Experiment: H2 N-free with 10mM D-glucosamine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00675 and MMJJ_RS00680 are separated by 16 nucleotides MMJJ_RS00680 and MMJJ_RS00685 are separated by 22 nucleotides
MMJJ_RS00675: MMJJ_RS00675 - nitrogenase, at 131,123 to 132,499
_RS00675
MMJJ_RS00680: MMJJ_RS00680 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifE, at 132,516 to 133,958
_RS00680
MMJJ_RS00685: MMJJ_RS00685 - nitrogenase molybdenum-iron protein subunit beta, at 133,981 to 135,369
_RS00685
Position (kb) 132
133
134 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 131.565 kb on + strand, within MMJJ_RS00675 at 131.656 kb on - strand, within MMJJ_RS00675 at 131.656 kb on - strand, within MMJJ_RS00675 at 131.656 kb on - strand, within MMJJ_RS00675 at 131.660 kb on - strand, within MMJJ_RS00675 at 131.660 kb on - strand, within MMJJ_RS00675 at 131.672 kb on - strand, within MMJJ_RS00675 at 131.686 kb on - strand, within MMJJ_RS00675 at 131.732 kb on - strand, within MMJJ_RS00675 at 131.733 kb on + strand, within MMJJ_RS00675 at 131.738 kb on - strand, within MMJJ_RS00675 at 131.742 kb on - strand, within MMJJ_RS00675 at 131.743 kb on + strand, within MMJJ_RS00675 at 131.806 kb on - strand, within MMJJ_RS00675 at 131.912 kb on + strand, within MMJJ_RS00675 at 131.945 kb on - strand, within MMJJ_RS00675 at 131.945 kb on - strand, within MMJJ_RS00675 at 132.018 kb on + strand, within MMJJ_RS00675 at 132.068 kb on + strand, within MMJJ_RS00675 at 132.097 kb on - strand, within MMJJ_RS00675 at 132.097 kb on - strand, within MMJJ_RS00675 at 132.097 kb on - strand, within MMJJ_RS00675 at 132.098 kb on + strand, within MMJJ_RS00675 at 132.098 kb on + strand, within MMJJ_RS00675 at 132.098 kb on + strand, within MMJJ_RS00675 at 132.098 kb on + strand, within MMJJ_RS00675 at 132.098 kb on + strand, within MMJJ_RS00675 at 132.098 kb on + strand, within MMJJ_RS00675 at 132.098 kb on + strand, within MMJJ_RS00675 at 132.098 kb on + strand, within MMJJ_RS00675 at 132.103 kb on - strand, within MMJJ_RS00675 at 132.103 kb on - strand, within MMJJ_RS00675 at 132.203 kb on + strand, within MMJJ_RS00675 at 132.215 kb on + strand, within MMJJ_RS00675 at 132.215 kb on + strand, within MMJJ_RS00675 at 132.219 kb on + strand, within MMJJ_RS00675 at 132.224 kb on - strand, within MMJJ_RS00675 at 132.225 kb on + strand, within MMJJ_RS00675 at 132.227 kb on - strand, within MMJJ_RS00675 at 132.372 kb on - strand at 132.372 kb on - strand at 132.372 kb on - strand at 132.372 kb on - strand at 132.373 kb on + strand at 132.373 kb on + strand at 132.399 kb on + strand