 358,410 + MMJJ_RS01865 0.31 -0.0 358,410 + MMJJ_RS01865 0.31 -3.5 358,470 + MMJJ_RS01865 0.48 -2.5 358,497 + MMJJ_RS01865 0.56 -1.5 358,535 - MMJJ_RS01865 0.67 -1.7 358,536 + MMJJ_RS01865 0.67 -0.9 358,648 - -0.7 358,649 + +0.7 358,656 + -0.2 358,672 - +0.9 358,690 + +0.3 358,690 + -2.0 358,692 - +1.6 358,692 - -0.4 358,692 - -0.7 358,693 + +0.4 358,694 - +1.2 358,694 - -0.4 358,694 - -1.0 358,695 + -0.8 358,699 + -0.9 358,716 - -1.6 358,722 + -0.6 358,722 + -1.3 358,883 - -1.2 359,061 + +1.0 359,082 + MMJJ_RS01870 0.24 -1.5 359,528 - MMJJ_RS01870 0.60 -0.3 359,528 - MMJJ_RS01870 0.60 -1.2
Or see this region's nucleotide sequence