at 132.399 kb on + strand at 132.399 kb on + strand at 132.438 kb on + strand at 132.438 kb on + strand at 132.458 kb on - strand at 132.458 kb on - strand at 132.459 kb on + strand at 132.459 kb on + strand at 132.473 kb on + strand at 132.502 kb on - strand at 132.688 kb on + strand, within MMJJ_RS00680 at 132.700 kb on - strand, within MMJJ_RS00680 at 132.717 kb on - strand, within MMJJ_RS00680 at 132.718 kb on + strand, within MMJJ_RS00680 at 132.718 kb on + strand, within MMJJ_RS00680 at 132.751 kb on + strand, within MMJJ_RS00680 at 132.755 kb on - strand, within MMJJ_RS00680 at 132.755 kb on - strand, within MMJJ_RS00680 at 132.755 kb on - strand, within MMJJ_RS00680 at 132.756 kb on + strand, within MMJJ_RS00680 at 132.756 kb on + strand, within MMJJ_RS00680 at 132.776 kb on - strand, within MMJJ_RS00680 at 132.777 kb on + strand, within MMJJ_RS00680 at 132.887 kb on + strand, within MMJJ_RS00680 at 132.888 kb on - strand, within MMJJ_RS00680 at 132.889 kb on + strand, within MMJJ_RS00680 at 132.903 kb on + strand, within MMJJ_RS00680 at 132.941 kb on - strand, within MMJJ_RS00680 at 133.035 kb on + strand, within MMJJ_RS00680 at 133.104 kb on - strand, within MMJJ_RS00680 at 133.108 kb on - strand, within MMJJ_RS00680 at 133.109 kb on + strand, within MMJJ_RS00680 at 133.134 kb on - strand, within MMJJ_RS00680 at 133.134 kb on - strand, within MMJJ_RS00680 at 133.136 kb on - strand, within MMJJ_RS00680 at 133.136 kb on - strand, within MMJJ_RS00680 at 133.137 kb on + strand, within MMJJ_RS00680 at 133.141 kb on + strand, within MMJJ_RS00680 at 133.146 kb on + strand, within MMJJ_RS00680 at 133.146 kb on + strand, within MMJJ_RS00680 at 133.190 kb on - strand, within MMJJ_RS00680 at 133.203 kb on - strand, within MMJJ_RS00680 at 133.203 kb on - strand, within MMJJ_RS00680 at 133.227 kb on - strand, within MMJJ_RS00680 at 133.235 kb on - strand, within MMJJ_RS00680 at 133.275 kb on - strand, within MMJJ_RS00680 at 133.277 kb on - strand, within MMJJ_RS00680 at 133.277 kb on - strand, within MMJJ_RS00680 at 133.277 kb on - strand, within MMJJ_RS00680 at 133.284 kb on + strand, within MMJJ_RS00680 at 133.323 kb on - strand, within MMJJ_RS00680 at 133.323 kb on - strand, within MMJJ_RS00680 at 133.327 kb on - strand, within MMJJ_RS00680 at 133.374 kb on + strand, within MMJJ_RS00680 at 133.439 kb on - strand, within MMJJ_RS00680 at 133.440 kb on + strand, within MMJJ_RS00680 at 133.452 kb on + strand, within MMJJ_RS00680 at 133.452 kb on + strand, within MMJJ_RS00680 at 133.473 kb on - strand, within MMJJ_RS00680 at 133.473 kb on - strand, within MMJJ_RS00680 at 133.476 kb on - strand, within MMJJ_RS00680 at 133.497 kb on - strand, within MMJJ_RS00680 at 133.520 kb on - strand, within MMJJ_RS00680 at 133.521 kb on + strand, within MMJJ_RS00680 at 133.567 kb on + strand, within MMJJ_RS00680 at 133.617 kb on + strand, within MMJJ_RS00680 at 133.625 kb on - strand, within MMJJ_RS00680 at 133.669 kb on + strand, within MMJJ_RS00680 at 133.689 kb on - strand, within MMJJ_RS00680 at 133.689 kb on - strand, within MMJJ_RS00680 at 133.690 kb on + strand, within MMJJ_RS00680 at 133.733 kb on + strand, within MMJJ_RS00680 at 133.784 kb on - strand, within MMJJ_RS00680 at 133.784 kb on - strand, within MMJJ_RS00680 at 133.836 kb on - strand at 133.847 kb on - strand at 133.890 kb on - strand at 133.891 kb on + strand at 133.891 kb on + strand at 133.911 kb on + strand at 133.958 kb on - strand at 133.959 kb on + strand at 133.961 kb on - strand at 133.961 kb on - strand at 133.961 kb on - strand at 133.962 kb on + strand at 133.982 kb on - strand at 133.983 kb on + strand at 133.983 kb on + strand at 134.015 kb on - strand at 134.038 kb on + strand at 134.130 kb on + strand, within MMJJ_RS00685 at 134.218 kb on - strand, within MMJJ_RS00685 at 134.219 kb on + strand, within MMJJ_RS00685 at 134.219 kb on + strand, within MMJJ_RS00685 at 134.219 kb on + strand, within MMJJ_RS00685 at 134.228 kb on + strand, within MMJJ_RS00685 at 134.228 kb on + strand, within MMJJ_RS00685 at 134.319 kb on - strand, within MMJJ_RS00685 at 134.319 kb on - strand, within MMJJ_RS00685 at 134.319 kb on - strand, within MMJJ_RS00685 at 134.320 kb on + strand, within MMJJ_RS00685 at 134.323 kb on - strand, within MMJJ_RS00685 at 134.323 kb on - strand, within MMJJ_RS00685 at 134.324 kb on + strand, within MMJJ_RS00685 at 134.324 kb on + strand, within MMJJ_RS00685 at 134.324 kb on + strand, within MMJJ_RS00685 at 134.372 kb on + strand, within MMJJ_RS00685 at 134.378 kb on + strand, within MMJJ_RS00685 at 134.378 kb on + strand, within MMJJ_RS00685 at 134.393 kb on - strand, within MMJJ_RS00685 at 134.393 kb on - strand, within MMJJ_RS00685 at 134.417 kb on + strand, within MMJJ_RS00685 at 134.420 kb on - strand, within MMJJ_RS00685 at 134.420 kb on - strand, within MMJJ_RS00685 at 134.421 kb on + strand, within MMJJ_RS00685 at 134.421 kb on + strand, within MMJJ_RS00685 at 134.473 kb on - strand, within MMJJ_RS00685 at 134.474 kb on + strand, within MMJJ_RS00685 at 134.474 kb on + strand, within MMJJ_RS00685 at 134.533 kb on + strand, within MMJJ_RS00685 at 134.580 kb on + strand, within MMJJ_RS00685 at 134.615 kb on - strand, within MMJJ_RS00685 at 134.616 kb on + strand, within MMJJ_RS00685 at 134.619 kb on - strand, within MMJJ_RS00685 at 134.634 kb on - strand, within MMJJ_RS00685 at 134.634 kb on - strand, within MMJJ_RS00685 at 134.635 kb on + strand, within MMJJ_RS00685 at 134.704 kb on + strand, within MMJJ_RS00685 at 134.749 kb on + strand, within MMJJ_RS00685 at 134.754 kb on - strand, within MMJJ_RS00685 at 134.755 kb on + strand, within MMJJ_RS00685 at 134.755 kb on + strand, within MMJJ_RS00685 at 134.758 kb on - strand, within MMJJ_RS00685 at 134.759 kb on + strand, within MMJJ_RS00685 at 134.764 kb on + strand, within MMJJ_RS00685 at 134.790 kb on - strand, within MMJJ_RS00685 at 134.790 kb on - strand, within MMJJ_RS00685 at 134.791 kb on + strand, within MMJJ_RS00685 at 134.791 kb on + strand, within MMJJ_RS00685 at 134.791 kb on + strand, within MMJJ_RS00685 at 134.821 kb on - strand, within MMJJ_RS00685 at 134.839 kb on + strand, within MMJJ_RS00685 at 134.839 kb on + strand, within MMJJ_RS00685 at 134.843 kb on + strand, within MMJJ_RS00685 at 134.875 kb on - strand, within MMJJ_RS00685 at 134.880 kb on - strand, within MMJJ_RS00685 at 134.881 kb on + strand, within MMJJ_RS00685 at 134.884 kb on - strand, within MMJJ_RS00685 at 134.885 kb on + strand, within MMJJ_RS00685 at 134.896 kb on - strand, within MMJJ_RS00685 at 134.905 kb on + strand, within MMJJ_RS00685 at 134.909 kb on - strand, within MMJJ_RS00685 at 134.943 kb on - strand, within MMJJ_RS00685 at 134.944 kb on + strand, within MMJJ_RS00685 at 134.946 kb on - strand, within MMJJ_RS00685 at 134.947 kb on + strand, within MMJJ_RS00685
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM D-glucosamine remove 131,565 + MMJJ_RS00675 0.32 -3.7 131,656 - MMJJ_RS00675 0.39 -3.0 131,656 - MMJJ_RS00675 0.39 -3.3 131,656 - MMJJ_RS00675 0.39 -3.1 131,660 - MMJJ_RS00675 0.39 -3.1 131,660 - MMJJ_RS00675 0.39 -3.9 131,672 - MMJJ_RS00675 0.40 -3.7 131,686 - MMJJ_RS00675 0.41 -2.3 131,732 - MMJJ_RS00675 0.44 -1.4 131,733 + MMJJ_RS00675 0.44 -2.4 131,738 - MMJJ_RS00675 0.45 -0.8 131,742 - MMJJ_RS00675 0.45 -3.3 131,743 + MMJJ_RS00675 0.45 -2.6 131,806 - MMJJ_RS00675 0.50 -0.3 131,912 + MMJJ_RS00675 0.57 -2.3 131,945 - MMJJ_RS00675 0.60 -2.6 131,945 - MMJJ_RS00675 0.60 -3.2 132,018 + MMJJ_RS00675 0.65 -4.8 132,068 + MMJJ_RS00675 0.69 -3.5 132,097 - MMJJ_RS00675 0.71 -3.6 132,097 - MMJJ_RS00675 0.71 -2.1 132,097 - MMJJ_RS00675 0.71 -2.7 132,098 + MMJJ_RS00675 0.71 -2.4 132,098 + MMJJ_RS00675 0.71 -2.7 132,098 + MMJJ_RS00675 0.71 -2.5 132,098 + MMJJ_RS00675 0.71 -1.2 132,098 + MMJJ_RS00675 0.71 -2.5 132,098 + MMJJ_RS00675 0.71 -0.2 132,098 + MMJJ_RS00675 0.71 -2.5 132,098 + MMJJ_RS00675 0.71 -0.6 132,103 - MMJJ_RS00675 0.71 -0.7 132,103 - MMJJ_RS00675 0.71 -3.2 132,203 + MMJJ_RS00675 0.78 -2.0 132,215 + MMJJ_RS00675 0.79 -2.8 132,215 + MMJJ_RS00675 0.79 -1.7 132,219 + MMJJ_RS00675 0.80 -3.3 132,224 - MMJJ_RS00675 0.80 -3.2 132,225 + MMJJ_RS00675 0.80 -2.6 132,227 - MMJJ_RS00675 0.80 -1.2 132,372 - -2.2 132,372 - -2.8 132,372 - -2.1 132,372 - -1.0 132,373 + -2.8 132,373 + -3.9 132,399 + -2.5 132,399 + -2.6 132,399 + -1.0 132,438 + -1.9 132,438 + -1.7 132,458 - -1.2 132,458 - -2.0 132,459 + -3.1 132,459 + -3.6 132,473 + -1.6 132,502 - -2.7 132,688 + MMJJ_RS00680 0.12 -1.4 132,700 - MMJJ_RS00680 0.13 -3.4 132,717 - MMJJ_RS00680 0.14 -1.1 132,718 + MMJJ_RS00680 0.14 -1.0 132,718 + MMJJ_RS00680 0.14 -2.2 132,751 + MMJJ_RS00680 0.16 -2.2 132,755 - MMJJ_RS00680 0.17 -2.7 132,755 - MMJJ_RS00680 0.17 -2.8 132,755 - MMJJ_RS00680 0.17 -2.0 132,756 + MMJJ_RS00680 0.17 -2.4 132,756 + MMJJ_RS00680 0.17 -3.6 132,776 - MMJJ_RS00680 0.18 -3.7 132,777 + MMJJ_RS00680 0.18 -3.5 132,887 + MMJJ_RS00680 0.26 -0.6 132,888 - MMJJ_RS00680 0.26 -2.5 132,889 + MMJJ_RS00680 0.26 -1.9 132,903 + MMJJ_RS00680 0.27 -3.0 132,941 - MMJJ_RS00680 0.29 -2.3 133,035 + MMJJ_RS00680 0.36 -3.3 133,104 - MMJJ_RS00680 0.41 -1.0 133,108 - MMJJ_RS00680 0.41 -1.3 133,109 + MMJJ_RS00680 0.41 +0.5 133,134 - MMJJ_RS00680 0.43 -2.3 133,134 - MMJJ_RS00680 0.43 -2.6 133,136 - MMJJ_RS00680 0.43 -2.4 133,136 - MMJJ_RS00680 0.43 -1.8 133,137 + MMJJ_RS00680 0.43 -3.1 133,141 + MMJJ_RS00680 0.43 -2.6 133,146 + MMJJ_RS00680 0.44 -5.1 133,146 + MMJJ_RS00680 0.44 -1.3 133,190 - MMJJ_RS00680 0.47 -2.4 133,203 - MMJJ_RS00680 0.48 -2.0 133,203 - MMJJ_RS00680 0.48 -2.6 133,227 - MMJJ_RS00680 0.49 -3.7 133,235 - MMJJ_RS00680 0.50 -2.9 133,275 - MMJJ_RS00680 0.53 -4.5 133,277 - MMJJ_RS00680 0.53 -1.7 133,277 - MMJJ_RS00680 0.53 -3.4 133,277 - MMJJ_RS00680 0.53 -3.0 133,284 + MMJJ_RS00680 0.53 -2.3 133,323 - MMJJ_RS00680 0.56 -1.4 133,323 - MMJJ_RS00680 0.56 -4.3 133,327 - MMJJ_RS00680 0.56 -2.3 133,374 + MMJJ_RS00680 0.59 -3.7 133,439 - MMJJ_RS00680 0.64 -1.4 133,440 + MMJJ_RS00680 0.64 -1.5 133,452 + MMJJ_RS00680 0.65 -3.9 133,452 + MMJJ_RS00680 0.65 -2.4 133,473 - MMJJ_RS00680 0.66 -4.5 133,473 - MMJJ_RS00680 0.66 -1.6 133,476 - MMJJ_RS00680 0.67 -1.8 133,497 - MMJJ_RS00680 0.68 -1.4 133,520 - MMJJ_RS00680 0.70 -2.9 133,521 + MMJJ_RS00680 0.70 -0.2 133,567 + MMJJ_RS00680 0.73 -4.2 133,617 + MMJJ_RS00680 0.76 -2.4 133,625 - MMJJ_RS00680 0.77 -2.9 133,669 + MMJJ_RS00680 0.80 -3.1 133,689 - MMJJ_RS00680 0.81 -3.6 133,689 - MMJJ_RS00680 0.81 -2.0 133,690 + MMJJ_RS00680 0.81 +7.1 133,733 + MMJJ_RS00680 0.84 -1.9 133,784 - MMJJ_RS00680 0.88 -4.0 133,784 - MMJJ_RS00680 0.88 -1.2 133,836 - -2.1 133,847 - -2.5 133,890 - -2.8 133,891 + -2.4 133,891 + -3.4 133,911 + +0.0 133,958 - -1.2 133,959 + -2.3 133,961 - -3.8 133,961 - -1.2 133,961 - -3.9 133,962 + -3.5 133,982 - -0.3 133,983 + -1.9 133,983 + -3.1 134,015 - -2.4 134,038 + -3.7 134,130 + MMJJ_RS00685 0.11 -1.1 134,218 - MMJJ_RS00685 0.17 -1.6 134,219 + MMJJ_RS00685 0.17 -2.3 134,219 + MMJJ_RS00685 0.17 -2.2 134,219 + MMJJ_RS00685 0.17 -2.4 134,228 + MMJJ_RS00685 0.18 -4.5 134,228 + MMJJ_RS00685 0.18 -3.8 134,319 - MMJJ_RS00685 0.24 -1.6 134,319 - MMJJ_RS00685 0.24 -2.6 134,319 - MMJJ_RS00685 0.24 -1.7 134,320 + MMJJ_RS00685 0.24 -3.2 134,323 - MMJJ_RS00685 0.25 -1.0 134,323 - MMJJ_RS00685 0.25 -1.8 134,324 + MMJJ_RS00685 0.25 -2.3 134,324 + MMJJ_RS00685 0.25 -4.3 134,324 + MMJJ_RS00685 0.25 -1.5 134,372 + MMJJ_RS00685 0.28 -2.9 134,378 + MMJJ_RS00685 0.29 -1.2 134,378 + MMJJ_RS00685 0.29 -2.2 134,393 - MMJJ_RS00685 0.30 -4.2 134,393 - MMJJ_RS00685 0.30 -1.1 134,417 + MMJJ_RS00685 0.31 -1.9 134,420 - MMJJ_RS00685 0.32 -2.3 134,420 - MMJJ_RS00685 0.32 -2.3 134,421 + MMJJ_RS00685 0.32 -2.2 134,421 + MMJJ_RS00685 0.32 -2.7 134,473 - MMJJ_RS00685 0.35 -2.4 134,474 + MMJJ_RS00685 0.35 -3.2 134,474 + MMJJ_RS00685 0.35 -2.7 134,533 + MMJJ_RS00685 0.40 -1.3 134,580 + MMJJ_RS00685 0.43 -2.5 134,615 - MMJJ_RS00685 0.46 -2.5 134,616 + MMJJ_RS00685 0.46 -0.8 134,619 - MMJJ_RS00685 0.46 -1.0 134,634 - MMJJ_RS00685 0.47 -2.6 134,634 - MMJJ_RS00685 0.47 -2.5 134,635 + MMJJ_RS00685 0.47 -1.0 134,704 + MMJJ_RS00685 0.52 -2.4 134,749 + MMJJ_RS00685 0.55 -1.6 134,754 - MMJJ_RS00685 0.56 -3.2 134,755 + MMJJ_RS00685 0.56 -3.5 134,755 + MMJJ_RS00685 0.56 -4.1 134,758 - MMJJ_RS00685 0.56 +0.1 134,759 + MMJJ_RS00685 0.56 -2.4 134,764 + MMJJ_RS00685 0.56 -2.2 134,790 - MMJJ_RS00685 0.58 -3.2 134,790 - MMJJ_RS00685 0.58 -1.2 134,791 + MMJJ_RS00685 0.58 -3.0 134,791 + MMJJ_RS00685 0.58 -2.4 134,791 + MMJJ_RS00685 0.58 -2.8 134,821 - MMJJ_RS00685 0.60 -2.8 134,839 + MMJJ_RS00685 0.62 -3.3 134,839 + MMJJ_RS00685 0.62 -1.0 134,843 + MMJJ_RS00685 0.62 -2.0 134,875 - MMJJ_RS00685 0.64 -1.2 134,880 - MMJJ_RS00685 0.65 -3.2 134,881 + MMJJ_RS00685 0.65 +3.6 134,884 - MMJJ_RS00685 0.65 -2.0 134,885 + MMJJ_RS00685 0.65 -3.2 134,896 - MMJJ_RS00685 0.66 -3.4 134,905 + MMJJ_RS00685 0.67 -1.2 134,909 - MMJJ_RS00685 0.67 -3.3 134,943 - MMJJ_RS00685 0.69 -3.6 134,944 + MMJJ_RS00685 0.69 -3.4 134,946 - MMJJ_RS00685 0.69 -2.5 134,947 + MMJJ_RS00685 0.70 -1.8
Or see this region's nucleotide sequence